Modulation of Gastrointestinal Bacterial in Chronic Atrophic Gastritis Model Rats by Chinese and West Medicine Intervention

Abstract

Chronic atrophic gastritis (CAG) was well-known related with multiple pathogenic factors and normally therapies comprised by western or Chinese medicines. Present study was design to identified the bacterial community characterized by 16S rRNA amplicon sequencing and determine the modulate affection of bacterial composition response western and Chinese medicine Qing huayin (QHY) as well as antibiotic on model rats. Result shown the overall structure alteration of bacterial appeared under medicine applied, antibiotic caused a marked depletion in bacterial diversity and richness, the enrichment of Firmicutes (85.1-90.7%) in antibiotic-free convert to Bacteroidetes (30.7-34.6%) in antibiotic-added model rat were demonstrated. Firmicutes was most dominant phylum and accounting for 85.1%-90.5% and significantly decreased till 21.9%-68.5% in antibiotic-added treatments. Especially QHY-treated show highest RA of Firmicutes (90.5%) and the amelioration of CAG using QHY attributed by beneficial bacterial enrichment, especially Ruminococcus, Lactobacillus and Bifidobacterium. In addition, alpha and beta diversity analysis also demonstrated the clear dispersion and aggregation that revealed the alteration and steady of bacterial community structures. In summary, QHY has potential application value in the treatment of CAG which attributed to close relation with the modulatory of internal bacterial communities.