Modulation of ROS production in photodynamic therapy using a pH controlled photoinduced electron transfer (PET) based sensitiser

Atchison, Jordan; Kamila, Sukanta; McEwan, Conor; Nesbitt, Heather; Davis, James A.; Fowley, Colin; Callan, Bridgeen; McHale, Anthony P.; Callan, John F.

doi:10.1039/c5cc07022h

Cited by 23 publications

(13 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These substances are generally considered as the molecules involved in cellular death pathways . It has been established that intracellular ROS production induced by PDT causes cellular damage by attacking DNA, proteins and lipids , and mitochondrial ROS is considered as a molecule spreading via GJ channels as an oxidative stress signal . Therefore, intracellular ROS may play a role in Cx26‐composed GJIC‐dependent component of PDT phototoxicity.…”

Section: Discussionmentioning

confidence: 99%

Enhanced phototoxicity of photodynamic treatment by Cx26‐composed GJIC via ROS‐, calcium‐ and lipid peroxide‐mediated pathways

Lin

Bai

et al. 2017

Journal of Biophotonics

View full text Add to dashboard Cite

In spite of the promising initial treatment responses presented by photodynamic therapy (PDT), 5-year recurrence rates remain high level. Therefore, improvement in the efficacy of PDT is needed. There are reports showing that connexin(Cx) 26-composed gap junctional intercellular communication (GJIC) enhances the intercellular propagation of "death signal", thereby increasing chemotherapeutic cytotoxicity. However, it is unclear whether Cx26-formed GJIC has an effect on PDT phototoxicity. The results in the present study showed that Cx26-composed GJ formation at high density enhances the phototoxicity of Photofrin-PDT. When the Cx26 is not expressed or Cx26 channels are blocked, the phototoxicity in high-density cultures substantially reduces, indicating that the enhanced PDT phototoxicity at high density is mediated by Cx26-composed GJIC. The GJIC-mediated increase in PDT phototoxicity was associated with ROS, calcium and lipid peroxide-mediated stress signaling pathways. The work presents the ability of Cx26-composed GJIC to enhance the sensitivity of malignant cells to PDT, and indicates that maintenance or increase of Cx26-formed GJIC may be a profitable strategy towards the enhancement of PDT therapeutic efficiency.Picture: The survival response of Photofrin-PDT in Doxtreated (Cx26 expressing, GJ-formed) and Dox-untreated cells (Cx26 non-expressing, GJ-unformed) at high-cell density condition.

show abstract

Section: Discussionmentioning

confidence: 99%

Enhanced phototoxicity of photodynamic treatment by Cx26‐composed GJIC via ROS‐, calcium‐ and lipid peroxide‐mediated pathways

Lin

Bai

et al. 2017

Journal of Biophotonics

View full text Add to dashboard Cite

show abstract

“…This process of photosensitisation hugely relies upon the presence of molecular oxygen and forms the basis for a successful photodynamic therapy (PDT) [7]. After absorbing energy from the light source, the PS interacts with molecular oxygen via energy transfer process or electron transfer process and generates reactive oxygen species (ROS), which oxidise cellular and subcellular organelles to induce either apoptosis or necrosis leading to cell death [8,9]. Since cytotoxic ROS occurs only after irradiation of the PS, tumour tissues can be targeted with high precision.…”

Section: Introductionmentioning

confidence: 99%

Photodynamic Therapy of Ovarian Carcinoma Cells with Curcumin-Loaded Biodegradable Polymeric Nanoparticles

et al. 2019

View full text Add to dashboard Cite

Accumulation of photosensitisers in photodynamic therapy in healthy tissues is often the cause of unwanted side effects. Using nanoparticles, improved bioavailability and site-specific drug uptake can be achieved. In this study, curcumin, a natural product with anticancer properties, albeit with poor aqueous solubility, was encapsulated in biodegradable polymeric poly(lactic-co-glycolic acid) (PLGA) nanoparticles (CUR-NP). Dynamic light scattering, laser Doppler anemometry and atomic force microscopy were used to characterise the formulations. Using haemolysis, serum stability and activated partial thromboplastin time tests, the biocompatibility of CUR-NP was assessed. Particle uptake and accumulation were determined by confocal laser scanning microscopy. Therapeutic efficacy of the formulation was tested in SK-OV-3 human ovarian adenocarcinoma cells post low level LED irradiation by determining the generation of reactive oxygen species and cytotoxicity. Pharmacologic inhibitors of cellular uptake pathways were used to identify the particle uptake mechanism. CUR-NP exhibited better physicochemical properties such as stability in the presence of light and improved serum stability compared to free curcumin. In addition, the novel nanoformulation facilitated the use of higher amounts of curcumin and showed strong apoptotic effects on tumour cells.

show abstract

“…Excited triplet states are formed from excited singlet states via a process known as intersystem crossing (ISC). 1,10 As fluorescence occurs as a result of radiative deactivation from a fluorophores excited singlet state, the fluorescence and singlet oxygen quantum yields are invariably linked, with good fluorophores typically being poor sensitisers and vice versa. 11,12 Addition of heavy atoms to the structural skeleton of conjugated aromatics is known to increase the ISC process and improve singlet oxygen generation.…”

mentioning

confidence: 99%

Iodinated cyanine dyes: a new class of sensitisers for use in NIR activated photodynamic therapy (PDT)

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

Modulation of ROS production in photodynamic therapy using a pH controlled photoinduced electron transfer (PET) based sensitiser

Abstract: A new sensitiser (4) for use in photodynamic therapy (PDT) has been developed to enable control of ROS production as a function of pH. This pH dependent PDT behaviour was tested in HeLa cells and in SCID mice bearing human xenograft pancreatic cancer (BxPC-3) tumours.

Cited by 23 publications

References 17 publications

Enhanced phototoxicity of photodynamic treatment by Cx26‐composed GJIC via ROS‐, calcium‐ and lipid peroxide‐mediated pathways

Enhanced phototoxicity of photodynamic treatment by Cx26‐composed GJIC via ROS‐, calcium‐ and lipid peroxide‐mediated pathways

Photodynamic Therapy of Ovarian Carcinoma Cells with Curcumin-Loaded Biodegradable Polymeric Nanoparticles

Iodinated cyanine dyes: a new class of sensitisers for use in NIR activated photodynamic therapy (PDT)

Contact Info

Product

Resources

About