Modulation of ACD6 dependent hyperimmunity by natural alleles of an Arabidopsis thaliana NLR resistance gene

Zhu, Weiren; Zaidem, Maricris; Weyer, Anna-Lena Van de; Gutaker, Rafał M.; Chae, Eunyoung; Kim, Sang‐Tae; Bemm, Felix; Li, Lei; Todesco, Marco; Schwab, Rebecca; Unger, Frederik; Beha, Marcel Janis; Demar, Monika; Weigel, Detlef

doi:10.1371/journal.pgen.1007628

Cited by 31 publications

(14 citation statements)

References 97 publications

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“…natural variation in the immune gene ACCELERATED CELL DEATH 6 (ACD6) is caused by a rare allele causing an extreme lesion phenotype. It is not yet clear what selective pressures influence ACD6 genetic variation (Todesco et al, 2010;Zhu et al, 2018). The contrast where Aliphatic GSL loci have high levels of allelic heterogeneity for independent and recurrent LOF and GOF events while other resistance genes have more stable biallelic variation suggests that there are different selective regimes influencing these loci.…”

Section: Discussionmentioning

confidence: 99%

Genetic variation, environment and demography intersect to shape Arabidopsis defense metabolite variation across Europe

Katz

Bagaza

Holden

et al. 2020

Preprint

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Plants face a variety of challenges within their ever-changing environment. Diverse metabolites are central to the plants ability to overcome these challenges. Understanding the environmental and genetic factors influencing the variation in specialized metabolites is the key to understand how plants survive and develop under changing environments. Here we measure the variation in specialized metabolites across a population of 797 natural Arabidopsis thaliana accessions. We show a combination of geography, environmental parameters, demography, and different genetic processes that creates a specific pattern in their accumulation and distribution. By identifying and tracking causal polymorphisms at multiple loci controlling metabolites variation we show that each locus displays extensive allelic heterogeneity with signatures of both parallel and convergent evolutionary processes. These loci combine epistatically and show differing relationships to environmental parameters leading to different distributions. This provides a detailed perspective about the complexity of the forces and mechanisms that shape the accumulation and distribution of a family of specialized metabolites critical for plant fitness.

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Section: Discussionmentioning

confidence: 99%

Genetic variation, environment and demography intersect to shape Arabidopsis defense metabolite variation across Europe

Katz

Bagaza

Holden

et al. 2020

Preprint

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“…Allelic interactions at the ACD6 (ACCELERATED CELL DEATH 6) locus in A. thaliana lead to hybrid necrosis and the enhanced expression of defense genes (Świadek et al 2017). In fact, ACD6 acts as a quantitative resistance gene that balances growth and pathogen resistance in natural populations of A. thaliana, and it has been shown that deleterious autoimmune ACD6 alleles are modulated by natural variants of SNC1 (Zhu et al 2018).…”

Section: Hr Cell Death As a Consequence Of Autoimmunitymentioning

confidence: 99%

Cell Death in Plant Immunity

Pitsili

Phukan

Coll

2019

Cold Spring Harb Perspect Biol

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Pathogen recognition by the plant immune system leads to defense responses that are often accompanied by a form of regulated cell death known as the hypersensitive response (HR). HR shares some features with regulated necrosis observed in animals. Genetically, HR can be uncoupled from local defense responses at the site of infection and its role in immunity may be to activate systemic responses in distal parts of the organism. Recent advances in the field reveal conserved cell death-specific signaling modules that are assembled by immune receptors in response to pathogen-derived effectors. The structural elucidation of the plant resistosome-an inflammasome-like structure that may attach to the plasma membrane on activation-opens the possibility that HR cell death is mediated by the formation of pores at the plasma membrane. Necrotrophic pathogens that feed on dead tissue have evolved strategies to trigger the HR cell death pathway as a survival strategy. Ectopic activation of immunomodulators during autoimmune reactions can also promote HR cell death. In this perspective, we discuss the role and regulation of HR in these different contexts.

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“…Epistatic interactions have been well-documented as underlying autoimmune responses and pathogen resistance in Arabidopsis (Bomblies et al, 2007;Alcázar et al, 2009;Chae et al, 2014;Zhu et al, 2018). Genetic analyses in these studies have implicated certain gene clusters or individual loci as important, but the biochemical or physical mechanism underlying the resultant phenotypes (e.g.…”

Section: Potential Epistatic Selection Of Gi CVI and Ztl Cvimentioning

confidence: 99%

Compensatory Mutations in GI and ZTL May Modulate Temperature Compensation in the Circadian Clock

et al. 2019

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Circadian systems share the three properties of entrainment, free-running period, and temperature compensation (TC). TC ensures nearly the same period over a broad range of physiologically relevant temperatures; however, the mechanisms behind TC remain poorly understood. Here, we identify single point mutations in two key elements of the Arabidopsis circadian clock, GIGANTEA (GI) and ZEITLUPE (ZTL), which likely act as compensatory substitutions to establish a remarkably constant free-running period over a wide range of temperatures. Using near-isogenic lines generated from the introgression of the Cape Verde Islands (Cvi) alleles of GI and ZTL into the Landsberg erecta (Ler) background, we show how longer periods in the Cvi background at higher temperatures correlate with a difference in strength of the GI/ZTL interaction. Pairwise interaction testing of all GI/ZTL allelic combinations shows similar affinities for isogenic alleles at 22°C, but very poor interaction between GI (Cvi) and ZTL (Cvi) at higher temperature. In vivo, this would result in lower ZTL levels at high temperatures leading to longer periods in the Cvi background. Mismatched allelic combinations result in extremely strong or weak GI/ZTL interactions, indicating how the corresponding natural variants likely became fixed through epistatic selection. Additionally, molecular characterization of GI (Cvi) reveals a novel functional motif that can modulate the GI/ZTL interaction as well as nucleocytoplasmic partitioning. Taken together, these results identify a plausible temperature-dependent molecular mechanism, which contributes to the robustness of TC through natural variation in GI and ZTL alleles found on the Cape Verde Islands.

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Modulation of ACD6 dependent hyperimmunity by natural alleles of an Arabidopsis thaliana NLR resistance gene

Cited by 31 publications

References 97 publications

Genetic variation, environment and demography intersect to shape Arabidopsis defense metabolite variation across Europe

Genetic variation, environment and demography intersect to shape Arabidopsis defense metabolite variation across Europe

Cell Death in Plant Immunity

Compensatory Mutations in GI and ZTL May Modulate Temperature Compensation in the Circadian Clock

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