Modulation of organotin-induced apoptosis by the water pollutant methyl mercury in a human lymphoblastoid tumor cell line and a marine sponge

Batel, Renato; Bihari, Nevenka; Rinkevich, B.; Dapper, Jutta; Schäcke, Heike; Schröder, HC; Müller, Weg

doi:10.3354/meps093245

Cited by 56 publications

(20 citation statements)

References 6 publications

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“…44 ), inducible; it is selectively upregulated in response to non-toxic apoptotic stimuli. At higher TBT concentrations (410 mM) or more severe heat shock (148C above ambient temperature) the expressions of genes coding for BHP2_GC, HSP-70 and septin decline 26,45 (and unpublished).…”

Section: Transfection Of Sponge Bcl-2 Into Human Hek-293 Cells M Wienmentioning

confidence: 99%

“…The capacity of inducers to cause apoptosis depends on the respective species, tissues or cells (reviewed in 39 ). Therefore, in the present study the established stimuli for apoptosis in sponges, TBT 26 and heat shock 40 have been chosen. With respect to HEK-293 cells serum starvation and TBT, both triggering the cells to apoptosis dosedependently via the caspase pathway, 25,30 have been selected as inducers.…”

Section: Transfection Of Sponge Bcl-2 Into Human Hek-293 Cells M Wienmentioning

confidence: 99%

“…1 Exposure of sponge cells to the organic derivative of the heavy metal tin, tributyltin [TBT] and heat shock causes an increased expression of GCBHP2. TBT also causes an [Ca 2+ ] i overload, cytoskeletal damage and mitochondrial failure (see 25 ); and it was already earlier found that TBT induces apoptosis in sponges 26 and also in vertebrate cells. 25,27 Here, the molecular marker septin was used to establish the selective expression of GCBHP2.…”

Section: Introductionmentioning

confidence: 99%

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Sponge Bcl-2 homologous protein (BHP2-GC) confers distinct stress resistance to human HEK-293 cells

2001

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It is established that sponges, the phylogenetically oldest still extant phylum of Metazoa, possess key molecules of the apoptotic pathways, that is members from the Bcl-2 family and a pro-apoptotic molecule with death domains. Here we report on transfection studies of human cells with a sponge gene, GCBHP2. Sponge tissue was exposed to heat shock and tributyltin, which caused an upregulation of gene expression of GCBHP2. The cDNA GCBHP2 was introduced into human HEK-293 cells and mouse NIH-3T3 cells; the stable transfection was confirmed by the identification of the transcripts, by Western blotting as well as by immunofluorescence using antibodies raised against the recombinant polypeptide. HEK-293 cells, transfected with GCBHP2, showed high resistance to serum starvation and tributyltin treatment, compared to mock-transfected cells. In contrast to mock-transfected cells, GCBHP2-transfected cells activated caspase-3 to a lower extent. Thus, sponges contain gene(s) involved in apoptotic pathway(s) displaying their function also in human cells. Cell Death and Differentiation (2001) 8, 887 ± 898.

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Section: Transfection Of Sponge Bcl-2 Into Human Hek-293 Cells M Wienmentioning

confidence: 99%

Section: Transfection Of Sponge Bcl-2 Into Human Hek-293 Cells M Wienmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sponge Bcl-2 homologous protein (BHP2-GC) confers distinct stress resistance to human HEK-293 cells

2001

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“…in AIDS. A variety of extrinsic signals have been described to induce apoptosis (reviewed in: Vermes and Haanen, 1994); in our group we identified (i) physiological activators, neurotransmitters (Mu È ller et al, 1992), (ii) damage-related inducers, heat shock (Batel et al, 1993) and (iii) toxins, lead and prion protein as inducers of apoptosis. In AIDS patients excessive cell death has been described both for neurons and lymphocytes, a process which is assumed to be the major cause of this disease (Ameisen, 1992).…”

Section: Discussionmentioning

confidence: 99%

Flupirtine protects both neuronal cells and lymphocytes against induced apoptosis in vitro: Implications for treatment of AIDS patients

Müller

Dobmeyer

et al. 1997

Cell Death Differ

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In the present study we demonstrate that flupirtine, an already clinically used, centrally acting, non-opiate analgesic agent, protects rat cortical neurons against HIV-gp120 induced apoptotic cell death. The drug was active at concentrations between 1 and 10 mg/ml. Furthermore we show inhibition of in vitro induced apoptosis in human blood mononuclear cells, using flupirtine. Induced apoptosis in peripheral blood mononuclear cells from healthy individuals and HIV-1 infected patients was reduced to approximately 50% after in vitro preincubation with flupirtine at concentrations between 0.1 and 10 mg/ml. The anti-apoptotic effect of flupirtine was restricted to CD3 + lymphocytes and in particular to CD4 + cells. Flupirtine does not affect uninduced apoptosis in human lymphocytes in vitro. The selective potential of flupirtine to reduce apoptosis without influencing uninduced apoptosis may qualify this compound as a potential drug in the therapy of HIV-1 infected patients.

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“…Previously, it was described for the first time for sponges in particular and for Metazoa in general that xenobiotics, e.g. tributyltin and methyl mercury, cause apoptosis (Batel et al 1993). …”

Section: Proteinsmentioning

confidence: 99%

Analysis of the Sponge [Porifera] Gene Repertoire: Implications for the Evolution of the Metazoan Body Plan

Müller¹,

Müller²

2003

Sponges (Porifera)

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Abstract. Sponges [phylum Porifera] form the basis of the metazoan kingdom and represent the evolutionary earliest phylum still extant. Hence, as living fossils, they are the taxon closest related to the hypothetical ancestor of all Metazoa, the Urmetazoa. Until recently, it was still unclear whether sponges are provided with a defined body plan. Only after the cloning, expression and functional studies of characteristic metazoan genes, could it be demonstrated that these animals comprise the structural elements which allow the sponge cells to organize themselves according to a body plan. Adhesion molecules involved in cell-cell and cell-matrix interactions have been identified. Among the cell-cell adhesion molecules the aggregation factor (AF) is the prominent particle. It is composed of a core protein that is associated with the adhesion molecules, a 36 kDa as well as a 86 kDa polypeptide. A galee tin functions as a linker of the AF to the cell-membrane-associated receptor, the aggregation receptor (AR). The most important extracellular matrix molecules are collagen-and fibronectin-like molecules. These proteins interact with the cellmembrane receptors, the integrins. In addition, a neuronal receptor has been identified, which -together with the identified neuroactive molecules -indicate the existence of a primordial neuronal network already in Porifera. The primmorph system, aggregated cells that retain the capacity to proliferate and differentiate, has been used to demonstrate that a homeobox-containing gene, Iroquois, is expressed during canal formation in primmorphs. The formation of a body plan in sponges is supported by skeletal elements, the spicules, which are composed in Demospongiae as well as in Hexactinellida of amorphous, noncrystalline silica. In Demospongiae the spicule formation is under enzymic control of silicatein. Already at least one morphogen has been identified in sponges, myotrophin, which is likely to be involved in the axis formation. Taken together, these elements support the recent conclusions that sponges are not merely non organized cell aggregates, but already complex animals provided with a defined body plan.

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Modulation of organotin-induced apoptosis by the water pollutant methyl mercury in a human lymphoblastoid tumor cell line and a marine sponge

Cited by 56 publications

References 6 publications

Sponge Bcl-2 homologous protein (BHP2-GC) confers distinct stress resistance to human HEK-293 cells

Sponge Bcl-2 homologous protein (BHP2-GC) confers distinct stress resistance to human HEK-293 cells

Flupirtine protects both neuronal cells and lymphocytes against induced apoptosis in vitro: Implications for treatment of AIDS patients

Analysis of the Sponge [Porifera] Gene Repertoire: Implications for the Evolution of the Metazoan Body Plan

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