Modulation of mesenchymal stem cell chondrogenesis in a tunable hyaluronic acid hydrogel microenvironment

Toh, Wei Seong; Lim, Teck Chuan; Kurisawa, Motoichi; Spector, Myron

doi:10.1016/j.biomaterials.2012.01.065

Cited by 259 publications

(216 citation statements)

References 45 publications

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“…63 Although scaffolds composed of natural polymers are usually mechanically weak and have limited mechanical controllability, several methods have been developed to improve their mechanical performance, including incorporation of functional groups (eg, thiolate, acrylate, and tyramine), and several composition systems, eg, collagen-polyacrylamide, alginate-polyacrylamide, and agarose-poly(ethylene glycol) (PEG) have been generated. 64,65 For instance, human mesenchymal stem cells show neurogenic and osteogenic phenotypes when seeded onto collagen-polyacrylamide hydrogel scaffolds, with a stiffness similar to that in brain (0.1-1 kPa), muscle (8-17 kPa), and nascent bone (.34 kPa) tissue. 66 Therefore, nanofibrous scaffolds hold great potential as in vivo extracellular matrix mimics for tissue engineering applications.…”

Section: Nanofibrous Scaffolds In Tissue Engineering Extracellular Mamentioning

confidence: 99%

Techniques for fabrication and construction of three-dimensional scaffolds for tissue engineering

Lu¹,

Li²,

Chen³

2013

IJN

409

215

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Three-dimensional biomimetic scaffolds have widespread applications in biomedical tissue engineering because of their nanoscaled architecture, eg, nanofibers and nanopores, similar to the native extracellular matrix. In the conventional "top-down" approach, cells are seeded onto a biocompatible and biodegradable scaffold, in which cells are expected to populate in the scaffold and create their own extracellular matrix. The top-down approach based on these scaffolds has successfully engineered thin tissues, including skin, bladder, and cartilage in vitro. However, it is still a challenge to fabricate complex and functional tissues (eg, liver and kidney) due to the lack of vascularization systems and limited diffusion properties of these large biomimetic scaffolds. The emerging "bottom-up" method may hold great potential to address these challenges, and focuses on fabricating microscale tissue building blocks with a specific microarchitecture and assembling these units to engineer larger tissue constructs from the bottom up. In this review, state-of-the-art methods for fabrication of three-dimensional biomimetic scaffolds are presented, and their advantages and drawbacks are discussed. The bottom-up methods used to assemble microscale building blocks (eg, microscale hydrogels) for tissue engineering are also reviewed. Finally, perspectives on future development of the bottom-up approach for tissue engineering are addressed.

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Section: Nanofibrous Scaffolds In Tissue Engineering Extracellular Mamentioning

confidence: 99%

Techniques for fabrication and construction of three-dimensional scaffolds for tissue engineering

Lu¹,

Li²,

Chen³

2013

IJN

409

215

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“…Sato et al [14] set up a model of spontaneous osteoarthritis in the Hartley strain guinea pigs' knee joints, indicating that intra-articular transplantation of mesenchymal stem cells (MSCs) [15] used covalent cross-linking in vivo by adding peroxidase and hydrogen peroxide (H 2 O 2 ) to form an injectable and biodegradable hydrogel system made up of hyaluronic acid-tyramine (HA-Tyr) conjugate. The observations suggest that cellular density could be modulated by the changeable 3D microenvironment of the HA-Tyr hydrogels during chondrogenesis.…”

Section: Direct Transplantation Of Mesenchymal Stem Cells Into Knee Jmentioning

confidence: 99%

Liu¹,

Yu²

2017

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Osteoarthritis has been a common disease among elderly people, but there is not a solution clinically efficient for osteoarthritis. Therefore, tissue engineering has been developed in the field of cartilage regeneration. Tissue engineering has three basic elements: cell, scaffold and growth factor. An injectable scaffold, which can form a gel with original liquid form at the site of defect by injection, is an emerging scaffold for cartilage engineering. A scaffold can be composed of many materials such as natural materials, synthetic materials, composite materials, as well as hybrid materials. This paper reviews natural materials for cartilage regeneration.

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“…have shown favorable MSC attachment on gelatin-hydroxyphenylpropionic acid and HA-based hydrogels cross-linked using horseradish peroxidase (HRP) and H 2 O 2 , relative to culture plates. 210,211 Using the same cross-linking reaction, dextran has also been conjugated to heparin and applied for cartilage TE. 163 Use of horseradish peroxidases has not been limited to dextran.…”

Section: Self-assembling Peptidesmentioning

confidence: 99%

Injectable hydrogels

Overstreet

Dutta

Stabenfeldt

et al. 2012

J Polym Sci B Polym Phys

161

124

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Hydrogels are promising for a variety of medical applications due to their high water content and mechanical similarity to natural tissues. When made injectable, hydrogels can reduce the invasiveness of application, which in turn reduces surgical and recovery costs. Key schemes used to make hydrogels injectable include in situ formation due to physical and/or chemical cross-linking. Advances in polymer science have provided new injectable hydrogels for applications in drug delivery and tissue engineering. A number of these injectable hydrogel systems have reached the clinic and impact the health care of many patients. However, a significant remaining challenge is translating the ever-growing family of injectable hydrogels developed in laboratories around the world to the clinic. V C 2012 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 50: 2012

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Modulation of mesenchymal stem cell chondrogenesis in a tunable hyaluronic acid hydrogel microenvironment

Cited by 259 publications

References 45 publications

Techniques for fabrication and construction of three-dimensional scaffolds for tissue engineering

Techniques for fabrication and construction of three-dimensional scaffolds for tissue engineering

Injectable hydrogels

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Modulation of mesenchymal stem cell chondrogenesis in a tunable hyaluronic acid hydrogel microenvironment

Cited by 259 publications

References 45 publications

Techniques for fabrication and construction of three-dimensional scaffolds for tissue engineering

Techniques for fabrication and construction of three-dimensional scaffolds for tissue engineering

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Injectable hydrogels

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Product

Resources

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