Modulation of LPS-induced ROS production and NF-?B nuclear translocation by N-stearoylethanolamine in macrophages

Berdyshev, A. G.; Kosiakova, H. V.; Hula, N M

doi:10.15407/ubj89.05.062

Cited by 4 publications

(4 citation statements)

References 21 publications

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“…But in our study, we have shown that the activation of NF-κB by LPS-induced TLR4/MyD88-accelerated ROS production is collectively LC8 and IkB-a dependent. Some reports, utilizing LPS as a ROS inducer, support our results ( 74 , 75 ). The results of our study with a little modification from those of Cario ( 76 ) and Fukata et al.…”

Section: Discussionsupporting

confidence: 90%

Pasture intake protects against commercial diet-induced lipopolysaccharide production facilitated by gut microbiota through activating intestinal alkaline phosphatase enzyme in meat geese

Ali

Farooq

et al. 2022

Front. Immunol.

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IntroductionDiet strongly affects gut microbiota composition, and gut bacteria can influence the intestinal barrier functions and systemic inflammation through metabolic endotoxemia. In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production. The effects of artificial pasture grazing system (AGF, a high dietary fiber source) on modulating gut microbiota architecture and gut barrier functions have not been investigated in meat geese. Therefore, this study aimed to investigate whether intestinal ALP could play a critical role in attenuating reactive oxygen species (ROS) generation and ROS facilitating NF-κB pathway-induced systemic inflammation in meat geese.MethodsThe impacts of IHF and AGF systems on gut microbial composition via 16 sRNA sequencing were assessed in meat geese. The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.Results and DiscussionIn the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system. A significant increase in intestinal ALP-induced Nrf2 signaling pathway was confirmed representing LPS dephosphorylation mediated TLR4/MyD88 induced ROS reduction mechanisms in AGF meat geese. Further, the correlation analysis of top 44 host markers with gut microbiota showed that artificial pasture intake protected gut barrier functions via reducing ROS-mediated NF-κB pathway-induced gut permeability, systemic inflammation, and aging phenotypes. In conclusion, the intestinal ALP functions to regulate gut microbial homeostasis and barrier function appear to inhibit pro-inflammatory cytokines by reducing LPS-induced ROS production in AGF meat geese. The AGF system may represent a novel therapy to counteract the chronic inflammatory state leading to low dietary fiber-related diseases in animals.

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Section: Discussionsupporting

confidence: 90%

Pasture intake protects against commercial diet-induced lipopolysaccharide production facilitated by gut microbiota through activating intestinal alkaline phosphatase enzyme in meat geese

Ali

Farooq

et al. 2022

Front. Immunol.

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“…It has been known that NAEs, particularly NSE, exhibit adaptogenic, membrane-protective, antioxidant, immunomodulatory, anti-inflammatory, anti-allergic and anti-toxic effects [36]. Additionally, NSE modulated nitric oxide level (by affecting activi ty of constitutive and inducible NO synthases under different pathological conditions) [37] and reactive oxygen species level (by reducing the production of superoxide anion under LPS treatment) [38]. It worth to mentioning that effect of NSE is observed only under pathological conditions and does not show up under healthy normal conditions [39,40].…”

Section: Total Level Of Phospholipids and Level Of Individual Phosphomentioning

confidence: 99%

Preventive effect of N-stearoylethanolamine on memory disorders, blood and brain biochemical parameters in rats with experimental scopolamine-induced cognitive impairment

Horid’ko¹,

Kosiakova²,

Berdyshev³

2018

Ukr.Biochem.J

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The impairment of cognitive functions is the most studied medical and social problem nowadays. The aim of this study was to evaluate the protective effects of n-stearoylethanolamine (nSe) on memory state, blood and brain biochemical parameters in rats under scopolamine-induced cognitive impairment. The results of this study shown that nSe administration to rats per os (5 mg/kg, 5 days, during last 3 days nSe was administrated 20 min prior to scopolamine injection (1 mg/kg, once daily for 3 days, intraperitoneally)) prevented the development of memory impairment. In particular, NSe action was associated with the prevention of increase in acetylcholinesterase activity, changes in phospholipid, free and esterified cholesterol level in hippocampus and frontal cortex, and disruption in pro-/antioxidant balance in blood and studied brain sections. considering the above mentioned biological effects, nSe is a promising drug candidate for integrative therapy of cognitive impairment of different profiles.

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“…Previously, on the different models of pathological conditions we have shown that NSE decreased the production and content of in ammatory cytokines (IL-1β, TNF-α) [15]. Further studies reported that particular NSE effect was associated with the inhibition of nuclear NF-κB translocation in LPS-activated rat peritoneal macrophages [ 23 ]. It is known that LPS-induced in ammatory response could be inhibited by the activation of either PPAR-γ or LXRα [ 24 , 25 ].…”

Section: Discussionmentioning

confidence: 93%

The Involvement of PPARγ In Anti-Inflammatory Activity of N-Stearoylethanolamide

Kosiakova

Berdyshev

Dosenko

et al. 2021

Preprint

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N-stearoylethanolamide (NSE)– a cannabinoid-like compound with wide range of biological activity. Anti-inflammatory properties of NSE have been indicated on different animal models of pathological conditions. However, the molecular mechanisms of anti-inflammatory action of NSE remain unclear. In the current study, the involvement of PPARγ in the NF-kB -dependent anti-inflammatory action of NSE was evaluated using different methodological approach. First method - molecular modeling, evaluated the possibility of NSE to bind with PPAR. Then, in ex vivo experiment, using selective synthetic agonist of PPARα/γ LY-171883 and selective antagonist of PPARγ - GW9662, the role of PPARα /PPARγ in the NSE’s effect on nuclear NF-kB translocation was examined in LPS-activated rat peritoneal macrophages. Finally, the NSE action on the mRNA level of several PPARγ- dependent genes was studied in liver of insulin-resistant rats. The molecular docking results showed that NSE could bind to PPARγ and compete for the binding with antagonist GW9662 and agonist LY171883 in the active site of PPARγ. It also has been found that NSE prevented the LPS-induced NF-kB translocation into the nuclei of rat peritoneal macrophages during pre-treatment with NSE before LPS application. When NSE was added before GW9662 and LPS treatment, the level of NF-kB translocation and IL-1β content reduced to control cells’ levels. These data confirmed a competitive binding of NSE with GW9662 for the ligand-binding domen of PPARγ. In addition, NSE administration to insulin resistant rats changed the mRNA expression of several PPARγ target gens, including FATP1 and IL1-ra.

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Modulation of LPS-induced ROS production and NF-?B nuclear translocation by N-stearoylethanolamine in macrophages

Abstract: N-Stearoylethanolamine (NSe) is a minor lipid that belongs to the N-acylethanolamines family that K e y w o r d s: N-stearoylethanolamine, reactive oxygen species, translocation of NF-κB in macrophages inrat, lPS.

Cited by 4 publications

References 21 publications

Pasture intake protects against commercial diet-induced lipopolysaccharide production facilitated by gut microbiota through activating intestinal alkaline phosphatase enzyme in meat geese

Pasture intake protects against commercial diet-induced lipopolysaccharide production facilitated by gut microbiota through activating intestinal alkaline phosphatase enzyme in meat geese

Preventive effect of N-stearoylethanolamine on memory disorders, blood and brain biochemical parameters in rats with experimental scopolamine-induced cognitive impairment

The Involvement of PPARγ In Anti-Inflammatory Activity of N-Stearoylethanolamide

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