Modulation of MnSOD protein in response to different experimental stimulation in Hyphantria cunea

Kim, Y.I.; Kim, H.J.; Kwon, Young-In; Kang, Young Jin; Lee, I.H.; Jin, Byung Rae; Han, Yeon Soo; Cheon, Hyang-Mi; Ha, Nam Gyu; Seo, Sook Jae

doi:10.1016/j.cbpb.2010.08.003

Cited by 23 publications

(18 citation statements)

References 60 publications

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“…Previous studies have shown that temperature stress can cause MnSOD induction in several species: Chinese hamster lung fibroblast cell [36], Hydra vulgaris [37] and Hyphantria cunea [30]. An earlier study indicated that the activity of CuZnSOD in B. tabaci was noticeably enhanced under cold and heat stress [15].…”

Section: Discussionmentioning

confidence: 99%

“…Preliminary studies showed that the overexpression of MnSOD can extend the life span of adult Drosophila melanogaster [27] and reduce oxidative stress in cells [28,29]. Recently, it was found that MnSOD may be involved in immune responses to stimulators, such as heat, coldness, starvation and heavy metals [30,31]. However, until now, information about the function of MnSOD in B. tabaci was still lacking.…”

Section: Introductionmentioning

confidence: 99%

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Cloning, Expression and Characterization of Mitochondrial Manganese Superoxide Dismutase from the Whitefly, Bemisia tabaci

Xianlong

Wang

et al. 2013

IJMS

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A mitochondrial manganese superoxide dismutase from an invasive species of the whitefly Bemisia tabaci complex (Bt-mMnSOD) was cloned and analyzed. The full length cDNA of Bt-mMnSOD is 1210 bp with a 675 bp open reading frame, corresponding to 224 amino acids, which include 25 residues of the mitochondrial targeting sequence. Compared with various vertebrate and invertebrate animals, the MnSOD signature (DVWEHAYY) and four conserved amino acids for manganese binding (H54, H102, D186 and H190) were observed in Bt-mMnSOD. Recombinant Bt-mMnSOD was overexpressed in Escherichia coli, and the enzymatic activity of purified mMnSOD was assayed under various temperatures. Quantitative real-time PCR analysis with whiteflies of different development stages showed that the mRNA levels of Bt-mMnSOD were significantly higher in the 4th instar than in other stages. In addition, the in vivo activities of MnSOD in the whitefly were measured under various conditions, including exposure to low (4 °C) and high (40 °C) temperatures, transfer from a favorable to an unfavorable host plant (from cotton to tobacco) and treatment with pesticides. Our results indicate that the whitefly MnSOD plays an important role in cellular stress responses and anti-oxidative processes and that it might contribute to the successful worldwide distribution of the invasive whitefly.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cloning, Expression and Characterization of Mitochondrial Manganese Superoxide Dismutase from the Whitefly, Bemisia tabaci

Xianlong

Wang

et al. 2013

IJMS

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“…Different expression patterns of the SOD isoyzme have been detected in the midgut tissues of fourth-and fifthinstar gypsy moths, as well as under favorable and unfavorable conditions (Perić-Mataruga et al 2000). Different expressions of SOD and CAT isoforms have been shown in control and stressed larvae of Hyphantria cunea (Kim et al 2010) and Protaetia brevitarsis (Kim et al 2008). Also, the expression of several SOD isozymes and significantly increased intensity of two bands, as well as higher activity detected on the CAT zymogram, have been recorded in larvae of Chironomus ramosus exposed to a stressful condition (Datkhile et al 2009).…”

Section: Discussionmentioning

confidence: 93%

Effects of fluoranthene on the fitness-related traits and antioxidative defense in Lymantria dispar L.

Mrdaković

Ilijin

Vlahović

et al. 2015

Environ Sci Pollut Res

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This study aimed to examine the effects of ubiquitous polycyclic aromatic hydrocarbon fluoranthene, supplemented to an artificial diet, on the fitness-related traits and activity of midgut antioxidative enzymes-superoxide dismutase (SOD) and catalase (CAT), and expression of their isoforms in the fifth-instar gypsy moth Lymantria dispar L. Prolonged duration of development and reduced weight and relative growth rate were recorded in larvae reared on the diets supplemented with different concentrations of fluoranthene. SOD and CAT activities were significantly higher in the midguts of fluoranthene-treated larvae, compared to that of the control group. Different expression patterns were detected for SOD as well as for CAT isoforms, depending on the supplemented concentration of fluoranthene. Obtained results suggest that the activity of these enzymes in gypsy moth larvae may be used as biomarkers for assessing pollution, even at low concentrations of the pollutant.

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“…2006). Most recently, it was reported that SOD mRNA was predominantly detected in midgut, Malpighian tubule, epidermis and fat body, whereas it was almost undetectable in hemocytes in the fall webworm Hyphantria cunea (Kim et al . 2010).…”

Section: Resultsmentioning

confidence: 99%

Cloning and induction patterns of Cu/Zn superoxide dismutase in responses to immune elicitors and nucleopolyhedrovirus in the beet armyworm Spodoptera exigua

Noh

Park

et al. 2010

Entomological Research

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Superoxide dismutase (SOD) is a metalloenzyme that catalyzes the dismutation of the superoxide anion to oxygen and hydrogen peroxide, and has accordingly been known to play an important role in reducing oxidative stresses induced by various stresses and infection. In an attempt to investigate whether it is involved in antiviral immune responses in insects, the full‐length cDNA of SeCu/ZnSOD was obtained from the cDNA library of Spodoptera exigua. It is 1,008 bp long (excluding poly‐A tail) with the open reading frame of 459 bp encoding 152 amino acids. Sequence analysis with BLAST shows identities to various insects such as Bombyx mori (80%), Hyphantria cunea (80%), Plutella xylostella (77%), Aedes aegypti (73%), Apis mellifera (71%), Lasius niger (70%), Drosophila melanogaster (69%) and Gryllotalpa orientalis (67%). During development, S. exigua expressed SeCu/ZnSOD predominantly at embryonic stage, pupal stage and adult female stage, whereas it was slightly expressed in larvae and adult male. In addition, SeCu/ZnSOD was highly expressed in the fat body, hemocyte, silk gland and Malpighian tubule, although the levels of SeCu/ZnSOD transcripts were very low in the gut. Furthermore, SeCu/ZnSOD mRNA was slightly induced in response to either lipopolysaccharide or laminarin. Finally, SeCu/ZnSOD mRNA was induced in carcass in response to S. exigua nucleopolyhedrovirus (SeNPV) infection, whereas it was not strikingly induced in the gut. The precise roles of SeCu/ZnSOD in antiviral immune response remains to be further studied.

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Modulation of MnSOD protein in response to different experimental stimulation in Hyphantria cunea

Cited by 23 publications

References 60 publications

Cloning, Expression and Characterization of Mitochondrial Manganese Superoxide Dismutase from the Whitefly, Bemisia tabaci

Cloning, Expression and Characterization of Mitochondrial Manganese Superoxide Dismutase from the Whitefly, Bemisia tabaci

Effects of fluoranthene on the fitness-related traits and antioxidative defense in Lymantria dispar L.

Cloning and induction patterns of Cu/Zn superoxide dismutase in responses to immune elicitors and nucleopolyhedrovirus in the beet armyworm Spodoptera exigua

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