Modulation of Akt-p38-MAPK/Nrf2/SIRT1 and NF-κB pathways by wine pomace product in hyperglycemic endothelial cell line

Gerardi, Gisela; Cavia-Sáiz, Mónica; Rivero-Pérez, María D.

doi:10.1016/j.jff.2019.05.003

Cited by 19 publications

(16 citation statements)

References 42 publications

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“…Reactive oxygen species (ROS) are produced by aerobic cells in the process of metabolism (Moloney & Cotter, ). Excessive accumulation of ROS can destroy the defense function of cells, leading to oxidative stress, DNA damage, and apoptosis, which is caused by the imbalance between antioxidants and oxidants (Gerardi, Cavia‐Saiz, Rivero‐Pérez, González‐Sanjosé, & Muñiz, ; Tang, Tan, Zhang, Dong, & Xu, ; Zhang & Wang, ). The occurrence of diseases such as endothelial dysfunction (Fratantonio et al, ), cancer (Poungpairoj et al, ), diabetes type II, neurodegenerative diseases (Gong et al, ), and cardiovascular diseases might be due to the overaccumulation of ROS (Alvarez‐Suarez et al, ; Wang, Wu, et al, ).…”

Section: Introductionmentioning

confidence: 99%

Protective effect of apple phlorizin on hydrogen peroxide‐induced cell damage in HepG2 cells

et al. 2019

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Apple phlorizin has many biological activities, such as antioxidant and liver protection. The present study aimed to evaluate the roles of apple phlorizin against hydrogen peroxide (H2O2)‐induced oxidative damage in HepG2 cells. In this study, treatment with apple phlorizin (100 and 150 μg/ml) decreased the production of reactive oxygen species and alleviated apoptosis as well as DNA damage in H2O2‐induced HepG2 cells. These effects were associated with the increased activity of antioxidant enzymes, enhanced the ARE‐driven phase II antioxidant gene expression and its upstream Nrf2 protein expression, and decreased apoptosis‐related gene expression. However, the phase II antioxidant gene expression and Nrf2 protein expression upregulated by phlorizin were reversed by Nrf2 shRNA transfection. These results showed that phlorizin relieves oxidative stress, DNA damage, and apoptosis in H2O2‐induced HepG2 cells, at least partially, by regulating the expression of Nrf2 protein and apoptosis‐related genes. Practical applications Apple phlorizin is a polyphenol compound extracted from apple or apple juice. This report highlighted a protective effect of phlorizin on antioxidant stress, DNA damage, and apoptosis in H2O2‐induced HepG2 cells. These results suggested that phlorizin may be developed for functional foods.

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Section: Introductionmentioning

confidence: 99%

Protective effect of apple phlorizin on hydrogen peroxide‐induced cell damage in HepG2 cells

et al. 2019

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“…It has been reported that oxalate can induce type II EMT by increasing reactive oxygen species (ROS) that act as secondary messenger to mediate type II EMT ( Convento et al., 2019 ; Rashed et al., 2004 ). In previous studies ( Gerardi et al., 2019 , 2020a ; Del Pino-García et al., 2016 , 2017 ), we demonstrated the in vitro and in vivo antioxidant activity of wine pomaces products by acting as direct scavengers of ROS, promoting the antioxidant systems and modulating signaling pathways of the redox cellular state such as Nrf2, NF-κB and SIRT1. Thus, the capacity of bioavailable WPPs fractions to reduce ROS levels and to modulate the Nrf2/NfkB pathway may explain the protective effect exerted by rWPP and wWPP fractions on oxalate-induced EMT ( Khand et al., 2002 ); differentiated MDCK cells therefore preserved epithelial morphology, peripheric E-cadherin and reduced vimentin fibers.…”

Section: Discussionmentioning

confidence: 81%

“…In previous studies (Gerardi et al, 2019;Del Pino-García et al, 2017;Gerardi et al, 2020a;b), we have demonstrated the cytoprotective role of polyphenols found in wine pomace products against pro-oxidant and pro-inflammatory agents.…”

Section: Introductionmentioning

confidence: 84%

“…The in vitro antioxidant capacity of each fraction (rWPGI, rWPF, wWPGI and wWPF) was determined previously ( Gerardi et al., 2019 , 2020c ) in the lyophilized powered fractions using QUENCHER (Q-) methods ( Del Pino-García et al., 2015 ). For the QUENCHER ABTS assay (Q-ABTS), 1 ± 0.005 mg of each fraction was mixed with 10 mL of the ABTS ∙+ working solution (ABTS 7 mM and potassium persulfate 2,45 mM in a ratio of 1:1 (v/v)).…”

Section: Methodsmentioning

confidence: 99%

“…The phenolic composition of each fraction was also evaluated previously by HPLC/DAD and GC/MS/MS ( Gerardi et al., 2019 , 2020c ). Identification and quantification of stilbenes, flavan-3-ols and flavonols was carried out using analytical reversed-phase HPLC on an Agilent 1100 series HPLC system (Agilent Technologies Inc., Palo Alto, CA, USA) coupled to a diode array detector and a Spherisorb3® ODS2 reversed phase C18 column (250 mm × 4.6 mm, 3 μm particle size; Waters Cromatografia S.A., Barcelona, Spain).…”

Section: Methodsmentioning

confidence: 99%

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Bioavailable wine pomace attenuates oxalate-induced type II epithelial mesenchymal transition and preserve the differentiated phenotype of renal MDCK cells

Gerardi

Casali

Cavia-Sáiz

et al. 2020

Heliyon

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The functional renal epithelium is composed of differentiated and polarized tubular cells with a strong actin cortex and specialized cell-cell junctions. If, under pathological conditions, these cells have to resist higher kidney osmolarity, they need to activate diverse mechanisms to survive external nephrotoxic agents such as inflammation and oxidative stress. Wine pomace polyphenols exert protective effects on renal cells. In this study, two winepomace products and their protective effects upon promotion and preservation of normal cell differentiation and attenuation of oxalate-induced type II epithelial mesenchymal transition (EMT) are evaluated. Treatment with gastrointestinal and colonic bioavailable fractions from red (rWPP) and white (wWPP) wine pomaces, both in the presence and the absence of oxalate, showed similar cell numbers and nuclear size than the non-treated differentiated MDCK cells. Immunofluorescence analysis showed the reduction of morphological changes and the preservation of cellular junctions for the rWPP and wWPP pre-treatment of cells exposed to oxalate injury. Hence, both rWPP and wWPP attenuated oxalate type II EMT in MDCK cells that conserved their epithelial morphology and cellular junctions through the antioxidant activities of grape pomace polyphenols.

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The implications of exercise in Drosophila melanogaster: insights into Akt/p38 MAPK/Nrf2 pathway associated with Hsp70 regulation in redox balance maintenance

et al. 2023

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Modulation of Akt-p38-MAPK/Nrf2/SIRT1 and NF-κB pathways by wine pomace product in hyperglycemic endothelial cell line

Cited by 19 publications

References 42 publications

Protective effect of apple phlorizin on hydrogen peroxide‐induced cell damage in HepG2 cells

Protective effect of apple phlorizin on hydrogen peroxide‐induced cell damage in HepG2 cells

Bioavailable wine pomace attenuates oxalate-induced type II epithelial mesenchymal transition and preserve the differentiated phenotype of renal MDCK cells

The implications of exercise in Drosophila melanogaster: insights into Akt/p38 MAPK/Nrf2 pathway associated with Hsp70 regulation in redox balance maintenance

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