Modulating cell adhesion to polybutylene succinate biotextile constructs for tissue engineering applications

Abstract

Textile-based technologies are powerful routes for the production of three-dimensional porous architectures for tissue engineering applications because of their feasibility and possibility for scaling-up. Herein, the use of knitting technology to produce polybutylene succinate fibre-based porous architectures is described. Furthermore, different treatments have been applied to functionalize the surface of the scaffolds developed: sodium hydroxide etching, ultraviolet radiation exposure in an ozone atmosphere and grafting (acrylic acid, vinyl phosphonic acid and vinyl sulphonic acid) after oxygen plasma activation as a way to tailor cell adhesion. A possible effect of the applied treatments on the bulk properties of the textile scaffolds has been considered and thus tensile tests in dry and hydrated states were also carried out. The microscopy results indicated that the surface morphology and roughness were affected by the applied treatments. The X-ray photoelectron spectroscopy and contact angle measurements showed the incorporation of oxygen-containing groups and higher surface free energy as result of the surface treatments applied. The DNA quantification and scanning electron microscopy analysis revealed that these modifications enhanced cell adhesion and altered cell morphology. Generally, sodium hydroxide treatment altered most significantly the surface properties, which in turn resulted in a high number of cells adherent to these surfaces. Based on the results obtained, the proposed surface treatments are appropriate to modify polybutylene succinate knitting scaffolds, influencing cell adhesion and its potential for use in tissue engineering applications. Copyright © 2016 John Wiley & Sons, Ltd.

Comparison between calcium carbonate and β‐tricalcium phosphate as additives of 3D printed scaffolds with polylactic acid matrix

“…However, the adhesive properties of cells after 7 days of culture appeared to be unaffected by the presence of the additives in the PLA‐based scaffolds (Figure ). A better understanding of this effect could be observed at early culture periods, since in the first 24 hr the surface properties dictate the first cell‐material interactions (Ribeiro et al, ). In this way, the main differences in relation to cell behaviour were observed in terms of metabolic activity, showing the PLA:CaCO 3 :β‐TCP 95:2.5:2.5 group of scaffolds a significantly higher metabolic activity of SaOS‐2 cells after 7 days of culture, as compared with the remaining PLA‐based constructs.…”

Knitted 3D Scaffolds of Polybutylene Succinate Support Human Mesenchymal Stem Cell Growth and Osteogenesis

“…From the beginning of the 1990s, PBS has been commercially produced for biodegradable package material [ 4 ], but during recent years, a question has been raised about its suitability for biomedical applications as well. Indeed, with respect to cell attachment, viability, and proliferation, pure PBS as well as PBS-chitosan and PBS- β -tricalcium phosphate composites have shown promising results with mouse and rat fibroblastic and osteoblastic cells as well as with human mesenchymal stem cells (hMSCs) [ 5 – 10 ]. However, only few reports have evaluated the cellular response of osteogenic differentiation on PBS-containing materials.…”

“…When considering the 3D architecture of the TE scaffolds, textile-based manufacturing strategies produce inherently porous and interconnected structures with high reproducibility and the possibility to easily scale up the production [ 10 ]. However, despite these advantages, textile technology is still a relatively new approach in the field of TE and it offers plenty of unexplored possibilities.…”

Silk-based anisotropical 3D biotextiles for bone regeneration

“…For example, Kellomäki et al, [35] developed composite plates of weft-knitted PLA meshes attached on the surface of PLA films, showing that the composite structures were able to guide new bone formation and the mechanical properties of the composites were dependent on the mesh component. In previous studies by our group [14,15,36], standard weft-knitted SF and polybutylene succinate (PBS) scaffolds were proposed as a platform for the functional engineering of bone, showing that the knitting textile substrates exhibit better extensibility or compliance as compared to other textile structures, including the 3D woven structures previously proposed by Moutos et al [9] as a possible strategy for cartilage TE applications. This technology has been applied for TE strategies that seek to restore the biomechanical functions of damaged musculoskeletal tissues [9,37,38], particularly difficult to mimic in terms of biomechanical properties, as they are continualy subjected to complex loading patterns that require tissue arquitectures with preferably aligned fibre structures, as those present on knitting structures.…”