Modulation of Adenosine Receptors and Antioxidative Effect of Beer Extracts in in Vitro Models

Alonso-Andrés, Patricia; Martı́n, Mairena; Albasanz, José Luís

doi:10.3390/nu11061258

Cited by 13 publications

(10 citation statements)

References 85 publications

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“…Chromatographic analysis was performed with Ultimate 3000U-HPLC, and data peaks were processed with Chromeleon 7 (ThermoFisher, Madrid, Spain) as previously described ( Alonso-Andres et al, 2019 ). An HPLC diode array was used working at 254 nm wavelength.…”

Section: Methodsmentioning

confidence: 99%

Antitumoral Action of Resveratrol Through Adenosinergic Signaling in C6 Glioma Cells

et al. 2021

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Gliomas are the most common and aggressive primary tumors in the central nervous system. The nucleoside adenosine is considered to be one major constituent within the tumor microenvironment. The adenosine level mainly depends on two enzymatic activities: 5′-nucleotidase (5′NT or CD73) that synthesizes adenosine from AMP, and adenosine deaminase (ADA) that converts adenosine into inosine. Adenosine activates specific G-protein coupled receptors named A1, A2A, A2B, and A3 receptors. Resveratrol, a natural polyphenol present in grapes, peanuts, and berries, shows several healthy effects, including protection against cardiovascular, endocrine, and neurodegenerative diseases and cancer. However, the molecular mechanisms of resveratrol actions are not well known. Recently, we demonstrated that resveratrol acts as an agonist for adenosine receptors in rat C6 glioma cells. The present work aimed to investigate the involvement of adenosine metabolism and adenosine receptors in the molecular mechanisms underlying the antitumoral action of resveratrol. Results presented herein show that resveratrol was able to decrease cell numbers and viability and to reduce CD73 and ADA activities, leading to the increase of extracellular adenosine levels. Some resveratrol effects were reduced by the blockade of A1 or A3 receptors by DPCPX or MRS1220, respectively. These results suggest that reduced CD73 activity located in the plasma membrane in addition to a fine-tuned modulatory role of adenosine receptors could be involved, at least in part, in the antiproliferative action of resveratrol in C6 glioma cells.

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Section: Methodsmentioning

confidence: 99%

Antitumoral Action of Resveratrol Through Adenosinergic Signaling in C6 Glioma Cells

et al. 2021

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“…Previous results of our group have demonstrated that beer extracts (lager, nonalcoholic, and dark beer) modulate adenosine receptors and protect from oxidative stress in cell cultures . We do not know which compounds are responsible for these effects, but we can point to common ingredients like hops.…”

Section: Introductionmentioning

confidence: 92%

“…45 In fact, both adenosine A 1 and A 2A receptor levels are increased after treatment with NH or CH, and this increase is similar to that which we reported previously when using beer extracts. 45 Interestingly, Nugget and Columbus hops are frequently used during brewing and could be responsible for the increase elicited by different beer extracts.…”

Section: Effect Of Xanthohumol In C6 and Sh-sy5y Cellmentioning

confidence: 99%

“…44 Previous results of our group have demonstrated that beer extracts (lager, nonalcoholic, and dark beer) modulate adenosine receptors and protect from oxidative stress in cell cultures. 45 We do not know which compounds are responsible for these effects, but we can point to common ingredients like hops. There are numerous varieties of hops and they are associated with beneficial properties, but there is a shortage of studies that elucidate the responsible molecule linked to its effects.…”

Section: ■ Introductionmentioning

confidence: 99%

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Modulation of Adenosine Receptors by Hops and Xanthohumol in Cell Cultures

Alonso

Albasanz

Martı́n

2021

ACS Chem. Neurosci.

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Adenosine receptors (ARs) have been involved in neurodegenerative diseases such as Alzheimer disease, where oxidative stress contributes to neurodegeneration and cell death. Therefore, there is increasing interest in developing antioxidative strategies to avoid or reduce neurodegeneration. We have previously described that different beer extracts modulate ARs and protect glioma and neuroblastoma cells from oxidative stress. The present work aimed to analyze the possible protective effect of hops (Humulus lupulus L.), a major component of beer, and xanthohumol on cell death elicited by oxidative stress and their modulation of ARs in rat C6 glioma and human SH-SY5Y neuroblastoma cells. Different extraction methods were employed in two hops varieties (Nugget and Columbus). Cell viability was determined by the XTT method in cells exposed to these hops extracts and xanthohumol. ARs were analyzed by radioligand binding and real-time PCR assays. Hops extract reverted the cell death observed under oxidative stress and modulated adenosine A 1 and A 2 receptors in both cell types. Xanthohumol was unable to revert the effect of oxidative stress in cell viability but it also modulated ARs similarly to hops. Therefore, healthy effects of beer described previously could be due, at least in part, to their content of hops and the modulation of ARs.

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“…Unfortunately, in this case, ethanol was not tested. Similarly, a total extract obtained from dark beer conferred higher protection to rat C6 glioma and human SH-SY5Y neuroblastoma cells against an oxidant stressor challenge (hydrogen peroxide) compared total extracts obtained from non-alcoholic and lager beers [ 91 ]. Again, neither the phenolic compounds of beers nor an alcoholic reconstituted extract were tested.…”

Section: Phenols-related Health Effects Of Beer Consumptionmentioning

confidence: 99%

The Role of Bioactive Phenolic Compounds on the Impact of Beer on Health

2021

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This review reports recent knowledge on the role of ingredients (barley, hop and yeasts), including genetic factors, on the final yield of phenolic compounds in beer, and how these molecules generally affect resulting beer attributes, focusing mainly on new attempts at the enrichment of beer phenols, with fruits or cereals other than barley. An entire section is dedicated to health-related effects, analyzing the degree up to which studies, investigating phenols-related health effects of beer, have appropriately considered the contribution of alcohol (pure or spirits) intake. For such purpose, we searched Scopus.com for any kind of experimental model (in vitro, animal, human observational or intervention) using beer and considering phenols. Overall, data reported so far support the existence of the somehow additive or synergistic effects of phenols and ethanol present in beer. However, findings are inconclusive and thus deserve further animal and human studies.

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Modulation of Adenosine Receptors and Antioxidative Effect of Beer Extracts in in Vitro Models

Cited by 13 publications

References 85 publications

Antitumoral Action of Resveratrol Through Adenosinergic Signaling in C6 Glioma Cells

Antitumoral Action of Resveratrol Through Adenosinergic Signaling in C6 Glioma Cells

Modulation of Adenosine Receptors by Hops and Xanthohumol in Cell Cultures

The Role of Bioactive Phenolic Compounds on the Impact of Beer on Health

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