Modulation of antibacterial activity of actinomycetes by co-culture with pathogenic bacteria

Haque, Uzzal; Rahman, Ajijur; Haque, Anwarul; Sarker, Ashish Kumar; Islam, Asiful

doi:10.3329/bpj.v18i1.23517

Cited by 4 publications

(2 citation statements)

References 16 publications

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“…This could be due to the ecological role such as defensive mechanism to maintain their niche, or invasion of an established microbial community [ 30 ]. It has been reported that live and heat-killed cells of S. aureus induced the production of secondary metabolites from marine microbial isolates [ 31 ]. About 119 microorganisms were screened and only 27 isolates were found active against one test organism [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Potential of Extract from a Pseudomonas aeruginosa Isolate

et al. 2022

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Microorganisms are one of the main sources of antimicrobial agents and over 50% of antibiotics currently used in hospitals are metabolites from microbes. This study aimed to isolate microorganisms from the Dompoase landfill site, Kwame Nkrumah University Physics Garden, Kosiko River, and Ada Foah seashore of Ghana and screen their metabolites for antimicrobial activity. Forty-eight (48) microorganisms were isolated and their metabolites were screened against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Vibrio cholerae, Salmonella typhi, Pseudomonas aeruginosa, Streptococcus pyogenes, Proteus mirabilis, and Candida albicans using the agar well diffusion method. Ten (10) of the isolates exhibited antimicrobial activity. Isolate DO5, identified as P. aeruginosa isolate, from the Dompoase landfill site was selected for fermentation because it exhibited the highest activity against all the test organisms. DO5 produced optimum antimicrobial activity when fermented for 11 days at 30°C. In the agar diffusion method, the extract of isolate DO5 recorded zones of inhibition ranging between 11.67 ± 0.23 and 21.50 ± 0.71 mm. The MIC and MBC recorded for the DO5 extract ranged from 3.13–25.0 mg/mL and from 6.25–50.0 mg/mL, respectively. Column chromatography analysis yielded eight (8) subfractions from the DO5 extract. IR analysis revealed the presence of functional groups such as alcohols, esters, and hydrocarbons in the fractions. GC-MS analysis identified nine compounds that have been reported to have antimicrobial agents. The DO5 metabolites stand the chance to be developed into potent antibiotics for infection treatment.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Potential of Extract from a Pseudomonas aeruginosa Isolate

et al. 2022

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“…"Further improvement of the system of continuing education, increasing the capacity of quality educational services, continuing the policy of training highly qualified personnel in accordance with the modern needs of the labor market" were identified as priorities in the field of medical education. In today's pandemic, the requirements for training doctors with modern knowledge, developing their professional competence, increasing their social activity are characterized by the above [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Technologies of methodical improvement of professional competence in the process of medical education

Musurmanova,

Kadirova,

Iminahunova

2023

BIO Web Conf.

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This article deals with issues of medical education, deontology of future doctors, methods of developing professional competence in medical education, comparative analysis of methodological support, pedagogical categories, professional qualities of medical staff, semantic content of medical education, factors influencing the health process, the importance of pedagogy in medical education. The tasks of education, distance learning in the process of medical education, the analysis of experimental results of research results are presented.

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Profiling bioactive compounds in secondary metabolites from co-cultivation between actinomycetes and pathogenic bacteria

Sari

Kusharyoto

Brazia

2020

IOP Conf. Ser.: Earth Environ. Sci.

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Co-cultivation is one of the ways to induce the discovery of new compounds by activating the cryptic genes in a microorganism’s genome. Actinomycetes is an order of bacteria with many clusters of gene coding for bioactive compounds that has not yet been expressed in the genome. In this study, co-cultivation between Actinomycetes strain DHE5-1 with pathogenic bacteria are conducted. Activity assay was done with disk diffusion method and the bioactive compounds in secondary metabolites are identified using HPLC UV-Vis. The results showed the activity of secondary metabolites from co-cultivation as an antimicrobial agent against the pathogenic microorganisms tested. The bioactive metabolite compounds showed different profiles produced between the mono and co-culture based on the models of the chromatogram. Finally, co-cultivation has the potential to induce the expression of covert genes in the organisms’ genome. This finding can promote the discovery of novel compounds with enhanced antimicrobial activity against resistant bacteria such as MDR-SA.

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Modulation of antibacterial activity of actinomycetes by co-culture with pathogenic bacteria

Cited by 4 publications

References 16 publications

Antimicrobial Potential of Extract from a Pseudomonas aeruginosa Isolate

Antimicrobial Potential of Extract from a Pseudomonas aeruginosa Isolate

Technologies of methodical improvement of professional competence in the process of medical education

Profiling bioactive compounds in secondary metabolites from co-cultivation between actinomycetes and pathogenic bacteria

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