Modulation of Gut Microbiota and Oxidative Status by β-Carotene in Late Pregnant Sows

Yuan, Xupeng; Yan, Jiahao; Hu, Ruizhi; Li, Yanli; Wang, Ying; Chen, Hui; Hou, De‐Xing; He, Jianhua; Wu, Shusong

doi:10.3389/fnut.2020.612875

Cited by 23 publications

(18 citation statements)

References 37 publications

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“…Multiple studies have focused on the effect of β-carotene on offspring, however, the impact of β-carotene on maternal and postpartum recovery is not clear. Our recent study has shown that dietary supplementation of β-carotene had a positive effect on the reproductive performance of sows ( 8 ), and in vitro inhibitory effect of β-carotene on cytokines was revealed by Lin et al. ( 25 ).…”

Section: Discussionmentioning

confidence: 97%

“…Gut microbiota was characterized by 16S rRNA gene sequencing as described previously ( 8 ). Briefly, Total DNA was extracted from cecum contents by using a DNA Isolation Kit (MoBio Laboratories, Carlsbad, CA, USA) following the manufacturer’ s manual.…”

Section: Methodsmentioning

confidence: 99%

“…β-carotene metabolism plays an important role during mammalian embryonic development ( 6 ). Recent studies have suggested that dietary β-carotene supplementation during late pregnancy has benefit to the birth weight of offspring in sows ( 7 , 8 ), and injection of β-carotene can increase the litter size ( 9 ). Moreover, β-carotene has been reported to suppress inflammatory responses in macrophages by inhibiting NF-κB translocation ( 10 ), which can induce the expression of pro-inflammatory genes ( 11 ) and promote inflammatory diseases ( 12 ).…”

Section: Introductionmentioning

confidence: 99%

“…Gut microbiota can influence intestinal permeability, thereby promoting translocation of bacterial products to induce inflammation ( 20 ), which occurs throughout pregnancy ( 21 ). Our previous studies have also revealed that gut microbiota is closely correlated with inflammation and intestinal mucosal barrier function ( 22 ), and dietary supplementation of β-carotene can regulate host gut microbiota ( 8 ).…”

Section: Introductionmentioning

confidence: 99%

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Dietary β-Carotene on Postpartum Uterine Recovery in Mice: Crosstalk Between Gut Microbiota and Inflammation

Yang

et al. 2021

Front. Immunol.

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As the precursor of vitamin A, β-carotene has a positive effect on reproductive performance. Our previous study has shown that β-carotene can increase antioxidant enzyme activity potentially through regulating gut microbiota in pregnant sows. This study aimed to clarify the effect of β-carotene on reproductive performance and postpartum uterine recovery from the aspect of inflammation and gut microbiota by using a mouse model. Twenty-seven 6 weeks old female Kunming mice were randomly assigned into 3 groups (n=9), and fed with a diet containing 0, 30 or 90 mg/kg β-carotene, respectively. The results showed that dietary supplementation of β-carotene reduced postpartum uterine hyperemia and uterine mass index (P<0.05), improved intestinal villus height and villus height to crypt depth ratio, decreased serum TNF-α and IL-4 concentration (P<0.05), while no differences were observed in litter size and litter weight among three treatments. Characterization of gut microbiota revealed that β-carotene up-regulated the relative abundance of genera Akkermansia, Candidatus Stoquefichus and Faecalibaculum, but down-regulated the relative abundance of Alloprevotella and Helicobacter. Correlation analysis revealed that Akkermansia was negatively correlated with the IL-4 concentration, while Candidatus Stoquefichus and Faecalibaculum had a negative linear correlation with both TNF-α and IL-4 concentration. On the other hand, Alloprevotella was positively correlated with the TNF-α, and Helicobacter had a positive correlation with both TNF-α and IL-4 concentration. These data demonstrated that dietary supplementation of β-carotene contributes to postpartum uterine recovery by decreasing postpartum uterine hemorrhage and inhibiting the production of inflammatory cytokines potentially through modulating gut microbiota.

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Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dietary β-Carotene on Postpartum Uterine Recovery in Mice: Crosstalk Between Gut Microbiota and Inflammation

Yang

et al. 2021

Front. Immunol.

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“…The results suggested that the β-carotene lower the composition of Firmicutes such as Lachnospiraceae AC2044 group, Lachnospiraceae NK4B4 group, as well as Ruminococcaceae UCG-008, but on the other hand significantly enriched the Proteobacteria and Actinobacteria having related to NO synthesis. It was concluded that the supplementation of dietary β-carotene might rise antioxidant enzyme activity and NO which is an important to promote the neonatal blood circulation, through regulating gut microbiota in sows [169].…”

Section: Fat-soluble Vitaminsmentioning

confidence: 99%

Role of Food Antioxidants in Modulating Gut Microbial Communities: Novel Understandings in Intestinal Oxidative Stress Damage and Their Impact on Host Health

et al. 2021

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Dietary components have an important role on the structure and function of host gut microbial communities. Even though, various dietary components, such as carbohydrates, fats, proteins, fibers, and vitamins, have been studied in depth for their effect on gut microbiomes, little attention has been paid regarding the impact of several food antioxidants on the gut microbiome. The long-term exposure to reactive oxygen species (ROS) can cause microbial dysbiosis which leads to numerous intestinal diseases such as microbiota dysbiosis, intestinal injury, colorectal cancers, enteric infections, and inflammatory bowel diseases. Recently, it has been shown that the food derived antioxidant compounds might protect the host from intestinal oxidative stress via modulating the composition of beneficial microbial species in the gut. The present review summarizes the impact of food antioxidants including antioxidant vitamins, dietary polyphenols, carotenoids, and bioactive peptides on the structure as well as function of host gut microbial communities. Several in vitro, animal model, and clinical studies indicates that food antioxidants might modify the host gut microbial communities and their health status. However, still further clarification is needed as to whether changes in certain microbial species caused by food additives may lead to changes in metabolism and immune function.

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Dietary ferulic acid and vanillic acid on inflammation, gut barrier function and growth performance in lipopolysaccharide-challenged piglets

et al. 2022

Animal Nutrition

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Ferulic acid (FA) and vanillic acid (VA) are considered as major phenolic metabolites of cyanidin 3-glucoside, a polyphenol that widely exists in plants that possess a protective effect against oxidative stress and inflammation in our previous study. This study aimed to investigate the effect of FA and VA on inflammation, gut barrier function, and growth performance in a weaned piglet model challenged with lipopolysaccharide (LPS). Thirty-six piglets (PIC 337 × C48, 28 d of age) were randomly allocated into 3 treatments with 6 replicate pens (2 piglets per pen). They were fed with a basal diet or a diet containing 4,000 mg/kg of FA or VA. Dietary supplementation of VA significantly increased average daily gain (ADG) (P < 0.05). Both FA and VA decreased serum levels of thiobarbituric acid reactive substances (TBARS), interlukin (IL)-1β, IL-2, IL-6, and tumor necrosis factor (TNF)-α ( P < 0.05), and enhanced the expression of tight junction protein oclaudin ( P < 0.05). Analysis of gut microbiota indicated that both FA and VA increased the Firmicutes/Bacteroidetes ratio alongside reducing the relative abundance of the Prevotellaceae family including Prevotella 9 and Prevotella 2 genera, but enriched the Lachoiraceaea family including the Lachnospiraceae FCS020 group ( P < 0.05). Moreover, VA reduced the relative abundance of Prevotella 7 and Prevotella 1 but enriched Lachnospira , Eubacterium eligens group, and Eubacterium xylanophilum group ( P < 0.05), while FA showed a limited effect on these genera. The results demonstrated that both VA and FA could alleviate inflammation and oxidative stress, but only VA has a significant positive effect on the growth performance of LPS-challenged piglets potentially through modulating gut microbiota.

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Modulation of Gut Microbiota and Oxidative Status by β-Carotene in Late Pregnant Sows

Cited by 23 publications

References 37 publications

Dietary β-Carotene on Postpartum Uterine Recovery in Mice: Crosstalk Between Gut Microbiota and Inflammation

Dietary β-Carotene on Postpartum Uterine Recovery in Mice: Crosstalk Between Gut Microbiota and Inflammation

Role of Food Antioxidants in Modulating Gut Microbial Communities: Novel Understandings in Intestinal Oxidative Stress Damage and Their Impact on Host Health

Dietary ferulic acid and vanillic acid on inflammation, gut barrier function and growth performance in lipopolysaccharide-challenged piglets

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