Modulation of osteogenic differentiation in hMSCs cells by submicron topographically-patterned ridges and grooves

Abstract

Recent studies have shown that nanoscale and submicron topographic cues modulate a menu of fundamental cell behaviors, and the use of topographic cues is an expanding area of study in tissue engineering. We used topographically-patterned substrates containing anisotropically-ordered ridges and grooves to investigate the effects of topographic cues on mesenchymal stem cell morphology, proliferation, and osteogenic differentiation. We found that human mesenchymal stem cells cultured on 1400 or 4000 nm pitches showed greater elongation and alignment relative to 400 nm pitch or planar control. Cells cultured on 400 nm pitch demonstrated significant increases in RUNX2 and BGLAP expression relative to cells cultured on 1400 or 4000 nm pitch or planar control. 400 nm pitch enhanced extracellular calcium deposition. Cells cultured in osteoinductive medium revealed combinatory effects of topography and chemical cues on 400 nm pitch as well as up-regulation of expression of ID1, a target of the BMP pathway. Our data demonstrate that a specific size scale of topographic cue promotes osteogenic differentiation with or without osteogenic agents. These data demonstrate that the integration of topographic cues may be useful for the fabrication of orthopedic implants.

Influence of surface microstructure and chemistry on osteoinduction and osteoclastogenesis by biphasic calcium phosphate discs

“…Alternatively, decades of research have shown that surface topography can directly stimulate bone cell differentiation and function on various material substrates, including polymers (Fu et al, 2010;Watari et al, 2012;Wilkinson et al, 2011;You et al, 2010), titanium (Brunette, 1988;Gittens et al, 2011;, ceramics (Webster, 2000;Zhang et al, 2014), and tissue (Gray et al, 1996). Topographical control of cell fate is a complex phenomenon that can occur through focal adhesion clustering and downstream focal adhesion kinase (FAK) signalling (McNamara et al, 2010).…”

Nanoengineered implant as a new platform for regenerative nanomedicine using 3D well-organized human cell spheroids

“…In particular, there has been increasing interest in developing smart scaffolds mimicking the extracellular matrix, in order to favor long-term cell grafting. 1 Structural design of biomimetic scaffolds has been improved at the micro-and nanoscale using designed nanostructures, from nanofibers, 2,3 to nanogrooves, 4,5 and using different nanomaterials such as carbon nanotubes 6,7 and graphene material. 8,9 Nanotechnologies like nanoparticles or nanoreservoirs have also been introduced for the bioactivation of the scaffolds through the controlled release of bioactive therapeutics.…”

Advanced Nanostructured Medical Device Combining Mesenchymal Cells and VEGF Nanoparticles for Enhanced Engineered Tissue Vascularization

“…To overcome the complications associated with these methodologies (site morbidity, limited availability and host tissue reactivity), cell-based tissue engineering strategies using sophisticated biomaterials have been developed and represent a promising part of the regenerative medicine field. In the recent years, a plethora of advanced biomaterials mimicking extracellular matrix were designed based on the micro-and nano-scale environment of tissues, using nanotechnologies as graphene, nanogrooves and carbon nanotubes [1][2][3][4][5][6][7][8]. More recently, and with the development of the third generation materials, there has been increasing interest in developing nanoparticles and nanoreservoirs containing active molecules able to bio-activate scaffolds [9][10][11][12][13][14].…”