Modulation of TGF‐β1 production from human keratinocytes by UVB

Lee, Hyung‐Suk T.; Kooshesh, Fatemeh; Saunder, Daniel N; Kondo, Seiji

doi:10.1111/j.1600-0625.1997.tb00155.x

Cited by 63 publications

(38 citation statements)

References 34 publications

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“…Cell growth and proliferation and cell death were the most influenced biological pathways. The nodal molecule TGF-beta 1 is already known to be regulated by UVB irradiation and it has functions in skin inflammation and carcinogenesis [42], [43].…”

Section: Resultsmentioning

confidence: 99%

UVA and UVB Irradiation Differentially Regulate microRNA Expression in Human Primary Keratinocytes

et al. 2013

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MicroRNA (miRNA)-mediated regulation of the cellular transcriptome is an important epigenetic mechanism for fine-tuning regulatory pathways. These include processes related to skin cancer development, progression and metastasis. However, little is known about the role of microRNA as an intermediary in the carcinogenic processes following exposure to UV-radiation. We now show that UV irradiation of human primary keratinocytes modulates the expression of several cellular miRNAs. A common set of miRNAs was influenced by exposure to both UVA and UVB. However, each wavelength band also activated a distinct subset of miRNAs. Common sets of UVA- and UVB-regulated miRNAs harbor the regulatory elements GLYCA-nTRE, GATA-1-undefined-site-13 or Hox-2.3-undefined-site-2 in their promoters. In silico analysis indicates that the differentially expressed miRNAs responding to UV have potential functions in the cellular pathways of cell growth and proliferation. Interestingly, the expression of miR-23b, which is a differentiation marker of human keratinocytes, is remarkably up-regulated after UVA irradiation. Studying the interaction between miR-23b and its putative skin-relevant targets using a Luciferase reporter assay revealed that RRAS2 (related RAS viral oncogene homolog 2), which is strongly expressed in highly aggressive malignant skin cancer, to be a direct target of miR-23b. This study demonstrates for the first time a differential miRNA response to UVA and UVB in human primary keratinocytes. This suggests that selective regulation of signaling pathways occurs in response to different UV energies. This may shed new light on miRNA-regulated carcinogenic processes involved in UV-induced skin carcinogenesis.

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Section: Resultsmentioning

confidence: 99%

UVA and UVB Irradiation Differentially Regulate microRNA Expression in Human Primary Keratinocytes

et al. 2013

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“…Reepithelialization and angiogenesis are the most essential processes in the proliferative phase. During the process of wound healing, keratinocytes, fibroblasts, thrombocytes, and macrophages secrete TGF-β1 [41–44]. This protein exhibits a wide range of effects, influencing all types of cells involved in a wound healing process.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory and burn injury wound healing properties of the shell of Haliotis diversicolor

Zhicheng

et al. 2016

BMC Complement Altern Med

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BackgroundThe shell of Haliotis diversicolor, or shijueming (SJM), is a type of traditional Chinese medicine. The SJM has appeared in historical records as early as the third and fourth centuries. Historical records have revealed that SJM had mainly been used to treat eye diseases. After the Qing Dynasty (1757), records had emerged, detailing the use of SJM for treating skin injuries, particularly for treating poorly managed ulcers or traumatic wounds. Furthermore, in our anti-inflammation-screening system, SJM significantly inhibited the expression of pro-inflammatory proteins. Previous studies have yet to adopt an animal model to verify the phenomenon and described in the historical records regarding the efficacy of SJM in promoting wound healing. Besides, the mechanism of wound healing effect of SJM is also not clear.MethodsThis study applied in vitro and in vivo models, tissue section analysis, and western blotting to evaluate the effect of SJM on wound healing. The RAW 264.7 cells were used in anti-inflammatory activity assay and phagocytic assay. Male Wistar rats were used to evaluate the effect of SJM on burn injury healing. A copper block (2 × 2 cm, 150 g) preheated to 165 °C in a dry bath was used to contact the skin area for 10 s, thus creating a full-thickness burn injury. The results were analyzed by hematoxylin and eosin staining, picrosirius red staining and Western blotting.ResultsThe results revealed that in the in vitro model, the presence of SJM decreased the inducible nitric oxide synthase (iNOS) expression and enhanced the functions of macrophages. The results of the rat burn injury model revealed that SJM decreased neutrophil infiltration, promoted wound healing, thus increasing the collagen I content and promoting the expression of transforming growth factor-beta 1 (TGF-β1) protein. We speculate that the effect and mechanism of SJM on promoting wound healing is related to macrophage activation. In the inflammation phase, SJM alleviates inflammation by inhibiting iNOS expression and removing neutrophils through phagocytosis. Furthermore, SJM induces the secretion of TGF-β1, converting collagen during the tissue remodeling phase.ConclusionsAccording to our review of relevant literature, this is the first study that applied an evidence-based method to verify that SJM alleviates inflammation, enhances phagocytosis, and triggers wound healing after burn injury. The study findings reveal that SJM provides a promising therapeutic option for treating burn injury.

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“…Even at sub-erythemal doses, UVB reduces LC density, migration and maturation in the epidermis and regional lymphoid tissue [10][11][12][13][14]. It has been suggested that this immunosuppressive effect is in large part due to the effects of UVB in modulating cytokine gene expression in keratinocytes (KC) [15][16][17][18][19][20][21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Microarray analysis of UVB-regulated genes in keratinocytes: downregulation of angiogenesis inhibitor thrombospondin-1

Howell

Wang

Freed

et al. 2004

Journal of Dermatological Science

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Modulation of TGF‐β1 production from human keratinocytes by UVB

Cited by 63 publications

References 34 publications

UVA and UVB Irradiation Differentially Regulate microRNA Expression in Human Primary Keratinocytes

UVA and UVB Irradiation Differentially Regulate microRNA Expression in Human Primary Keratinocytes

Anti-inflammatory and burn injury wound healing properties of the shell of Haliotis diversicolor

Microarray analysis of UVB-regulated genes in keratinocytes: downregulation of angiogenesis inhibitor thrombospondin-1

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