Modulation of cathelicidins, IFNβ and TNFα by bovine alpha-herpesviruses is dependent on the stage of the infectious cycle

Burucúa, Mercedes; Quintana, Silvina; Lendez, Pamela Anahí; Cobo, Eduardo R.; Ceriani, María Carolina; Dolcini, Guillermina Laura; Odeón, Anselmo Carlos; Pérez, Sandra; Marin, Maia Solange

doi:10.1016/j.molimm.2019.04.022

Cited by 16 publications

(8 citation statements)

References 43 publications

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“…Antimicrobial peptides, particularly cathelicidins, as well as CD177 -mediated neutrophilic response in cattle, have been shown to be effective at mitigating Gram-negative bacterial infections and may be associated with modulating the cytotoxic/apoptotic host responses through the decrease of extracellular IL1A availability 61 , 64 , 67 . Dysregulation of these cytotoxic/apoptotic pathways is linked with poor clinical outcomes 60 , 68 , 69 . The increased cellular cornification/keratinization functions in treated_1 cattle versus healthy and treated_2+, identified through GO and pathway enrichment analyses, were impacted by increased expression of KRT5/10/14/25, DSG1, DSP, CDSN, and SPINK5 in this group.…”

Section: Discussionmentioning

confidence: 99%

Multipopulational transcriptome analysis of post-weaned beef cattle at arrival further validates candidate biomarkers for predicting clinical bovine respiratory disease

Scott

Woolums

Swiderski

et al. 2021

Sci Rep

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Bovine respiratory disease (BRD) remains the leading infectious disease in post-weaned beef cattle. The objective of this investigation was to contrast the at-arrival blood transcriptomes from cattle derived from two distinct populations that developed BRD in the 28 days following arrival versus cattle that did not. Forty-eight blood samples from two populations were selected for mRNA sequencing based on even distribution of development (n = 24) or lack of (n = 24) clinical BRD within 28 days following arrival; cattle which developed BRD were further stratified into BRD severity cohorts based on frequency of antimicrobial treatment: treated once (treated_1) or treated twice or more and/or died (treated_2+). Sequenced reads (~ 50 M/sample, 150 bp paired-end) were aligned to the ARS-UCD1.2 bovine genome assembly. One hundred and thirty-two unique differentially expressed genes (DEGs) were identified between groups stratified by disease severity (healthy, n = 24; treated_1, n = 13; treated_2+, n = 11) with edgeR (FDR ≤ 0.05). Differentially expressed genes in treated_1 relative to both healthy and treated_2+ were predicted to increase neutrophil activation, cellular cornification/keratinization, and antimicrobial peptide production. Differentially expressed genes in treated_2+ relative to both healthy and treated_1 were predicted to increase alternative complement activation, decrease leukocyte activity, and increase nitric oxide production. Receiver operating characteristic (ROC) curves generated from expression data for six DEGs identified in our current and previous studies (MARCO, CFB, MCF2L, ALOX15, LOC100335828 (aka CD200R1), and SLC18A2) demonstrated good-to-excellent (AUC: 0.800–0.899; ≥ 0.900) predictability for classifying disease occurrence and severity. This investigation identifies candidate biomarkers and functional mechanisms in at arrival blood that predicted development and severity of BRD.

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Section: Discussionmentioning

confidence: 99%

Multipopulational transcriptome analysis of post-weaned beef cattle at arrival further validates candidate biomarkers for predicting clinical bovine respiratory disease

Scott

Woolums

Swiderski

et al. 2021

Sci Rep

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“…61 The CAMP level has been found to increase due to respiratory tract infection in cattle. 62 Similarly, CAMP levels were found to increase in cattle with mastitis. 63 The upregulation of toll-like receptors (TLR's) 3, 7 and 9 and the activation of immune mechanisms are presented as the reason for the increase.…”

Section: Discussionmentioning

confidence: 93%

“…In a human subject research, CAMP provided in vitro protection against herpes simplex virus type 1 61 . The CAMP level has been found to increase due to respiratory tract infection in cattle 62 . Similarly, CAMP levels were found to increase in cattle with mastitis 63 .…”

Section: Discussionmentioning

confidence: 96%

Investigation of haematological, inflammatory and immunological response in naturally infected cattle with Theileria annulata

Aktaş

Eren

Küçükler

et al. 2023

Parasite Immunology

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In this study, we aimed to investigate haematological, pro‐inflammatory, inflammatory, anti‐inflammatory and immunological responses in naturally Theileria annulata‐infected cattle. The study material consisted of 25 Simmental cattle, 2–4 years of age, one of which was a control group consisting of healthy animals (Control group, n = 10), and the other was a Theileria group that include animals positive for Theileria annulata (Theileria group, n = 15). Haematological analysis (red blood cell [RBC], haemoglobin [HGB], haematocrit [HCT]), pro‐inflammatory (tumour necrosis factor‐α [TNF‐α], nuclear factor kappa B [NF‐ĸB] and interleukin‐1 beta, [IL‐1β]), inflammatory (neutrophil‐lymphocyte ratio [NLR]), anti‐inflammatory (interleukin‐10 [IL‐10]) and antimicrobial peptide (CAMP) analyses were performed by using ELISA kit from blood samples. It was found that the rectal temperature of the Theileria group was found to be significantly higher (p < .001) than that of the control group. Haematological and biochemical analysis revealed that the RBC and HGB count and HCT percentage decreased (p < .001), while NF‐ĸB (p < .001), TNF‐α (p = .002), IL‐1β (p < .001), IL‐10 (p = .012), NLR (p < .001) and CAMP (p = .037) levels increased in Theileria group compared to the control group. There was a strong correlation between NF‐ĸB and TNF‐α, NF‐ĸB and IL‐10, NLR and IL‐1β, NF‐ĸB and CAMP, TNF‐α and CAMP and IL‐10 and CAMP. As a result of this study, it was revealed that a pro‐inflammatory and immunological response also occurs along with the anti‐inflammatory response in the inflammatory process.

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“…Thus, establishment of latency was confirmed. The results of these experimental inoculations have been previously published [16–20].…”

Section: Methodsmentioning

confidence: 99%

Differential expression of cyclins mRNA in neural tissues of BoHV-1- and BoHV-5- infected cattle

Marin

Burucúa

Rensetti

et al. 2019

Preprint

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26Introduction 27 Bovine alphaherpesvirus types 1 (BoHV-1) and 5 (BoHV-5) are closely related 28 alphaherpesviruses. BoHV-5 causes non-suppurative meningoencephalitis in calves. 29 BoHV-1 is associated with several syndromes and, occasionally, can cause encephalitis. 30 Although both viruses are neurotropic and they share similar biological properties, it is 31 unknown why these alphaherpesviruses differ in their ability to cause neurological disease. 32 Materials and Methods 33Neural tissue samples were collected from BoHV-1-and BoHV-5-intranasally inoculated 34 calves during acute infection, latency and reactivation. The levels of cyclins mRNA 35 expression in neural tissue from calves infected with BoHV-1 or BoHV-5 were analyzed by 36 qRT-PCR. Data were analyzed by Relative Expression Software Tool (REST). 37 Results 38 Striking differences in the levels of cyclins mRNA were observed between uninfected and 39 infected tissues, particularly in trigeminal ganglion (TG). During acute infection, higher 40 levels of cyclin A2, E1 and B1 were observed in BoHV-1 and BoHV-5-infected TG 41 compared with uninfected TG. mRNA levels of cyclins A2 and E1 were downregulated in 42 olfactory cortex. During latent infection with BoHV-1 and BoHV-5, cyclin A2 and E1 were 43 downregulated in olfactory cortex and cervical medulla whereas cyclin B1 was upregulated 44 in BoHV-1-infected olfactory and frontal cortex and in cervical medulla after BoHV-5 45 infection. A marked increase of cyclins A2 and E1mRNA levels was detected in TG of 3 46 BoHV-5-latently-infected cattle. Unlike in uninfected TG, in BoHV-1 and BoHV-5-47 infected TG, cyclin B1expression was detectable. During reactivation, the levels of cyclin 48 A2, B1 and E1 mRNA increased in TG. The expression levels of cyclins in TG during 49BoHV-5 latency suggest that these viruses utilize different strategies to persist in the host. 50 Conclusion 51Bovine alphaherpesviruses neuropathogenicity might be influenced by the differential 52 control of cell cycle components by these herpesviruses. This is the first report on BoHV-5 53 modulation of cyclins expression in neural tissues from its natural host. 54 55 56 57 58 59 60 61 62 63 64 65 4 66 67 127 Carlsbad, CA, USA) according to the manufacturer's protocol and digested with DNase I 128 Amplification Grade (Invitrogen, Carlsbad, CA, USA). Complementary DNA (cDNA) was 129 synthesized and negative controls, omitting the RNA or the reverse transcriptase, were 130 included. Real-time RT-PCR reactions for cyclins A2, B1 and E1 were performed using 131 800 nM specific primers (Table 1), 1X PCR Master Mix (FastStart Universal SYBR Green 132 Master Rox, Roche, Mannhein, Germany) and 1 µl of cDNA in a final volume of 20 µl.133 7 134 135 157 These differences were particularly evident in TG at all stages of the infectious cycle. 158 Cyclin B1 was undetected in TG of uninfected calves. During acute infection, cyclin A2 159 and E1, were significantly higher (p < 0.05) in BoHV-1 and BoHV-5-infected TG 160 compared with uninfected T...

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Modulation of cathelicidins, IFNβ and TNFα by bovine alpha-herpesviruses is dependent on the stage of the infectious cycle

Cited by 16 publications

References 43 publications

Multipopulational transcriptome analysis of post-weaned beef cattle at arrival further validates candidate biomarkers for predicting clinical bovine respiratory disease

Multipopulational transcriptome analysis of post-weaned beef cattle at arrival further validates candidate biomarkers for predicting clinical bovine respiratory disease

Investigation of haematological, inflammatory and immunological response in naturally infected cattle with Theileria annulata

Differential expression of cyclins mRNA in neural tissues of BoHV-1- and BoHV-5- infected cattle

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