Modulation of Tumor Cell Metabolism by Laser Photochemotherapy with Cisplatin or Zoledronic Acid In Vitro

Heymann, Paul; Henkenius, Katharina; Ziebart, Thomas; Braun, Andreas; Hirthammer, Klara; Halling, Frank; Neff, Andreas; Mandic, Robert

doi:10.21873/anticanres.12351

Cited by 7 publications

(4 citation statements)

References 41 publications

(49 reference statements)

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“…Previous work by Heymann and colleagues reported a PBM-induced decrease in the redox ratio, measured by the extra-cellular flux assay, along with increased proliferation in HeLa cells, using 670 nm and 12 J/cm 2 (39). Since the illumination protocol and cell type were different, but the effect similar, it might be a common effect of PBM in tumors.…”

Section: Discussionmentioning

confidence: 99%

Effects of photobiomodulation on the redox state of healthy and cancer cells

Faria

Ciol

Bagnato

et al. 2020

Preprint

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Photobiomodulation (PBM) uses light to stimulate cells. The molecular basis of the effects of PBM is being unveiled, but it is stated that the cytochrome-c oxidase enzyme in mitochondria, a photon acceptor of PBM, contributes to an increase in ATP production and modulates the reduction and oxidation of electron carriers NADH and FAD. As it can stimulate cells, PBM is not used on tumors. Thus, it is interesting to investigate if its effects correlate to mitochondrial metabolism and if so, how it could be linked to the optical redox ratio (ORR). To that end, fibroblasts and oral cancer cells were irradiated with a light source of 780 nm and a total dose of 5 J/cm2, and imaged by optical microscopy. PBM down-regulated the SCC-25 ORR by 10%. Furthermore, PBM led to an increase in ROS and ATP production in cancer cells after 4 h, while fibroblasts only had a modest ATP increase 6 h after irradiation. Cell lines did not show distinct cell cycle profiles, as both had an increase in G2/M cells. This study indicates that PBM shifts the redox state of oral cancer cells towards glycolysis and affects normal and tumor cells through distinct pathways. To our knowledge, this is the first study that investigated the effects of PBM on mitochondrial metabolism from the initiation of the cascade to DNA replication. This is an essential step in the investigation of the mechanism of action of PBM in an effort to avoid misinterpretations of a variety of combined protocols.

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Section: Discussionmentioning

confidence: 99%

Effects of photobiomodulation on the redox state of healthy and cancer cells

Faria

Ciol

Bagnato

et al. 2020

Preprint

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“…Furthermore, when administered as adjuvant therapy added on the standard therapy, ZOL has been shown to reduce bone metastasis in breast cancer patients with a high risk of bone metastases (22). The effect of ZOL on bone lesions has been widely reported (23)(24), but its effect on the local tumor itself has been questioned in some areas (19,25). In this study, we used a new bone metastasis model to investigate the effects of ZOL on tumor lesions and bone metastatic lesions.…”

Section: Discussionmentioning

confidence: 99%

Development of New Mouse Breast Cancer Model of Local Bone Metastasis and Verification Using Bisphosphonates

Shoji

Tsuchie

Nagasawa

et al. 2022

In Vivo

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Background/Aim: Local tumor injection models require complicated procedures. The purpose was to establish a simple local bone metastasis model using normal mice, and to study the usefulness of the model with bisphosphonates (BP). Materials and Methods: This study used a versatile C57BL/6 mouse model and E0771 cells. Tumor cells were injected into the right femur. Mice were divided into groups depending on the concentration of cells injected and the use of BP or not. The degree of bone destruction between the different conditions was compared using micro-computed tomography (μCT). Results: Bone destruction was confirmed in four mice in the highconcentration group at 3 weeks, and in all other mice at 4 and 6 weeks. At 6 weeks post-injection, bone destruction was significantly suppressed in the BP group (p<0.05). Conclusion: We created a breast cancer mouse model of local bone metastasis. Zoledronate showed the same usefulness as in previous models. It may be an effective model for evaluating treatments for bone metastasis. Materials and MethodsCell culture. E0771 (CH3 Biosystems LLC, NY) cells were cultured in Dulbecco's modified Eagle's medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (Mediatech, Manassas, VA, USA) and 100 μg/ml kanamycin sulfate (Meiji Seika Pharma, Tokyo, Japan). These were maintained in a humidified atmosphere of 5% CO 2 in air and 37˚C (14). The cells were verified 667 This article is freely accessible online.

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“…Thus, our results may reveal that the two pathways interact with each other in the process of LLLT promoting hair growth. Besides, LLLT has been reported to increase metabolism of cell lines such as HeLa cells in the past 29 . Our results also found that its effect of promoting hair growth is related to the metabolic pathway and those metabolites can be produced or decomposed from acid degradation and lysosome pathways.…”

Section: Discussionmentioning

confidence: 99%