Modulation of posterior intestinal mucosal proteome in rainbow trout (Oncorhynchus mykiss) after Yersinia ruckeri infection

Abstract

Yersinia ruckeri is the causative agent of enteric redmouth disease in salmonids. In fish, the intestine represents an important site of nutrient uptake, host–pathogen interactions, and defense. The posterior intestine can be inflamed, reddened, and filled with an opaque, yellowish fluid during Y. ruckeri infection. Herein, we report an investigation on the proteome alteration in the posterior intestinal mucosa of rainbow trout ( Oncorhynchus mykiss ) after exposure to Y. ruckeri . The intestinal mucosal proteins were identified and quantified by a shotgun proteomic approach by applying data-independent quantification with sequential windowed acquisition of all theoretical mass spectra (SWATH). A total of 437 proteins were found to be differentially up- or downregulated in the posterior intestine. Gene ontology of upregulated proteins pointed to their involvement into exopeptidase, endopeptidase, and hydrolase activities, while the downregulated proteins were involved in lipid metabolism, actin binding, and translation processes. Additionally, upregulated proteins were predicted to be involved in lysosome, oxidative phosphorylation, and metabolic pathways, while downregulated proteins were implicated in focal adhesion, regulation of actin cytoskeleton, protein digestion and absorption pathways. This study showed that Y. ruckeri infection can alter protein abundance involved in serine-type carboxypeptidase, cysteine and aspartic-type endopeptidases, metallopeptidases, antioxidant defense, calcium ion binding, glycolytic and carbohydrate metabolic processes in the proteome of the intestinal mucosa of rainbow trout. Electronic supplementary material The online version of this article (10.1186/s13567-019-0673-8) contains supplementary material, which is available to authorized users.

Impact of high dietary plant protein with or without marine ingredients in gut mucosa proteome of gilthead seabream (Sparus aurata, L.)

“…The study of the gut mucosa using omics could help for a better understanding of the relation between nutritional changes and fish performance, as well as of its role in intestinal health, contributing to the feasibility of incorporating high dietary levels of plant proteins in aquafeeds for carnivorous species. In this regard, proteomic studies have been carried out in numerous species [23], such as Atlantic salmon (Salmo salar) [24,25], rainbow trout (Oncorhynchus mykiss) [26][27][28][29], Atlantic cod [30], common carp [31,32], zebrafish (Danio rerio) [33], Nile tilapia (Oreochromis niloticus) [34,35] and also in gilthead seabream [36][37][38]. However, most of these works focused on the response of the liver metabolism to dietary changes and few authors have studied the gut response to changes in dietary composition or feeding pattern [26,27,39,40].…”

Physiological Benefits of Dietary Lysophospholipid Supplementation in a Marine Fish Model: Deep Analyses of Modes of Action

“…Proteomics is widely used to understand fish physiology, as this technique can show differential expression of identified proteins at various stages of fish development and under different conditions of feeding, stress, or disease, thereby providing a holistic overview of several functions in fish metabolism [32]. Here, using shotgun proteomics, we identified a high number of proteins (4850 and 4984 for the intestine and liver, respectively), like other studies using shotgun approaches [33][34][35] which were greater than those of other studies using 2D proteomics in fish tissues [19,36]. Next, we used GO enrichment analysis for the DEPs, similar to recent proteomic evaluations in fish [35,37], mainly searching for the biological processes and Reactome pathways affected by dietary LPLs.…”

Fish Pathology Research and Diagnosis in Aquaculture of Farmed Fish; a Proteomics Perspective

“…Enteric redmouth disease in salmonids resulted in several differentially expressed proteins in head kidney and liver samples of rainbow trout like antioxidants, lysozyme, metalloproteinase, cytoskeleton and c-type lectin receptor proteins [ 95 ]. Up-regulated proteins involved in peptidase and hydrolase activity, lysosome and metabolic pathways were identified in intestinal mucosal samples [ 196 ]. Detected on the first defence barrier of fish, the skin mucus showed differentially expressed proteins of the immune system of Atlantic cod with vibriosis [ 83 ].…”