Modulation of acetylcholinesterase activity using molecularly imprinted polymer nanoparticles

Abstract

Modulation of enzyme activity allows for control over many biological pathways and while strategies for the pharmaceutical design of inhibitors are well established; methods for promoting activation, that is an...

Controlled synthesis of open-mouthed epitope-imprinted polymer nanocapsules with a PEGylated nanocore and their application for fluorescence detection of target protein

“…Till now, epitope-imprinted materials have been utilized to recognize peptides, proteins and cells, and hence show potential applications in various areas, such as disease biomarker detection, recombinant protein purification, biosensors, cancer diagnosis and therapy. 16–25…”

“…1 The imprinting of proteins and other biomacromolecules is still challenged by their large size, complex structure and variable conformation. [2][3][4][5] Therefore, a variety of approaches, such as surface imprinting, [6][7][8][9][10] nanoscale imprinting, [11][12][13] solid synthesis 14,15 and epitope imprinting, [16][17][18][19][20] have been exploited to solve these problems.…”

“…Till now, epitope-imprinted materials have been utilized to recognize peptides, proteins and cells, and hence show potential applications in various areas, such as disease biomarker detection, recombinant protein purication, biosensors, cancer diagnosis and therapy. [16][17][18][19][20][21][22][23][24][25] For synthesis of epitope-imprinted polymers, surface imprinting with immobilized templates and sacricial the supports is an important way. It usually involves polymerization around the epitope templates orientedly immobilized on the surface of glass slides and other 2-D materials 26,27 or within the inner surface of macroporous silica spheres, [28][29][30][31][32] and hence the templates are fully encapsulated by the resultant polymers.…”

“…36,37 However, it is still a challenge for the synthesis of nano-sized imprinted materials with high surface-to-volume ratio by this approach, which are highly desired in the applications such as biosensors, separation and drug delivery. [16][17][18][19][20][21][22][23][24][25] As such, we recently put forward a method for synthesis of open-mouthed epitope-imprinted polymer nanocapsules (OM-MIP NCs), 38 with asymmetrically functionalized SiO 2 Janus nanoparticles (JNPs) as the supports for the formation of incomplete imprinted polymer shells and then fully etched using NH 4 HF 2 solution. The major surface of the JNPs is immobilized with both epitope templates and vinyl groups for subsequent surface imprinting via free radical polymerization (FRP), and the remaining surface graed with polyethleneglycol (PEG) chains for prevention from local polymerization.…”

Modulation of EGFR Activity by Molecularly Imprinted Polymer Nanoparticles Targeting Intracellular Epitopes

“…This method involved the synthesis of MIPs in the presence of whole protein, followed by partial proteolysis of the protein bound to polymer and subsequent sequencing of peptides bound to the polymer. We have previously shown the success of this approach with targets such as KRAS and acetylcholine esterase (AChE). , This technique has been modified for the characterization of surface proteins of whole cells, an approach which was dubbed “snapshot imprinting” . In this previous work, snapshot imprinting was performed for two cell lines, HN5 and MDA-MB-468, generating a list of epitopes with the potential to serve as good targets for nanoMIP binding.…”