Modulation of the constitutive or cytokine-induced bronchial epithelial cell functions in vitro by fluticasone propionate

Sabatini, Federica; Silvestri, Michela; Sale, Rosa; Serpero, Laura D.; Raynal, Maria E.; Blasi, P. Di; Rossi, Giovanni A.

doi:10.1016/s0165-2478(03)00142-1

Cited by 6 publications

(5 citation statements)

References 34 publications

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“…The local production and release of at least some of these factors and/or their activity on circulating polymorphonuclear leukocytes can be effectively inhibited by glucocorticosteroids, thus explaining the results here reported, i.e. the significant reduction of the nasal neutrophilic inflammation after treatment with budesonide inhalation suspension [33,34].…”

Section: Discussionsupporting

confidence: 71%

Activity of budesonide on nasal neutrophilic inflammation and obstruction in children with recurrent upper airway infections

Bellodi¹,

Tosca²,

Pulvirenti³

et al. 2006

International Journal of Pediatric Otorhinolaryngology

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Section: Discussionsupporting

confidence: 71%

Activity of budesonide on nasal neutrophilic inflammation and obstruction in children with recurrent upper airway infections

Bellodi¹,

Tosca²,

Pulvirenti³

et al. 2006

International Journal of Pediatric Otorhinolaryngology

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“…First, RVs initiate infections by first binding to cell surface receptors on airway epithelial cells. Notably, ICS can downregulate in vitro expression of intercellular adhesion molecule 1 (ICAM-1), which is the cellular receptor for 89 of the 100 canonical RV-A and RV-B types [7,8]; however, glucocorticoid treatment did not prevent the increased ICAM-1 expression after experimental RV-16 infection [9]. Second, RVs cause illnesses in part by inducing release of pro-inflammatory chemokines and cytokines from infected epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

Budesonide and formoterol effects on rhinovirus replication and epithelial cell cytokine responses

Bochkov¹,

Busse²,

Brockman-Schneider³

et al. 2013

Respiratory Research

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BackgroundCombination therapy with budesonide and formoterol reduces exacerbations of asthma, which are closely associated with human rhinovirus (RV) infections in both children and adults. These data suggest that budesonide and formoterol inhibit virus-induced inflammatory responses of airway epithelial cells.MethodsTo test this hypothesis, bronchial epithelial (BE) cells were obtained from airway brushings of 8 subjects with moderate-to-severe allergic asthma and 9 with neither asthma nor respiratory allergies. Cultured BE cells were incubated for 24 hours with budesonide (1.77 μM), formoterol (0.1 μM), both, or neither, and then inoculated with RV-16 (5×106 plaque forming units [PFU]/mL). After 24 hours, viral replication (RV RNA), cytokine secretion (CXCL8, CXCL10, TNFα, IFN-β, IL-28) and mRNA expression (CXCL8, CXCL10, TNF, IFNB1, IL28A&B) were analyzed.ResultsRV infection induced CXCL10 protein secretion and IFNB1 and IL28 mRNA expression. Drug treatments significantly inhibited secretion of CXCL10 in mock-infected, but not RV-infected, BE cells, and inhibited secretion of TNFα under both conditions. Neither budesonide nor formoterol, alone or in combination, significantly affected viral replication, nor did they inhibit RV-induced upregulation of IFNB1 and IL28 mRNA. Overall, RV replication was positively related to CXCL10 secretion and induction of IFNB1 and IL28 mRNA, but the positive relationship between RV RNA and CXCL10 secretion was stronger in normal subjects than in subjects with asthma.ConclusionsBudesonide and formoterol can inhibit BE cell inflammatory responses in vitro without interfering with viral replication or production of interferons. These effects could potentially contribute to beneficial effects of budesonide/formoterol combination therapy in preventing RV-induced asthma exacerbations.

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“…DSCG, Dex, and Fp have been reported to attenuate ICAM-1 in vivo or in vitro (15)(16)(17). In this study, DSCG attenuated ICAM-1 expression induced by RSV infection more strongly than corticosteroids.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that ICAM-1 expressed by airway epithelial cells is one of the major NTHi receptors (8,14), and RSV infection up-regulates ICAM-1 expression by A549 cells (8). Previous studies have revealed that Dex-or Fp-attenuated cytokine-induced ICAM-1 expression in human airway epithelial cells in vitro (15,16) and ICAM-1 expression in the bronchial epithelium was inhibited after treatment with inhaled DSCG in patients with bronchial asthma in vivo (17).…”

Section: Methodsmentioning

confidence: 99%

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The Effects of Disodium Cromoglycate on Enhanced Adherence of Haemophilus influenzae to A549 Cells Infected With Respiratory Syncytial Virus

et al. 2009

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Nontypeable Haemophilus influenzae (NTHi) secondary infection often complicates respiratory syncytial virus (RSV) infections. Previous studies have revealed that RSV infections enhance NTHi adherence to airway epithelial cells. In this study, we investigated the effects of disodium cromoglycate (DSCG) and corticosteroids, which are frequently used for the treatment of wheezing often related to RSV infections, on the adherence of NTHi to RSV-infected A549 cells. DSCG inhibited enhanced adherence of NTHi to RSV-infected A549 cells, whereas dexamethasone (Dex) and fluticasone propionate (Fp) did not. DSCG suppressed the expression of ICAM-1, which is one of the NTHi receptors. Furthermore, DSCG exhibited an inhibitory effect on RSV infections. It is suggested that DSCG exerts an anti-RSV effect, and consequently attenuates the expression of NTHi receptors. (Pediatr Res 66: 168-173, 2009) R espiratory syncytial virus (RSV) is one of the major pathogens of upper and lower respiratory tract infections in children. RSV infection at a younger age often involves the lower respiratory tract and is frequently associated with expiratory wheezing, which is referred to as bronchiolitis or wheezy bronchitis, asthma, and pneumonia (1). It is known that RSV infections can be complicated by bacterial superinfections (2-5). Nontypeable Haemophilus influenzae (NTHi) is one of the most common bacteria involved in mixed RSVbacterial bronchopulmonary infections in pediatric patients (2,4). It has long been recognized that a preceding local respiratory viral infection seems to play an important role in the pathogenesis of infections by bacteria, including NTHi. The mechanisms underlying bacterial superinfections include virus-induced local destruction of the epithelium, which compromises the host's physiologic barrier, and virus-induced modulation of the immune response (6). In addition, enhanced bacterial adherence to virus-infected cells is considered an important factor increasing the risk of bacterial superinfections (7).Recent studies demonstrated that some respiratory viruses including RSV lead to both expression of viral glycoproteins and up-regulation of cellular molecules including ICAM-1 (CD54), carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), and platelet activating factor receptor (PAFr) on the host-cell membrane. Both could serve as bacterial receptors and promote bacterial adhesion to the cells (8 -11). Strategies for preventing interaction between RSV and bacteria may reduce the incidence of secondary bacterial complications of RSV infection.Disodium cromoglycate (DSCG) and corticosteroids, which are recognized as inhalation drugs for the management of bronchial asthma, are also used for the treatment of acute infantile wheezing and exacerbation of asthma that are often related to RSV infections. The effects of these medicines against RSV infections and secondary bacterial complications are not clear. In this study, we investigated the effects of DSCG and corticosteroids on the in vitro inte...

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Modulation of the constitutive or cytokine-induced bronchial epithelial cell functions in vitro by fluticasone propionate

Cited by 6 publications

References 34 publications

Activity of budesonide on nasal neutrophilic inflammation and obstruction in children with recurrent upper airway infections

Activity of budesonide on nasal neutrophilic inflammation and obstruction in children with recurrent upper airway infections

Budesonide and formoterol effects on rhinovirus replication and epithelial cell cytokine responses

The Effects of Disodium Cromoglycate on Enhanced Adherence of Haemophilus influenzae to A549 Cells Infected With Respiratory Syncytial Virus

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