Modulation of keratin in adhesion, proliferation, adipogenic, and osteogenic differentiation of porcine adipose‐derived stem cells

Abstract

Recently, keratin attracts tremendous interest because of its intrinsic ability to interact with different cells. It has the potential to serve as a controllable extracellular matrix protein that can be used to demonstrate cell mechanism and cell-matrix interaction. However, there have been relatively few studies on the effects of keratin on stem cells. In the present work, we study the effects of human keratin on porcine adipose-derived stem cells (pASCs) and a series of selective cell lines: 3T3 fibroblasts, Madin-Darby canine kidney (MDCK) cells, and MG63 osteoblasts. Relative to un-treated culture plate, our results showed that keratin coating substrates promote cell adhesion and proliferation to above cell lines. Keratin also improved pASCs adhesion, proliferation, and enhanced cell viability. Evaluation of genetic markers showed that adipogenic and osteogenic differentiations of pASCs can be successfully induced, thus demonstrating that keratin did not influence the stemness of pASCs. Furthermore, keratin improved adipogenic differentiations of pASCs in terms of up-regulations in lipoprotein lipase, peroxisome proliferator-activated receptor gamma, and CCAAT-enhancer-binding protein alpha. The osteogenic markers type I collagen, runt-related transcription factor 2, and vitamin D receptor were also upregulated when pASCs cultured on keratin substrates. Therefore, keratin can serve as a biological derived material for surface modification and scaffold fabrication for biomedical purpose. The combination of keratin with stem cells may be a potential candidate for tissue repair in the field of regenerative medicine. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 180-192, 2017.

Keratin scaffolds with human adipose stem cells: Physical and biological effects toward wound healing

“…Overall, the results of real-time PCR corroborated the adipogenic and osteogenic differentiation potential of hASCs in keratin scaffolds. The results were consistent with our previous study, showing increasing PPAR-γ and RUNX2 gene expression activities on keratin coating (Wu et al, 2017).…”

“…Keratin was prepared from human hair according to the method reported previously (Nakamura, Arimoto, Takeuchi, & Fujii, 2002;Wu, Lin, Cheng, Yang, & Yu, 2017). First, human hair was washed with distilled water three times and then soaked in 300 ml mixture of methanol/chloroform (2:1, v/v; ECHO Chemical Co., Taiwan) for 18-24 hr to remove lipid covering the external part of the hair.…”

The Overexpression of Tβ4 in the Hair Follicle Tissue of Alpas Cashmere Goats Increases Cashmere Yield and Promotes Hair Follicle Development

“…Five candidate genes were identified by both the transcriptomic and proteomic analyses, and two of these (KRT2 and KRT4) were shown to have similar expression patterns by k-means clustering. As KRT homologs are closely related to hair growth [43][44][45], we separately overexpressed KRT2 and KRT4 in SHF-DPCs and found that KRT4 increased the growth of SHF-DPCs. In addition, our investigation of proliferation-related signaling pathways showed that the ERK signaling pathway was significantly enhanced in Tβ4+ goats, which is consistent with previous reports that this signaling pathway is involved in the regulation of hair follicle development in a variety of model animals [41,46,47].…”

Electrospun PCL/keratin/AuNPs mats with the catalytic generation of nitric oxide for potential of vascular tissue engineering

“…Keratins have outstanding cytocompatibility due to their cell adhesive Arg–Gly–Asp (RGD) and Leu–Asp–Val (LDV) sequences . Recently, Yu group demonstrated that keratins could improve adhesion, proliferation, and viability of porcine adipose‐derived stem cells . Metal ions (e.g., Ag + and Au 3+ ) can be easily bound under the chelating effect and electrostatic attraction by virtue of the abundant free amino and carboxyl groups of keratins .…”

“…8 Recently, Yu group demonstrated that keratins could improve adhesion, proliferation, and viability of porcine adipose-derived stem cells. 9 Metal ions (e.g., Ag + and Au 3+ ) can be easily bound under the chelating effect and electrostatic attraction by virtue of the abundant free amino and carboxyl groups of keratins. [10][11][12] In addition, the formed metal nanoparticles are stable due to the chemical gold-sulfur (Au-S) or silversulfur (Ag-S) bonds.…”