Modular tissue engineering for the vascularization of subcutaneously transplanted pancreatic islets

Vlahos, Alexander E.; Cober, Nicholas D; Sefton, Michael V.

doi:10.1073/pnas.1619216114

Cited by 108 publications

(94 citation statements)

References 54 publications

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“…On day 14 following cell transplantation, the engrafted Matrigel was retrieved and processed for histological analyses. To determine whether the graft was perfused with functional vessels, Griffonia simplicifolia lectin, which is a protein that can be used to label vasculature by binding to carbohydrate components of the ECs, was injected into the tail vein 5 min before the animal was sacrificed 10…”

mentioning

confidence: 99%

Enhancement of Subcutaneously Transplanted β Cell Survival Using 3D Stem Cell Spheroids with Proangiogenic and Prosurvival Potential

Juang

Lin

et al. 2020

Adv. Biosys.

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Islet transplantation has been demonstrated to be a promising therapy for type 1 diabetes mellitus. Although it is a minimally invasive operating procedure and provides easy access for graft monitoring, subcutaneous transplantation of the islet only has limited therapeutic outcomes, owing to the poor capacity of skin tissue to foster revascularization in a short period. Herein, 3D cell spheroids of clinically accessible umbilical cord blood mesenchymal stem cells and human umbilical vein endothelial cells are formed and employed for codelivery with β cells subcutaneously. The 3D stem cell spheroids, which can secrete multiple proangiogenic and prosurvival growth factors, induce robust angiogenesis and prevent β cell graft death, as indicated by the results of in vivo bioluminescent tracking and histological analysis. These experimental data highlight the efficacy of the 3D stem cell spheroids that are fabricated using translationally applicable cell types in promoting the survival and function of subcutaneously transplanted β cells.

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mentioning

confidence: 99%

Enhancement of Subcutaneously Transplanted β Cell Survival Using 3D Stem Cell Spheroids with Proangiogenic and Prosurvival Potential

Juang

Lin

et al. 2020

Adv. Biosys.

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“…This model seems to result in EC retention and maintains the glucose stimulated Ca 2+ emissions . Another model involves harvesting islets and combining them with HUVECs, adipose‐derived MSCs, and collagen . The mixture is then cast and cultured for 3 days and then transplanted into diabetic mice.…”

Section: In Vitro Models Of Vascularized Organsmentioning

confidence: 99%

“…84 Another model involves harvesting islets and combining them with HUVECs, adipose-derived MSCs, and collagen. 85 The mixture is then cast and cultured for 3 days and then transplanted into diabetic mice. The mice who received the transplants were able to return to normoglycemia.…”

Section: Other Organsmentioning

confidence: 99%

Engineering tissue‐specific blood vessels

Herron

Hansen

Abaci

2019

Bioengineering & Transla Med

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Vascular diversity among organs has recently become widely recognized. Several studies using mouse and human fetal tissues revealed distinct characteristics of organ‐specific vasculature in molecular and functional levels. Thorough understanding of vascular heterogeneities in human adult tissues is significant for developing novel strategies for targeted drug delivery and tissue regeneration. Recent advancements in microfabrication techniques, biomaterials, and differentiation protocols allowed for incorporation of microvasculature into engineered organs. Such vascularized organ models represent physiologically relevant platforms that may offer innovative tools for dissecting the effects of the organ microenvironment on vascular development and expand our present knowledge on organ‐specific human vasculature. In this article, we provide an overview of the current structural and molecular evidence on microvascular diversity, bioengineering methods used to recapitulate the microenvironmental cues, and recent vascularized three‐dimensional organ models from the perspective of tissue‐specific vasculature.

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“…Some strategies have been used to overcome these limitations, including encapsulation, scaffolds, accessory cells, and/or trophic factors such as fibroblast growth factor (aFGF and bFGF) or vascular endothelial growth factor (VEGF) plus hepatocyte growth factor (HGF; Golocheikine et al, 2010;Kawakami et al, 2000;Kawakami et al, 2001;Sakata et al, 2014;Smink et al, 2017). In the last year, a few studies of subcutaneous islet transplantation have been published, highlighting the great interest of the scientific community in using this anatomical site (Bertuzzi & De Carlis, 2018;Farina et al, 2017;Hsu, Fu, & Wang, 2017;Komatsu et al, 2017;Pathak et al, 2017;Pepper et al, 2017;Perez-Basterrechea et al, 2017;Uematsu et al, 2018;Vlahos, Cober, & Sefton, 2017). However, there have been few attempts to apply this approach in humans.…”

Section: (D)mentioning

confidence: 99%

Tissue‐engineering approaches in pancreatic islet transplantation

Pérez-Basterrechea

Esteban

Vega

et al. 2018

Biotech & Bioengineering

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Pancreatic islet transplantation is a promising alternative to whole-pancreas transplantation as a treatment of type 1 diabetes mellitus. This technique has been extensively developed during the past few years, with the main purpose of minimizing the complications arising from the standard protocols used in organ transplantation. By using a variety of strategies used in tissue engineering and regenerative medicine, pancreatic islets have been successfully introduced in host patients with different outcomes in terms of islet survival and functionality, as well as the desired normoglycemic control. Here, we describe and discuss those strategies to transplant islets together with different scaffolds, in combination with various cell types and diffusible factors, and always with the aim of reducing host immune response and achieving islet survival, regardless of the site of transplantation.

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Modular tissue engineering for the vascularization of subcutaneously transplanted pancreatic islets

Cited by 108 publications

References 54 publications

Enhancement of Subcutaneously Transplanted β Cell Survival Using 3D Stem Cell Spheroids with Proangiogenic and Prosurvival Potential

Enhancement of Subcutaneously Transplanted β Cell Survival Using 3D Stem Cell Spheroids with Proangiogenic and Prosurvival Potential

Engineering tissue‐specific blood vessels

Tissue‐engineering approaches in pancreatic islet transplantation

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