Modulation of paraoxonase 1 and 3 expression after moderate exercise training in the rat

Romani, Rita; Medio, Gianna Evelina De; Tullio, Simona di; Lapalombella, Rosa; Pirisinu, Irene; Margonato, V.; Veicsteinas, Arsenio; Marini, Marina; Rosi, Gabriella

doi:10.1194/jlr.m800493-jlr200

Cited by 32 publications

(30 citation statements)

References 43 publications

(48 reference statements)

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“…Both mechanisms are likely to be associated in our experimental model. On this issue, animal [60,61] and human data [62,63] have provided conflicting results on the extent of exercisemediated detrimental effects on HDL-associated PON-1 activity, since modulation might be influenced by the intensity of exercise stimuli but also by the training status of individuals as well as by their genetic background, specifically in relation to PON1 polymorphisms [64,65]. This complex scenario could explain the high variability observed in HDLnormalized PON1 paraoxonase activities reported in Figure 7(A).…”

Section: Discussionmentioning

confidence: 99%

Effect of ubiquinol supplementation on biochemical and oxidative stress indexes after intense exercise in young athletes

et al. 2018

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Objectives: Physical exercise significantly impacts the biochemistry of the organism. Ubiquinone is a key component of the mitochondrial respiratory chain and ubiquinol, its reduced and active form, is an emerging molecule in sport nutrition. The aim of this study was to evaluate the effect of ubiquinol supplementation on biochemical and oxidative stress indexes after an intense bout of exercise. Methods: 21 male young athletes (26 + 5 years of age) were randomized in two groups according to a double blind cross-over study, either supplemented with ubiquinol (200 mg/day) or placebo for 1 month. Blood was withdrawn before and after a single bout of intense exercise (40 min run at 85% maxHR). Physical performance, hematochemical parameters, ubiquinone/ubiquinol plasma content, intracellular reactive oxygen species (ROS) level, mitochondrial membrane depolarization, paraoxonase activity and oxidative DNA damage were analyzed. Results: A single bout of intense exercise produced a significant increase in most hematochemical indexes, in particular CK and Mb while, on the contrary, normalized coenzyme Q 10 plasma content decreased significantly in all subjects. Ubiquinol supplementation prevented exercise-induced CoQ deprivation and decrease in paraoxonase activity. Moreover at a cellular level, in peripheral blood mononuclear cells, ubiquinol supplementation was associated with a significant decrease in cytosolic ROS while mitochondrial membrane potential and oxidative DNA damage remained unchanged. Discussion: Data highlights a very rapid dynamic of CoQ depletion following intense exercise underlying an increased demand by the organism. Ubiquinol supplementation minimized exerciseinduced depletion and enhanced plasma and cellular antioxidant levels but it was not able to improve physical performance indexes or markers of muscular damage.

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Section: Discussionmentioning

confidence: 99%

Effect of ubiquinol supplementation on biochemical and oxidative stress indexes after intense exercise in young athletes

et al. 2018

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“…Paraoxonases are a small family of antioxidant enzymes whose antiatherogenic activity is well known (26). They are synthesized in the liver and in serum are located in HDL (27).…”

Section: Discussionmentioning

confidence: 99%

“…Cakmak et al (28) showed that PON activity was greater in adolescent athletes, suggesting that regular exercise might provide a cardio-protective effect. On the other hand, Romani et al (26) reported that physical stress, such as acute exercise, by altering membrane composition, may impair PON release from liver membranes and can decrease the level of PON in serum. Moreover, Manresa et al (13) reported that there is no change of PON activity during exercise.…”

Section: Discussionmentioning

confidence: 99%

Effects of Silymarin Supplementation on Leptin, Adiponectin and Paraoxanase Levels and Body Composition During Exercise: A Randomized Double-Blind Placebo Controlled Clinical Trial

Shirali

Mashhadi

Ashtary‐Larky

et al. 2016

Jundishapur J Nat Pharm Prod

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Background: Silymarin has powerful antioxidant properties, but its effects on athletic performance are poorly understood. Objectives: The present study was undertaken to evaluate the effects of 4 weeks of endurance or strength exercise, with or without silymarin, on body composition, paraoxonase (PON), leptin and adiponectin levels in untrained men. Methods: A total of 45 untrained men were divided into 5 groups (n = 9): endurance training with placebo (ET + P), endurance training with 140 mg of silymarin/day (ET + S), strength training with placebo (ST + P), strength training with 140 mg of silymarin/day (ST + S) and placebo (C). Anthropometrical and VO2max measurements and ELISA assay for PON, leptin and adiponectin levels were performed at the beginning and after the 4-week of the study Results: There was a significant decrease in weight and body mass index in the ET + P and ET + S groups and increases in ST + P and ST + S (P < 0.05) groups. Body fat declined in all four trained groups (P < 0.001). Peak oxygen uptake (VO2max) improved in both ET + P and ET + S subjects. Paraoxonase (PON) was increased only in ET + S group (P < 0.05). Adiponectin was increased in all four groups (P < 0.05). Circulating leptin remained unchanged within all interventions. Conclusions:The present study has demonstrated that a combination of exercise and silymarin can improve body composition and adiponectin levels.

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“…Therefore, the relationships found in the present study may reflect developments in PON1 activities. Some studies showed that there were changes in PON1 activity or antioxidant capacity of HDLs in the absence of changes in BLLP levels and antioxidant capacity 56,57) . These study results support our proposition that exercise can change PON1 release and its activity by internal developments in organisms without any quantitative changes in other factors, such as physical composition, performance, and BLLP levels in welltrained athletes Post-T.…”

Section: Training Effects On Blood Tdpon1 Activities and Pon1p Involvmentioning

confidence: 99%

Effects of Anaerobic Training on Paraoxonase-1 Enzyme (PON1) Activities of High Density Lipoprotein Subgroups and Its Relationship with PON1-Q192R Phenotype

Turgay

Şişman

Aksu

2015

JAT

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Aim: Paraoxonase-1 (PON1) is an antiatherosclerotic enzyme located on high-density lipoprotein (HDL). The effects of anaerobic exercise on PON1 activity are unknown. Here we investigated the effects of anaerobic judo training on three different activities of same PON1 enzyme (TDPON1), including basal PON1, salt-stimulated PON1 (SPON1), and arylesterase (AE) activities, of serum, HDL, and HDL subgroups (HDLs; HDL and its subgroups) and its relationship with PON1-Q192R phenotype (PON1P). Methods: Our study included 18 Turkish national female judoists (mean age: 17.9 0.8 years). Before and after 5 months of anaerobic training, critical speed (CS), TDPON1 activities, cholesterol levels in the serum and supernatants of HDLs obtained by polyethylene glycol, and other major blood lipids and lipoproteins (BLLPs) including triglycerides were determined using blood samples taken after overnight fasting. PON1P groups (PGs) were categorized as QQ (QG; persons with low activity) and R carriers (QR RR) (RG; persons with high activity) according to SPON1/AE activity ratios. The results were considered statistically significant at P ≤ 0.05. Results: Anaerobic training resulted in significantly increased the cholesterol levels of HDLs (except HDL2-C) in all subjects, but not HDLs-C in PGs. Anaerobic training resulted in significant increases in most TDPON1 activities of serum and HDLs in all subjects and (except AE) in PGs, whereas SPON1 and HDL2 AE activities increased only in the RG, which was related to PON1P. However, PON1P was not related to other measured markers, including basal BLLP profiles. Conclusions: Anaerobic training improved most TDPON1 activities of serum and HDLs and HDLs -C levels (except HDL2-C) in all subjects, but not HDLs-C in PGs. The beneficial effects of anaerobic training on SPON1 and HDL2 AE activities were depend on PON1P. The lack of response of HDL2-C to anaerobic exercise will require further research. J Atheroscler Thromb, 2015; 22: 313-326.

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Modulation of paraoxonase 1 and 3 expression after moderate exercise training in the rat

Cited by 32 publications

References 43 publications

Effect of ubiquinol supplementation on biochemical and oxidative stress indexes after intense exercise in young athletes

Effect of ubiquinol supplementation on biochemical and oxidative stress indexes after intense exercise in young athletes

Effects of Silymarin Supplementation on Leptin, Adiponectin and Paraoxanase Levels and Body Composition During Exercise: A Randomized Double-Blind Placebo Controlled Clinical Trial

Effects of Anaerobic Training on Paraoxonase-1 Enzyme (PON1) Activities of High Density Lipoprotein Subgroups and Its Relationship with PON1-Q192R Phenotype

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