Modulation of the intestinal microbiota and the metabolites produced by the administration of ice cream and a dietary supplement containing the same probiotics

Abstract

The aim of the present work was to compare the capacity to modulate the intestinal microbiota and the production of metabolites after 14 d administration of a commercial dietary supplement and a manufactured ice cream, both containing the same quantity of inulin and the same viable counts of Lactobacillus acidophilus LA-5 and Bifidobacterium animalis BB-12, using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®) model. Samples of the colonic contents were evaluated microbiologically by real-time quantitative PCR (qRT-PCR) and next-generation sequencing and chemically by the production of SCFA (acetate, propionate and butyrate) and ammonium ions ($\text{NH}_4^ + $). Statistical analyses were carried out for all the variables using the two-way ANOVA followed by the Tukey multiple comparisons test (P < 0·05) for metabolite production, qRT-PCR and the bioinformatics analysis for microbiota diversity. Dietary supplement and ice cream were able to deliver the probiotic L. acidophilus and B. animalis to the simulated colon and modulate the microbiota, increasing beneficial micro-organisms such as Bifidobacterium spp., Bacteroides spp. and Faecalibacterium spp. for dietary supplement administration, and Lactobacillus spp. for ice cream supplementation. However, the ice cream matrix was probably more favourable for the maintenance of the metabolic activity of the probiotics in the SHIME® model, due to the larger amounts of acetate, propionate, butyrate and ammonium ions obtained after 14 d of supplementation. In conclusion, both ways of probiotic supplementation could be efficient, each with its own particularities.

Human microbiota modulation via QseC sensor kinase mediated in the Escherichia coli O104:H4 outbreak strain infection in microbiome model

“…The QseC sensor kinase role in intestinal microbiota composition was verified during the Shiga toxin producing O104:H4 E.coli infection. The C227–11 and C227–11 ::qseC strain were employed during in vitro infection of controlled colonic SHIME® model, to mimic the human gastrointestinal tract, evaluating the intestinal microbiota abundance through C227–11 infection [ 49 ], as detailed illustrated (Fig. 1 a and b).…”

“…The reactor was adapted for this study, where the transverse and descending colon were replaced by the triplicate of the ascending colon (pH 5.6–5.9), aiming to obtain replicates of the experiment for statistical comparison of the data (Fig. 1 a), with experimental settings as previously described [ 49 ].…”

“…The compartments were colonized with feces earlier collected and stored from three healthy volunteers with ages between 18 and 22 years old, according to the procedures [ 77 ], adapted to this study [ 78 ], and sampling prepared as previously described [ 49 ]. All feeding components (Sigma Aldrich, USA) are listed in Table 2 .…”

“…A 2 weeks period of stabilization was performed and the first treatment was administered for 72 h. The first infection consisted of 10 10 CFU/mL of E. coli C227–11 strain, 240 mL of feed medium and pancreatic juice (60 mL) were added. Between the treatments, a 72 h washout period was performed [ 49 ]. After the washout, under similar conditions during the second infection (72 h) started with the 10 10 CFU/mL E. coli C227–11:: qseC strain in the same conditions, all the experiments were performed in biological triplicates.…”

Immune status, well-being and gut microbiota in military supplemented with synbiotic ice cream and submitted to field training: a randomised clinical trial

“…A study, recently published by our group, using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®) showed that synbiotic ice cream produced with Bifidobacterium animalis subsp. lactis BB-12 and Lactobacillus acidophilus LA-5 and inulin favoured the maintenance and metabolic activity of probiotics added, due to the increase in the proportion of SCFA (acetate, propionate and butyrate) (19) .…”