Modulation of immunological responses by aqueous extract of Datura stramonium L. seeds on cyclophosphamide-induced immunosuppression in Wistar rats

Abstract

Background Datura stramonium L. (Solanaceae) is used traditionally in west Africa to treat asthma, epilepsy, rheumatoid arthritis, filariasis microbial infections and conjunctivitis. This study investigated the immunomodulatory effects of aqueous seed extract of D. stramonium L. (ASEDS) on Wistar rats. Methods Thirty Wistar albino rats (180–200 g) were randomized into 6 groups (n = 5). Group 1 received distilled water only. Rats in groups 2–6 were pretreated with 10 mg/kg body weight (b.w.) Cyclophosphamide orally for 27-days to induce immunosuppression. Thereafter, they received treatment orally for 28 days as follows: Group 2 (distilled water), group 3 (5 mg/kg b.w. Levamisole), groups 4–6 (60, 90 and 120 mg/kg b.w. ASEDS, respectively). HPLC was used to determine major compounds in ASEDS. The effects of ASEDS on immune cells, immunoglobulins A, G and M levels, lipoproteins, and antioxidant status of rats were evaluated. Results ASEDS indicated high content of Acutumine, Quinine, Catechin, Chlorogenic acid, Gallic acid, Quercetin, Vanillic acid, Luteolin, Formosanin C, Saponin, Cyanidin, Tannic acid, 3-Carene, Limonene and α-terpineol. Cyclophosphamide triggered significant (p < 0.05) reduction in total leucocyte count and differentials, IgA, IgG, high-density lipoproteins (HDL), catalase, superoxide dismutase, glutathione peroxidase, vitamins A, C and E levels of untreated rats. Administration of ASEDS led to significant (p < 0.05) improvement in immune cell counts, immunoglobulin synthesis, high-density lipoprotein concentration, and antioxidant status of rats in the treated groups. Conclusions The results obtained from the study showed the immunomodulatory activity of ASEDS, thereby indicating its potential in immunostimulatory drug discovery.

Reprogramming of arachidonic acid metabolism using α-terpineol to alleviate asthma: insights from metabolomics

“…Previous studies have shown that α-terpineol alleviates asthma in mice by reducing EOS and balancing Th1/Th2 cytokines. 31–33 In this study, we also found that α-terpineol decreased the production of the Th2-type cytokines IL-4, IL-5 and IL-13. 26,32,34 The molecular mechanism by which α-terpineol exerts protective effects against asthma was analysed using UPLC/MS-MS techniques.…”

“…Previous studies have shown that α-terpineol alleviates asthma in mice by reducing EOS and balancing Th1/Th2 cytokines. [31][32][33] In this study, we also found that α-terpineol decreased the production of the Th2-type cytokines IL-4, IL-5…”

Mechanistic role of quercetin as inhibitor for adenosine deaminase enzyme in rheumatoid arthritis: systematic review

“…This inhibitory effect is dieted by reduced the adenosine elevated levels through restoration of T-cell homeostasis [ 125 – 128 ], regulation of Th17 cell differentiation [ 129 ], regulation of Th17/Treg-related cytokine levels, reduction of autoantibody production, and regulation of nucleoside triphosphate diphosphohydrolase (NTPDase) activities [ 128 , 130 ]. Collectively, QUE established the immune-regulatory effect and is considered one of the most important natural candidates that can used for RA therapy [ 131 , 132 ] as mentioned in Fig. 11 .…”

Osteogenic mechanism of chlorogenic acid and its application in clinical practice

“…Pathological examination showed arthritic cell infiltration and cartilage erosion in the model group, and a slight relief of joint lesions was observed in the CGA group. Therefore, CGA is also gradually being used to treat RA ( Joshua et al, 2022 ; Lee et al, 2018 ). Lee et al examined the effect of CGA on septic arthritis caused by Candida albicans and found a significant reduction in edema ( Lee et al, 2008 ).…”