Modulation of mesenchymal stem cells behaviors by chitosan/gelatin/pectin network films

Li, Junjie; Sun, Hong; Zhang, Rui; Li, Ruyue; Yin, Yuji; Wang, Hui; Liu, Yuxi; Yao, Fanglian; Yao, Kangde

doi:10.1002/jbm.b.31715

Cited by 24 publications

(20 citation statements)

References 47 publications

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“…For example, studies by Donzelli et al and George et al showed that collagen scaffolds can induce osteoblast differentiation of MSCs. Similarly, our previous work also found that chitosan/gelatin/pectin composites can improve the osteogenic differentiation capacity of BM‐derived MSCs. Nevertheless, the short degradation time of chitosan/gelatin/pectin composites prevents its applications in bone tissue regeneration.…”

Section: Introductionsupporting

confidence: 66%

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Iota‐carrageenan/chitosan/gelatin scaffold for the osteogenic differentiation of adipose‐derived MSCsin vitro

Yang

Qian

et al. 2014

J Biomed Mater Res

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In this study, we have developed ι-carrageenan/chitosan/gelatin (CCG) scaffold containing multiple functional groups (-NH2 , -OH, -COOH, and -SO3 H) to resemble the native extracellular matrix (ECM), using the ion-shielding technology and ultrasonic dispersion method. Fourier transform infrared spectroscopy (FTIR) of the CCG scaffolds suggests that the formation of CCG network involves electrostatic interactions between ι-carrageenan (ι-CA) and chitosan/gelatin, and the covalent cross-linking among amino groups of chitosan and/or gelatin. Scanning electron microscopic (SEM) observation reveals that the porous structure of scaffolds can be modulated by the ratio of ι-CA to chitosan/gelatin. The swelling ratio of the hydrogels increases as the ι-CA contents increase. Using differential scanning calorimetry, we found that the double helix structure of ι-CA is only stabilized at low contents of ι-CA in the CCG scaffolds (e.g., 5 wt %). The scaffolds containing 5% ι-CA showed the best protein adsorption capacity (4.46 ± 0.63 μg protein/mg scaffold) and elastic modulus (5.37 ± 1.03 MPa). In addition, the CCG scaffolds exhibit excellent support for adipose-derived mesenchymal stem cells (ADMSCs) attachment and proliferation, and they can improve the osteogenic differentiation and neovascularization capacities of ADMSCs. Overall, we conclude that the CCG may represent an ideal scaffold material for bone tissue engineering.

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Section: Introductionsupporting

confidence: 66%

“…The osteogenic differentiation of MSCs can be also characterized by the calcium deposition level and the expression of type I collagen . Von Kossa and Alizarin Red staining were carried out to evaluate the calcium deposition level after 28 days of cell growth.…”

Section: Resultsmentioning

confidence: 99%

Iota‐carrageenan/chitosan/gelatin scaffold for the osteogenic differentiation of adipose‐derived MSCsin vitro

Yang

Qian

et al. 2014

J Biomed Mater Res

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“…Pectin (PEC) is a linear anionic polysaccharide which is incorporated into skeletal structures of plants (e.g., citrus fruit) . PEC and its derivatives have already been studied as biomaterial in membrane, film coatings and scaffolds for skin substitutes to improve cellular interaction and hydrophilicity of structure .…”

Section: Introductionmentioning

confidence: 99%

Fabrication of functionalized citrus pectin/silk fibroin scaffolds for skin tissue engineering

et al. 2018

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In this study, novel porous three-dimensional (3D) scaffolds from silk fibroin (SF) and functionalized (amidated and oxidized) citrus pectin (PEC) were developed for skin tissue engineering applications. Crosslinking was achieved by Schiff's reaction in borax presence as crosslinking coordinating agent and CaCl addition. After freeze-drying and methanol treatment, plasma treatment (10 W, 3 min) was applied to remove surface skin layer formed on scaffolds. 3D matrices had high porosity (83%) and interconnectivity with pore size about 120 µm that providing suitable microenvironment for cells. Modifications on PEC chain and crosslinking of scaffolds were verified by fourier-transform infrared spectroscopy (FTIR) analysis and spectrophotometric assay. Scaffolds showed low weight loss (21.3% in 40 days) and high water uptake ability in phosphate-buffered saline (800% in 24 h). Mechanical properties of 3D matrices satisfied the stability of scaffolds under compressive stress and supported adhesion, proliferation and penetration of fibroblast cells. Our results suggested that modified PEC-SF scaffolds would be proposed for use in tissue engineered skin dermal substitutes. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2625-2635, 2018.

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“…[19][20][21][22] It is enriched in galacturonic acid and galacturonic acid methyl ester units. 24, 25 Li et al 26 showed that the introduction of pectin could improve the proliferative capacity and osteogenic differentiation of MSCs. 23 Moreover, due to its highly biocompatible and biodegradable nature, pectin serves as a novel biomaterial to modulate cell behaviour; pectin-containing matrices can improve the adhesion and proliferation of osteoblasts.…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of calcium phosphate/pectin scaffolds for bone tissue engineering

Zhao

Zhang

et al. 2016

RSC Adv.

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The calcium phosphate cement (CPC) scaffold has been used to repair bone defects, but its low compressive strength and poor osteogenesis greatly hinder its clinical application. Pectin is a natural, biocompatible, biodegradable, water-soluble polysaccharide that can improve the osteogenic activity of biomaterials. In this study, calcium phosphate/pectin cement (CPCP) scaffolds with different pectin concentrations (2%-6%) were prepared. The results showed that changes in the pectin concentration could modulate the morphology of the CPC scaffold. Moreover, CPCP scaffolds can significantly improve the compressive strength of the CPC. For example, the compressive strength reached 28.76 MPa when the concentration of pectin was 6%, which is similar to the mechanical strength of cancellous bone. In addition, the effects of the CPCP scaffolds on cell behaviour were investigated using a model of human adipose-derived stem cells (hADSCs). Our data showed that the introduction of pectin promoted the attachment and proliferation of hADSCs in vitro. Moreover, hADSCs seeded onto the CPCP scaffold showed higher osteogenetic efficacy than those seeded onto the CPC scaffold by modulating the expression of osteogenic genes. Based on these results, a pre-clinical study was performed in New Zealand white rabbits using the cavity defect model. Furthermore, new bone could be observed surrounding the CPCP scaffold within 8 weeks. These results indicate that the CPCP scaffold has potential applications in repairing bone defects.

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Modulation of mesenchymal stem cells behaviors by chitosan/gelatin/pectin network films

Cited by 24 publications

References 47 publications

Iota‐carrageenan/chitosan/gelatin scaffold for the osteogenic differentiation of adipose‐derived MSCsin vitro

Iota‐carrageenan/chitosan/gelatin scaffold for the osteogenic differentiation of adipose‐derived MSCsin vitro

Fabrication of functionalized citrus pectin/silk fibroin scaffolds for skin tissue engineering

Preparation and characterization of calcium phosphate/pectin scaffolds for bone tissue engineering

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