Modulating the production of xylanase by Bacillus pumilus BS131 through optimization using waste fiber sludge

Kalim, Bushra; Ali, Nazish Mazhar; Iqbal, Asia; Zahid, Muhammad; Rehman, Saifur; Bashir, Nadia; Ali, R.

doi:10.1590/1519-6984.243874

Cited by 7 publications

(3 citation statements)

References 21 publications

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“…For both xylanase and cellulase production, the substrate concentration was also an important factor. The strain of Bacillus pumilus BS131 in the previous study produced the highest amount of xylanase (6.73±0.98 IU/ml) at 4% substrate concentration from wheat barn [27]. Arabinoxylan is the most abundant hemicellulose component, accounting for 10.9 -26.0 % of total wheat bran based on its dry matter [28].…”

Section: Main Factors Contribution and Their Interactionsmentioning

confidence: 88%

Factorial Analysis of Xylanase and Cellulase Production from Pineapple Peel Waste

Sivanesan

Arshad

Shariffuddin

et al. 2022

MSF

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Pineapple wastes (skin, core and crown) are mainly composed of carbohydrates (cellulose, hemicellulose, and lignin). Non-starch polysaccharides (NSP) in feed are indigestible by the endogenous enzymes in poultry. Thus, exogenous enzymes (xylanase and cellulase) are required to overcome this problem. Due to high fiber content, pineapple wastes are unsuitable for animal feed. However, the fermented waste juice could be used to produce enzymes. The objective of this study is to produce xylanase and cellulase from the fermentation of pineapple waste using Lactobacillus casei (L. casei) bacteria inoculated from probiotic drink. The fermentation was performed using different screening parameters (incubation time, temperature, pH value and substrate concentration) according to Two-Level Full Factorial Design (FFD) by Design Expert. From this study, the incubation temperature and substrate concentration had the highest influence on the xylanase activity (39.82 U/mL) while the fermentation time and substrate concentration mostly affected the cellulase activity (8.05 U/mL). Meanwhile, the pH had the least influence on both enzyme activities. The pineapple waste at its best fermentation parameters not only offers an economical way of high enzyme production but also alleviates the agricultural waste disposal issue. Further optimization of the pineapple waste fermentation parameters is required though to maximize enzyme production.

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Section: Main Factors Contribution and Their Interactionsmentioning

confidence: 88%

Factorial Analysis of Xylanase and Cellulase Production from Pineapple Peel Waste

Sivanesan

Arshad

Shariffuddin

et al. 2022

MSF

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show abstract

“…Bacillus strains are important industrial enzyme producers 16 , and many commercial enzymes including proteases, amylases, xylanases and lipases have been characterized and purified from different Bacillus species [17][18][19][20] . To explore the post-transcriptional regulation in Bacillus strains, we previously identified a set of sRNAs in Bacillus altitudinis SCU11, a productive strain of extracellular alkaline protease, through an RNA-seq-based approach and subsequent validation by northern blot 21 .…”

Section: Introductionmentioning

confidence: 99%

A linear and circular dual-conformation noncoding RNA involves in oxidative stress tolerance of Bacillus altitudinis

et al. 2023

Preprint

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Functional circular RNAs have been extensively studied in eukaryotes and progressively discovered in archaea, but not been identified in bacteria except some byproducts of self-spliced introns, devoid of biological function. In this study, using an experimental and computational pipeline, we confirm that a regulatory noncoding RNA DucS exists in both linear and circular conformations in Bacillus altitudinis. The 3’ end sequences of linear RNA are critical to RNA circularization. DucS promotes the tolerance of host to H2O2 stress through its linear forms, partly due to upregulating the translation of a stress-responsive gene htrA, whereas its circular forms can promote the degradation of linear cognates. Through re-evaluating published RNA-seq data from 30 bacteria and experimental identification, we demonstrate that circular RNAs also exist in other bacteria, occurred unexpectedly in some well-known noncoding RNAs. Our results reveal firstly a functional circular RNA in bacteria, highlighting the universal existence of functional circular RNAs in all three domains of life.

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“…Bacillus strains are important industrial enzyme producers 16 , and many commercial enzymes, including proteases, amylases, xylanases, and lipases, have been characterized and purified from different Bacillus species 17 – 20 . To explore posttranscriptional regulation in Bacillus strains, we previously identified a set of sRNAs in Bacillus altitudinis SCU11 (initially classified as Bacillus pumilus 21 ), a productive strain of extracellular alkaline protease, through an RNA-seq-based approach and subsequent validation by northern blotting 22 .…”

Section: Introductionmentioning

confidence: 99%

A linear and circular dual-conformation noncoding RNA involved in oxidative stress tolerance in Bacillus altitudinis

He,

Xu,

et al. 2023

Nat Commun

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Circular RNAs have been extensively studied in eukaryotes, but their presence and/or biological functionality in bacteria are unclear. Here, we show that a regulatory noncoding RNA (DucS) exists in both linear and circular conformation in Bacillus altitudinis. The linear forms promote B. altitudinis tolerance to H2O2 stress, partly through increased translation of a stress-responsive gene, htrA. The 3′ end sequences of the linear forms are crucial for RNA circularization, and formation of circular forms can decrease the levels of the regulatory linear cognates. Bioinformatic analysis of available RNA-seq datasets from 30 bacterial species revealed multiple circular RNA candidates, distinct from DucS, for all the examined species. Experiments testing for the presence of selected circular RNA candidates in four species successfully validated 7 out of 9 candidates from B. altitudinis and 4 out of 5 candidates from Bacillus paralicheniformis; However, none of the candidates tested for Bacillus subtilis and Escherichia coli were detected. Our work identifies a dual-conformation regulatory RNA in B. altitutidinis, and indicates that circular RNAs exist in diverse bacteria. However, circularization of specific RNAs does not seem to be conserved across species, and the circularization mechanisms and biological functionality of the circular forms remain unclear.

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Modulating the production of xylanase by Bacillus pumilus BS131 through optimization using waste fiber sludge

Cited by 7 publications

References 21 publications

Factorial Analysis of Xylanase and Cellulase Production from Pineapple Peel Waste

Factorial Analysis of Xylanase and Cellulase Production from Pineapple Peel Waste

A linear and circular dual-conformation noncoding RNA involves in oxidative stress tolerance of Bacillus altitudinis

A linear and circular dual-conformation noncoding RNA involved in oxidative stress tolerance in Bacillus altitudinis

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