Modulation of eicosanoid-induced contraction of mouse and rat blood vessels by gingerols.

Abstract

Abstract-The effects of the active principles of crude ginger (a traditional Sino Japanese medicine), the gingerols, on the contractile responses to eicosanoids were compared using isolated mouse and rat blood vessels. Leukotrienes (LT) C4 and D4, a thromboxane (TX) A2 derivative (U-46619), prostaglandins (PG) F2a, PG12-Na, PGE2, the stable PG12 derivative TRK-100, and PGD2 induced con traction in longitudinal segments of mouse mesenteric veins in that order of potency. Exogenous arachidonic acid and PGE, did not cause contraction. The mesenteric veins also contracted in response to noradrenaline (NA) and phenylephrine (PhE), but not to clonidine. The gingerols alone relaxed the muscle transiently and then augmented the response to PGF2a, PGE2, PG12-Na, and TRK-100, but suppressed the response to PGD2, U-46619, LTC4, LTD4, NA and PhE. (±)-[6]-Gingerol also potentiated the PGF2a-induced contraction in longitudinal segments of rat mesen teric vein and vena cava, but inhibited it in circular segments of rat aorta and longi tudinal segments of mouse mesenteric arteries. These results showed that (±)

Diabetes-induced enhancement of prostanoid-stimulated contraction in mesenteric veins of mice.

“…This demonstrates the presence of receptors for each eicosanoid in the venous smooth muscle (24). The in fluence of diabetes on the synthesis of prostanoids has been reported, suggesting decreased prostacyclin production and in creased TX synthesis (10,25).…”

6‐Gingerol prevents cisplatin‐induced acute renal failure in rats

“…It is possible that the polyphenol gingerol had induced phase-II detoxifying and antioxidant enzymes via activation of Keap1-Nrf2-ARE. Previous studies showed that (+/−)- [5]-gingerols inhibited the contractions in isolated rat blood vessels, induced by prostanoids PGD-2, TXA-2, and LT, suggesting the modulation of eicosanoids-induced responses by these compounds also [29]. Previous studies showed that (+/−)- [5]-gingerols inhibited the contractions in isolated rat blood vessels, induced by prostanoids PGD-2, TXA-2, and LT, suggesting the modulation of eicosanoids-induced responses by these compounds also [29].…”

Gingerol Inhibits Serum-Induced Vascular Smooth Muscle Cell Proliferation and Injury-Induced Neointimal Hyperplasia by Suppressing p38 MAPK Activation

“…23 To assess the per se effect of gingerol, rat aortic rings were incubated with a wide range of concentrations of gingerol (30-300 mmol/L), which was based on the previous published reports. 24,25 Gingerol was incubated for 30 minutes and dose-response of PE (1-100 mmol/L) was monitored. To assess the per se effect of gingerol (3-300 mmol/L) for their vasorelaxant efficacy, responses were obtained in PE (1 mmol/L) precontracted endothelium intact rings.…”