Fabrication of functionalized citrus pectin/silk fibroin scaffolds for skin tissue engineering

Türkkan, Sibel; Atila, Deniz; Akdağ, Akın; Tezcaner, Ayşen

doi:10.1002/jbm.b.34079

Cited by 52 publications

(23 citation statements)

References 34 publications

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“…Therefore, prepared hydrogels are able to provide not only sufficient room for MSCs migration but also a proper curvature for cell differentiation. The values of pore diameter were smaller than those reported by Turkkan et al . Higher concentration of hydrogel precursor solution (8 vs. 4%) and higher crosslinking extent can be taken account of possible reasons for decrease of the pore diameter.…”

Section: Resultscontrasting

confidence: 69%

“…The bands appeared at 3431, 2938, 1734, and 1622 cm −1 corresponded to respective OH vibration, COOCH 3 stretching vibration, either COOH stretching vibration or HCO vibration, and RCOR' stretching vibration . In oxidized polysaccharides, aldehyde related bands usually appears in the range of 1700–1750 cm ‐1 . Herein, aldehyde vibration band has overlapped with carboxyl vibration.…”

Section: Resultsmentioning

confidence: 93%

“…Moreover, the rheology of hydrogels has not been studied. Turkkan et al . have developed a porous skin scaffold from a combinatory composition of functionalized pectin and fibroin.…”

Section: Introductionmentioning

confidence: 99%

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High‐strength functionalized pectin/fibroin hydrogel with tunable properties: A structure–property relationship study

Khorshidi

Karkhaneh

Bonakdar

et al. 2019

J of Applied Polymer Sci

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Hydrogels possessing high mechanical strength and well-controlled properties are of utmost interest in bone tissue engineering. In the current study, a strong self-crosslinked hydrogel with variable properties has been developed from a combinatory composition of functionalized pectin and silk fibroin. Pectin chains underwent partial oxidation to be decorated with aldehyde functionalities. Later on, functionalized polysaccharide and protein were blended with different ratios, and corresponding variations in crystalline structure as well as in morphological, mechanical, and rheological properties have been monitored. Some theories have been used to better perceive the relation between blending ratio and hydrogel properties. Avrami equation based on time sweep rheological analysis elucidated that in sample with equivalent ratio of oxidized pectin and fibroin, the crystallization was highly delayed due to dominance of Schiff's base reaction. The Herschel-Bulkley model based on flow curve rheology test corroborated that aforesaid composition revealed the most noticeable shear thinning behavior and accordingly the strongest crosslinked network. Finally, rubber elastic theory based on dynamic mechanical analysis implied that said formulation possessed the highest crosslinking density due to higher availability of aldehyde to amine functionalities. Moreover, cell culture studies proved that P50 F50 sample prevailed over other compositions with respect to cell viability and function.

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Section: Resultscontrasting

confidence: 69%

Section: Resultsmentioning

confidence: 93%

See 1 more Smart Citation

High‐strength functionalized pectin/fibroin hydrogel with tunable properties: A structure–property relationship study

Khorshidi

Karkhaneh

Bonakdar

et al. 2019

J of Applied Polymer Sci

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“…These may be related to the clogging of needles in the case of 3D bioprinting and electrospinning (due to the rapid transition into β -sheet structure at the time of extrusion), inappropriate mechanical properties due to the length of degumming time [91], and the LiBr dissolution process [90] (which often leads to the degradation of protein chains). In order to circumvent this problem, silk-based composites have been developed for potential applications in different layers of skin such as 3D porous SF functionalized with citrus pectin [92], SF/sodium alginate freeze-dried scaffolds [93], electrospun nanofibers of SF with PLGA [94], SF only [38], collagen-SF [95], and 3D bioprinted SF with keratin [96] and gelatin [97].…”

Section: Biomaterials For Scaffold Fabricationmentioning

confidence: 99%

Functional Skin Grafts: Where Biomaterials Meet Stem Cells

Kaur

Midha

Giri

et al. 2019

Stem Cells International

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Skin tissue engineering has attained several clinical milestones making remarkable progress over the past decades. Skin is inhabited by a plethora of cells spatiotemporally arranged in a 3-dimensional (3D) matrix, creating a complex microenvironment of cell-matrix interactions. This complexity makes it difficult to mimic the native skin structure using conventional tissue engineering approaches. With the advent of newer fabrication strategies, the field is evolving rapidly. However, there is still a long way before an artificial skin substitute can fully mimic the functions and anatomical hierarchy of native human skin. The current focus of skin tissue engineers is primarily to develop a 3D construct that maintains the functionality of cultured cells in a guided manner over a period of time. While several natural and synthetic biopolymers have been translated, only partial clinical success is attained so far. Key challenges include the hierarchical complexity of skin anatomy; compositional mismatch in terms of material properties (stiffness, roughness, wettability) and degradation rate; biological complications like varied cell numbers, cell types, matrix gradients in each layer, varied immune responses, and varied methods of fabrication. In addition, with newer biomaterials being adopted for fabricating patient-specific skin substitutes, issues related to escalating processing costs, scalability, and stability of the constructs under in vivo conditions have raised some concerns. This review provides an overview of the field of skin regenerative medicine, existing clinical therapies, and limitations of the current techniques. We have further elaborated on the upcoming tissue engineering strategies that may serve as promising alternatives for generating functional skin substitutes, the pros and cons associated with each technique, and scope of their translational potential in the treatment of chronic skin ailments.

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“…Silk fibroin (SF) is produced by Bombyx mori silkworm and is fibrous proteins composed essentially of fibroin [23, 24]. Due to prominent biological properties, biocompatibility, minimal inflammatory reaction, biodegradability, and permeability silk-based nanofibers are at the center of attention for tissue engineering approaches [25–28]. The high strength of the nanofibrous scaffolds composed of silk is commonly applied for tissue engineering with the ability to maintain cell morphology, proliferation, and differentiation [29, 30].…”

Section: Introductionmentioning

confidence: 99%

A novel egg-shell membrane based hybrid nanofibrous scaffold for cutaneous tissue engineering

et al. 2019

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BackgroundThe main issue in cutaneous regeneration is to develop engineered scaffolds based on natural extracellular matrix to promote dynamics of skin progenitor cells and accelerate differentiation into mature keratinocytes.MethodsIn this study, nanofibrous scaffolds composed of a blend poly (ɛ-caprolactone) (PCL), silk fibroin (SF), soluble eggshell membrane (SESM), and Aloe vera (AV) gel were developed by electrospinning method and human basal cells were used to examine differentiation capacity toward keratinocyte-like cells. For this propose, cells were allocated to four distinct groups; control, PCL/SF, PCL/SF/SESM, and PCL/SF/SESM/AV. In all groups, cells were incubated with differentiation medium. Morphology, composition, hydrophilicity and mechanical features of PCL/SF, PCL/SF/SESM and PCL/SF/SESM/AV nanofibers were studied by scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), water contact angle and tensile tests. To examine the orientation of basal cells to mature keratinocytes, we performed immunofluorescence analysis by monitoring cytokeratin-19. The expression of genes such as involucrin, keratin-14 and -5 was monitored by real-time PCR assay.ResultsPCL/SF, PCL/SF/SESM, and PCL/SF/SESM/AV had suitable physic chemical indices and biological activities to be applied as biomimetic scaffolds for the restoration cutaneous tissue. Compared to control, we found an increased basal cell proliferation at 7 and 14 days after plating on scaffolds and reach maximum levels in group PCL/SF/SESM/AV on day 14 (p < 0.05). Electron microscopy showed cell flattening, morphological adaptation. An integrated cell-to-cell connection was generated after cell seeding on scaffolds in all groups. Immunofluorescence imaging showed the ability of basal cells to synthesize cytokeratin-19 in PCL/SF, PCL/SF/SESM, and positive control cells after exposure to differentiation medium. However, these values were less in PCL/SF/SESM/AV compared to other groups. Real-time PCR analysis showed the potency of all scaffolds to induce the transcription of involucrin, keratin-14 and -5, especially involucrin in PCL/SF/SESM/AV group compared to the negative control.ConclusionModulation of scaffolds with natural biopolymers could enable us to synthesize structures appropriate for cutaneous regeneration.

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Fabrication of functionalized citrus pectin/silk fibroin scaffolds for skin tissue engineering

Cited by 52 publications

References 34 publications

High‐strength functionalized pectin/fibroin hydrogel with tunable properties: A structure–property relationship study

High‐strength functionalized pectin/fibroin hydrogel with tunable properties: A structure–property relationship study

Functional Skin Grafts: Where Biomaterials Meet Stem Cells

A novel egg-shell membrane based hybrid nanofibrous scaffold for cutaneous tissue engineering

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