Modular multi-wavelength LED based light source for hyperspectral imaging

Dolenec, R.; Rogelj, Luka; Stergar, Jošt; Milanič, Matija

doi:10.1117/12.2527075

Cited by 8 publications

(9 citation statements)

References 2 publications

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“…A custom-developed hyperspectral imaging setup [ 19 , 20 , 21 , 22 ], able to operate in both transmission and reflectance geometries, was used to acquire spectral and sample thickness data. In the case of the 1-week experiment, samples were imaged on a black foamed PVC substrate (Forex, Airex AG, Sins, Switzerland) and illuminated from above, recording reflectance data.…”

Section: Methodsmentioning

confidence: 99%

Molecular and Cellular Markers in Chlorhexidine-Induced Peritoneal Fibrosis in Mice

et al. 2022

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Understanding the tissue changes and molecular mechanisms of preclinical models is essential for creating an optimal experimental design for credible translation into clinics. In our study, a chlorhexidine (CHX)-induced mouse model of peritoneal fibrosis was used to analyze histological and molecular/cellular alterations induced by 1 and 3 weeks of intraperitoneal CHX application. CHX treatment for 1 week already caused injury, degradation, and loss of mesothelial cells, resulting in local inflammation, with the most severe structural changes occurring in the peritoneum around the ventral parts of the abdominal wall. The local inflammatory response in the abdominal wall showed no prominent differences between 1 and 3 weeks. We observed an increase in polymorphonuclear cells in the blood but no evidence of systemic inflammation as measured by serum levels of serum amyloid A and interleukin-6. CHX-induced fibrosis in the abdominal wall was more pronounced after 3 weeks, but the gene expression of fibrotic markers did not change over time. Complement system molecules were strongly expressed in the abdominal wall of CHX-treated mice. To conclude, both histological and molecular changes were already present in week 1, allowing examination at the onset of fibrosis. This is crucial information for refining further experiments and limiting the amount of unnecessary animal suffering.

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Section: Methodsmentioning

confidence: 99%

Molecular and Cellular Markers in Chlorhexidine-Induced Peritoneal Fibrosis in Mice

et al. 2022

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“…More details about the illumination can be found in Ref. 15 . Two-wire grid polarizers (Bolder Vision Optik) were fixed in front of the objective and LED panels in the cross-polarized configuration to eliminate specular reflectance.…”

Section: Methodsmentioning

confidence: 99%

Effect of curvature correction on parameters extracted from hyperspectral images

et al. 2021

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. Significance: Hyperspectral imaging (HSI) has emerged as a promising optical technique. Besides optical properties of a sample, other sample physical properties also affect the recorded images. They are significantly affected by the sample curvature and sample surface to camera distance. A correction method to reduce the artifacts is necessary to reliably extract sample properties. Aim: Our aim is to correct hyperspectral images using the three-dimensional (3D) surface data and assess how the correction affects the extracted sample properties. Approach: We propose the combination of HSI and 3D profilometry to correct the images using the Lambert cosine law. The feasibility of the correction method is presented first on hemispherical tissue phantoms and next on human hands before, during, and after the vascular occlusion test (VOT). Results: Seven different phantoms with known optical properties were created and imaged with a hyperspectral system. The correction method worked up to 60 deg inclination angle, whereas for uncorrected images the maximum angles were 20 deg. Imaging hands before, during, and after VOT shows good agreement between the expected and extracted skin physiological parameters. Conclusions: The correction method was successfully applied on the images of tissue phantoms of known optical properties and geometry and VOT. The proposed method could be applied to any reflectance optical imaging technique and should be used whenever the sample parameters need to be extracted from a curved surface sample.

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“…Hyperspectral images were acquired using a custom built push-broom HSI working in a reflectance geometry with a broadband LED light source covering the spectral range from 400 nm − 1000 nm [12,13]. The light source was configured to illuminate the whole sample homogeneously from two sides while using a combined diffusor/polarizer (Bolder Vision Optik, Inc., USA) in front of the LEDs.…”

Section: Hyperspectral Imagingmentioning

confidence: 99%

“…The first step in the processing workflow was pre-processing, which prepared raw digital number data from the hyperspectral camera for further analysis. The data was corrected for the nonlinearity of the detector [13] and normalized to the reflectance values using white and dark references [12]. Since the white reference used for measurements was made from PTFE (Dastaflon d.o.o., Slovenia), a correction of the reference measured using a Spectralon(R) spectroscopic standard (Labsphere Inc., USA) was also performed.…”

Section: Data Processingmentioning

confidence: 99%

Hyperspectral evaluation of peritoneal fibrosis in mouse models

Stergar¹,

Dolenec²,

Kojc³

et al. 2020

Biomed. Opt. Express

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Analysis of morphological changes of the peritoneal membrane is an essential part of animal studies when investigating molecular mechanisms involved in the development of peritoneal fibrosis or testing the effects of potential therapeutic agents. Current methods, such as histology and immunohistochemistry, require time consuming sample processing and analysis and result in limited spatial information. In this paper we present a new method to evaluate structural and chemical changes in an animal model of peritoneal fibrosis that is based on hyperspectral imaging and a model of light transport. The method is able to distinguish between healthy and diseased subjects based on morphological as well as physiological parameters such as blood and scattering parameters. Furthermore, it enables evaluation of changes, such as degree of inflammation and fibrosis, that are closely related to histological findings.

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Modular multi-wavelength LED based light source for hyperspectral imaging

Cited by 8 publications

References 2 publications

Molecular and Cellular Markers in Chlorhexidine-Induced Peritoneal Fibrosis in Mice

Molecular and Cellular Markers in Chlorhexidine-Induced Peritoneal Fibrosis in Mice

Effect of curvature correction on parameters extracted from hyperspectral images

Hyperspectral evaluation of peritoneal fibrosis in mouse models

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