Modulation of radiation induced lipid peroxidation by phospholipase A2 and calmodulin antagonists: Relevance to detoxification

Varshney, Rajeev K.; Kale, R.K.

doi:10.1016/0969-806x(94)00077-w

Cited by 8 publications

(6 citation statements)

References 30 publications

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“…Repair is completed by reacylation with a fattyacyl-coenzyme A [21]. Since CPZ is known to mobilise Ca 2+ ions, it would help in the removal of peroxidised fatty acids from membranes by phospholipase A 2 and the subsequent disposal of the peroxides [22][23][24] through the mobilization of Ca 2+ ions, providing protection against lipid peroxidation. In chain reactions, there are many potentially competing reactions for initiation, propagation, and termination.…”

Section: Resultsmentioning

confidence: 99%

Effect of high linear energy transfer radiation on biological membranes

Choudhary

Srivastava

Sarma

et al. 1998

Radiation and Environmental Biophysics

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Cellular membranes are vital elements, and their integrity is extremely essential for the viability of the cells. We studied the effects of high linear energy transfer (LET) radiation on the membranes. Rabbit erythrocytes (1 x 10(7) cells/ml) and microsomes (0.6 mg protein/ml) prepared from liver of rats were irradiated with 7Li ions of energy 6.42 MeV/u and 16O ions of energy 4.25 MeV/u having maximum LET values of 354 keV/microm and 1130 keV/microm, respectively. 7Li- and 16O-induced microsomal lipid peroxidation was found to increase with fluence. The 16O ions were more effective than 7Li ions, which could be due to the denser energy distribution in the track and the yield of free radicals. These findings suggested that the biological membranes could be peroxidized on exposure to high-LET radiation. Inhibition of the lipid peroxidation was observed in the presence of a membrane-active drug, chlorpromazine (CPZ), which could be due to scavenging of free radicals (mainly HO* and ROO*), electron donation, and hydrogen transfer reactions. The 7Li and 16O ions also induced hemolysis in erythrocytes. The extent of hemolysis was found to be a function of time and fluence, and showed a characteristic sigmoidal pattern. The 16O ions were more effective in the lower fluence range than 7Li ions. These results were compared with lipid peroxidation and hemolysis induced by gamma-radiation.

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Section: Resultsmentioning

confidence: 99%

Effect of high linear energy transfer radiation on biological membranes

Choudhary

Srivastava

Sarma

et al. 1998

Radiation and Environmental Biophysics

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“…This was assayed by measuring the TBA (thiobarbituric acid), reactive products present in the treated and untreated plant samples using the procedure of Vashney and Kale [44] and expressed as micromolar of malondialdehyde (MDA)/g tissue. Lipid peroxidation was measured in terms of malondialdehyde (MDA) content.…”

Section: Determination Of Product Of Lipid Peroxidation (Mda)mentioning

confidence: 99%

“…On the other hand, the lowered MDA content indicates toxicity/poisoning in the test plant. MDA is the end product of peroxidation of membrane lipids and it accumulates when the plants are subjected to oxidative stress [44]. Generally, free radical generation and membrane damage would be low in tolerant plants and thereby formation of lower levels of MDA content [39].…”

Section: Biochemical Analysismentioning

confidence: 99%

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Response of Bambara groundnut (Vigna subterranean L.) and Maize (Zea mays L.) to heavy metal stress

Oladele

Adewumi

Yahaya

et al. 2019

Beni-Suef Univ J Basic Appl Sci

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Background: Plants are usually the target of environmental pollution. This study, therefore, investigates the effects of Zinc (Zn) and lead Pb on Bambara nut (Vigna subterranean) and Maize (Zea mays) at different concentrations, as well as the possible ameliorating effect of the chelant; ethylene diamine acetate (EDTA) and farmyard manure, on the enzymatic activities, the chlorophyll, total protein, and carbohydrate contents. Results: Findings revealed that Pb and Zn increased the superoxide dismutase (SOD), peroxidase, glutathione synthetase (GSH), malondialdehyde (MDA), and catalase levels with increased concentrations from 100 mg/kg to 200 mg/kg significantly (p < 0.05) compared to the control Maize plants. While in Bambara nut, the superoxide dismutase (SOD), peroxidase, glutathione synthetase (GSH), and catalase levels decreased with increased concentrations from 100 mg/kg to 200 mg/kg compared to control except for the malondialdehyde (MDA) which was increased. For Bambara groundnut, with increased Pb concentrations, the chlorophyll content reduced from 2.94 to 2.00 mg/g. However, there was an increase (up to 4.918 mg/g) in the chlorophyll content with increased zinc nitrate concentrations augmented with EDTA at the highest concentration. Maize plants treated with Pb augmented with farmyard manure showed an increase in chlorophyll content with increased concentrations while those assisted with EDTA still experienced a decrease as metal concentrations increased. Bambara groundnut plant had a mean carbohydrate (%) of 14.79 (control), 17.60 (100 mg/kg of Pb concentration) and 11.20 (200 mg/kg of Pb concentration), indicating a decrease in carbohydrate content with increased Pb concentrations. The same trend was observed for the different Zn and Pb concentrations on the mean total proteins and carbohydrates of both test plants. Generally, Pb and Zn induced oxidative stress in treated plants. Conclusions: Elevated activity of anti-oxidative enzymes can assist as important components of antioxidative defense mechanism against oxidative damage. The results of this study could be beneficial in the understanding of the role of the defense system as well as the detoxification mechanism of Vigna subterranean and Zea mays in efficient tolerance and response to Pb and Zn. This signifies that these plants can act as bioindicators in environmental quality assessment.

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“…Natural antioxidants have been considered to be promising protective agents against free radical mediated damage induced during cancer therapy. A number of effective compounds such as hoechst [ 14 ], cysteine [ 15 ], caffeine [ 16 ], calcium channel blockers [ 17 ], flavonoids [ 18 ], and 2-deoxy-D-glucose [ 19 ] have been identified. Natural antioxidants have also demonstrated effective protection against ROS mediated cellular damage during cancer chemotherapy and radiotherapy [ 20 – 22 ].…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of a Polyherbal Aqueous Extract Comprised ofNigella sativa(Seeds),Hemidesmus indicus(Roots), andSmilax glabra(Rhizome) on Bleomycin Induced Cytogenetic Damage in Human Lymphocytes

Galhena

Samarakoon

Thabrew

et al. 2017

BioMed Research International

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This study was carried out to determine the chemoprotective potential of a polyherbal aqueous decoction comprised of Nigella sativa (seeds), Hemidesmus indicus (roots), and Smilax glabra (rhizome) against bleomycin induced cytogenetic damage in human lymphocytes. Isolated peripheral blood lymphocytes (PBLs) were exposed to bleomycin at a dose of 40 µg/mL for 2 hrs in the presence or absence of different doses of the decoction (100, 300, and 600 µg/mL). Modulatory effect of the decoction on bleomycin induced cytogenetic damage was evaluated by (a) degree of chromosomal aberrations (CA), (b) formation of micronuclei (MN), and (c) induction of γH2AX foci in lymphocytes exposed to bleomycin. Lymphocytes pretreated with the decoction showed that a significant reduction (p < 0.05) in bleomycin induced (a) stable and unstable chromosome aberrations (CA), (b) MN formation, and (c) formation of γH2AX foci, when compared to lymphocytes treated only with bleomycin. The decoction by itself did not induce any significant cytogenetic damage in PBLs. Overall results of the present study confirm that the decoction can attenuate the cytogenetic damage mediated by bleomycin in human PBLs.

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Modulation of radiation induced lipid peroxidation by phospholipase A2 and calmodulin antagonists: Relevance to detoxification

Cited by 8 publications

References 30 publications

Effect of high linear energy transfer radiation on biological membranes

Effect of high linear energy transfer radiation on biological membranes

Response of Bambara groundnut (Vigna subterranean L.) and Maize (Zea mays L.) to heavy metal stress

Protective Effect of a Polyherbal Aqueous Extract Comprised ofNigella sativa(Seeds),Hemidesmus indicus(Roots), andSmilax glabra(Rhizome) on Bleomycin Induced Cytogenetic Damage in Human Lymphocytes

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