Modulation of intestinal mucosal immunity by dietary polyunsaturated fatty acids in chickens

Abstract

This study was undertaken to investigate the effects of dietary polyunsaturated fatty acids (PUFA) on intestinal immune responses and cytokines secretions in chickens at nutritionimmunity level. The proportion of CD3' CD8 ' lymphocytes of intestinal mucosa in CO group was higher on d 21 than that in FO (p B/ 0.01) and PO (p B/ 0.05) group. The proportion of CD3' CD4 ' lymphocytes of FO group was greatly significantly higher on d 21, relative to PO or CO group. The FO treatment enhanced the sIgA concentration in jejunum on d 42, compared with CO and PO group. Fish oil and corn oil had similar effects on enhancing IL-2 secretion of PBMC, and converse effects on IL-4 secretion. Compared with PO group, chickens supplemented with fish oil or corn oil had greater concentration of intestinal mucosal membrane phospholipids on d 21 and d 42. In conclusion, the changes of intestinal lymphocytes membrane phospholipids in FO or CO group would modulate lipid second messengers. The changes of CD expression would affect sIgA secretion of intestinal lymphocytes. Diet supplemented with corn oil or fish oil can affect cytokines secretion of PBMC in chickens, which works on systemic and mucosal immune response.

FATTY ACIDS PROFILE OF MEAT, MUCOSAL sIgA CONCENTRATION AND PRODUCTION INDEX OF BROILER AS A RESPONSE TO CHLORELLA sp. ADMINISTRATION IN THE DIET

“…The production of sIgA is of major importance to the function of the mucosal immune systems of broiler. Like the other type of immunoglobulins, the mucosal sIgA concentration of poultry is affected by the ratio of n-3 to n-6 PUFA through eicosanoid mediated effect (Miura et al, 1998;Yang and Guo, 2006). In accordance to Spolaore et al (2006) who have shown that alga as a part of animal feed could improve the immune response, and also concomitant to Yang and Guo (2006), our results suggest that increased levels of Chlorella sp.…”

Effect of dietary inulin supplements on growth performance and intestinal immunological parameters of broiler chickens

“…The lymphocyte subpopulations (CD3 þ , CD4 þ and CD8 þ T cells) in the lymphocyte cell suspension prepared above were determined using the flow cytometric method (Holt et al, 2010;Yang and Guo, 2006). Viable cell counts were determined by trypan blue exclusion and the cell concentrations were adjusted to 2 Â 10 6 viable cells/ml.…”

“…Briefly, the mesentery-, fat-and blood vessels-free ileum segments were cut into o0.5 cm pieces, washed with PBS containing 100 IU/ml penicillin and 100 μg/ml streptomycin and incubated at 37°C with 20 ml digestive culture medium (Yang and Guo, 2006) for 90 min. The suspensions were filtered through 200 mm nylon cloth and the filtrates were centrifuged with an Allegra™ 64R Centrifuge (Beckman Coulter, Inc., CA) at 500g for 10 min.…”

Can breeder reproductive status, performance and egg quality be enhanced by supplementation and transition of n‐3 fatty acids?

“…However, given the inconsistent results of several studies, this concern may or may not be justified. Some studies show no effect (Puthpongsiriporn and Scheideler, , Bautista‐Ortega et al., ), some show a detrimental effect (Pappas et al., ; Al‐Khalifa et al., ), and some show an improvement (Wang et al., ; Yang and Guo, ; Enting et al., ). In our study, the inclusion level of 3.0% was probably too high, thereby provoking negative effects.…”