Modulatory Effect of Competitive Exclusion on the Transmission of ESBL E. coli in Chickens

Ebrahem, Amera F.; El-Demerdash, Azza S.; Orady, Rania M.; Nabil, Nehal M.

doi:10.1007/s12602-023-10095-1

Cited by 4 publications

(1 citation statement)

References 54 publications

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“…Several recent studies have reported the emergence of multidrug-resistant bacterial pathogens from different origins necessitating proper use of antibiotics. Routine application of antimicrobial susceptibility testing is necessary to detect the antibiotic of choice and to screen for the emerging MDR strains (El-Demerdash et al 2018 ; Algammal et al 2021 , 2022a , 2023 ; Ebrahem et al 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Pathognomonic features of Pasteurella multocida isolates among various avian species in Sharkia Governorate, Egypt

El-Demerdash,

Mowafy,

Fahmy

et al. 2023

World J Microbiol Biotechnol

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The present study aimed to isolate Pasteurella multocida (P. multocida) from pulmonary cases in several avian species and then investigate the histopathological features, antimicrobial resistance determinants, virulence characteristics, and risk factors analysis of the isolates in each species in correlation with epidemiological mapping of pasteurellosis in Sharkia Governorate, Egypt. The obtained data revealed a total occurrence of 9.4% (30/317) of P. multocida among the examined birds (chickens, ducks, quails, and turkeys). The incidence rate was influenced by avian species, climate, breed, age, clinical signs, and sample type. Antimicrobial susceptibility testing revealed that all isolates were sensitive to florfenicol and enrofloxacin, while 86.6 and 73.3% of the isolates displayed resistance to amoxicillin-clavulanic acid and erythromycin, respectively. All of the P. multocida isolates showed a multiple-drug resistant pattern with an average index of 0.43. Molecular characterization revealed that the oma87, sodA, and ptfA virulence genes were detected in the all examined P. multocida isolates. The ermX (erythromycin), blaROB-1 (β-lactam), and mcr-1(colistin) resistance genes were present in 60, 46.6, and 40% of the isolates, respectively. Ducks and quails were the most virulent and harbored species of antimicrobial-resistant genes. These results were in parallel with postmortem and histopathological examinations which detected more severe interstitial pneumonia lesions in the trachea and lung, congestion, and cellular infiltration especially in ducks. Epidemiological mapping revealed that the Fakous district was the most susceptible to pasteurellosis infection. Thus, farmers are recommended to monitor their flocks for signs of respiratory disease, seek veterinary care promptly if any birds are sick, and avoid the random usage of antibiotics. In conclusion, this study presents a comprehensive picture of the risk factors in correlation to the pathognomonic characteristics of P. multocida infection in poultry sectors to help in developing more effective strategies for prevention and control.

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Section: Introductionmentioning

confidence: 99%

Pathognomonic features of Pasteurella multocida isolates among various avian species in Sharkia Governorate, Egypt

El-Demerdash,

Mowafy,

Fahmy

et al. 2023

World J Microbiol Biotechnol

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An Alternative Approach Using Nano-garlic Emulsion and its Synergy with Antibiotics for Controlling Biofilm-Producing Multidrug-Resistant Salmonella in Chicken

El-Demerdash,

Orady,

Matter

et al. 2023

Indian J Microbiol

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Surface-growing antibiotic-resistant pathogenic Salmonella is emerging as a global health challenge due to its high economic loss in the poultry industry. Their pathogenesis, increasing antimicrobial resistance, and biofilm formation make them challenging to treat with traditional therapy. The identification of antimicrobial herbal ingredients may provide valuable solutions to solve this problem. Therefore, our aim is to evaluate the potency of nano garlic as the alternative of choice against multidrug-resistant (MDR) Salmonella isolates using disc diffusion and microdilution assays. Then, checkerboard titration in trays was applied, and FIC was measured to identify the type of interaction between the two antimicrobials. A disc diffusion assay revealed that neomycin was the drug of choice. The range of nano garlic MIC was 12.5–25 μg/ml, while the neomycin MIC range was 32–64 μg/ml. The FIC index established a synergistic association between the two tested drugs in 85% of isolates. An experimental model was used including nano garlic and neomycin alone and in combination against Salmonella infection. The combination therapy significantly improved body productivity and inhibited biofilm formation by more than 50% down regulating the CsgBAD, motB, and sipA operons, which are responsible for curli fimbriae production and biofilm formation in Salmonella serotypes.

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The development of antimicrobial resistance in broilers affected by veterinary antimicrobials and a probiotic administration

Tiurina,

Gorfunkel,

Filippova

et al. 2024

Agrarnaâ nauka

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Relevance. The broad use of antimicrobials by agriculture and consumers motivation to buy antibiotic-free production form a basis to development of substitutes to antimicrobials. The goal of research was the analysis of antimicrobial resistance caused by veterinary antimicrobials administration to broilers, as well as the effect of metaprobiotic “Probiocid-Ultra” on antimicrobial resistance in dynamic.Methods. Three groups of broiler chickens were formed: I — control, who received the main diet, II — experimental, who received veterinary antibiotics enrofloxacin and colistin in addition to the diet of group I, III — experimental, who received the metaprobiotic “Probiocide-Ultra” in addition to the diet of group II. Gene expression analysis was performed using quantitative reverse transcription PCR. With inoculation to different media the antimicrobial resistance was examined with classical microbiological methods.Results. The dynamic of accumulation of antimicrobial resistance determinants caused by enrofloxacin and colistin administration to broilers was examined. Under the influence of enrofloxacin the increase in antimicrobial resistance to enrofloxacin itself, as well as to beta-lactams, tetracycline and colistin was observed. Under the influence of colistin the increase in antimicrobial resistance to colistin itself, as well as to enrofloxacin and beta-lactams was observed. The feed administration with metaprobiotic “Probiocid-Ultra” promoted noticeable decrease of the antimicrobial resistance genes amount, when analyzed with growth medium led to antimicrobial resistant enterobacteria vanished.

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Modulatory Effect of Competitive Exclusion on the Transmission of ESBL E. coli in Chickens

Cited by 4 publications

References 54 publications

Pathognomonic features of Pasteurella multocida isolates among various avian species in Sharkia Governorate, Egypt

Pathognomonic features of Pasteurella multocida isolates among various avian species in Sharkia Governorate, Egypt

An Alternative Approach Using Nano-garlic Emulsion and its Synergy with Antibiotics for Controlling Biofilm-Producing Multidrug-Resistant Salmonella in Chicken

The development of antimicrobial resistance in broilers affected by veterinary antimicrobials and a probiotic administration

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