Modulatory effect of grape-seed procyanidins on local and systemic inflammation in diet-induced obesity rats

Terra, Ximena; Pallarès, Víctor; Ardèvol, Anna; Bladé, Cinta; Fernández-Larrea, Juan; Pujadas, Gerard; Salvadó, Josepa; Arola, Lluı́s; Blay, Mayte

doi:10.1016/j.jnutbio.2010.03.006

Cited by 144 publications

(125 citation statements)

References 31 publications

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“…Metabolic syndrome was induced by offering the animals a very tasty HFD that they could eat ad libitum. Other studies by our research group on the same animals, whose liver proteome has been studied here, have shown that HFDfed rats suffer from metabolic syndrome symptoms such as obesity, fatty liver due to hepatic TG and cholesterol accumulation, dyslipidemia caused by high plasma TG and low density lipoprotein cholesterol (25), hyperinsulinemia (16), and inflammation (88). All these symptoms were slightly reduced in the group of HFD-fed rats treated with GSPE for 10 days (HFD ϩ GSPE group) except dyslipidemia, which was completely corrected.…”

Section: Discussionmentioning

confidence: 67%

Lipogenesis Is Decreased by Grape Seed Proanthocyanidins According to Liver Proteomics of Rats Fed a High Fat Diet

Baiges

Palmfeldt

Bladé

et al. 2010

Molecular & Cellular Proteomics

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Bioactive proanthocyanidins have been reported to have several beneficial effects on health in relation to metabolic syndrome, type 2 diabetes, and cardiovascular disease. We studied the effect of grape seed proanthocyanidin extract (GSPE) in rats fed a high fat diet (HFD). This is the first study of the effects of flavonoids on the liver proteome of rats suffering from metabolic syndrome. Three groups of rats were fed over a period of 13 weeks either a chow diet (control), an HFD, or a high fat diet supplemented for the last 10 days with GSPE (HFD ؉ GSPE). The liver proteome was fractionated, using a Triton X-114-based two-phase separation, into soluble and membrane protein fractions so that total proteome coverage was considerably improved. The data from isobaric tag for relative and absolute quantitation (

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Section: Discussionmentioning

confidence: 67%

Lipogenesis Is Decreased by Grape Seed Proanthocyanidins According to Liver Proteomics of Rats Fed a High Fat Diet

Baiges

Palmfeldt

Bladé

et al. 2010

Molecular & Cellular Proteomics

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“…The composition of the diet for each experimental group is shown in Table 1. Standard laboratory diet included 12% calories as fat with an energy density of 3.50 kcal/g and HFD contained 54% calories as fat with an energy density of 4.90 kcal/g [Terra et al, 2011]. The diets were given in the form of pellets.…”

Section: Animal Model and Experimental Designmentioning

confidence: 99%

Effects of Silybum marianum Extract on High-Fat Diet Induced Metabolic Disorders in Rats

Sayın¹,

Büyükbaş²,

Başaralı³

et al. 2016

Pol. J. Food Nutr. Sci.

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“…In this context, our group and others have reported many healthy beneficial effects of a grape seed procyanidin extract (GSPE) (rich in monomers and oligomers of flavan-3-ols, the most abundant polyphenols in the human diet), on different pathologies clustered within the metabolic syndrome, such as insulin resistance, dyslipidaemia, obesity, hypertension and inflammation (Del Bas et al 2005;Pinent et al 2006;Quesada et al 2009;Bladé et al 2010;Terra et al 2011;Belcaro et al 2013;Caimari et al 2013). Interestingly, some studies have shown that polyphenols, which may be isolated or included in extracts or foods, such as olive oil and cocoa, are also able to regulate the gene expression profiles of PBMCs (Konstantinidou et al 2009;Khymenets et al 2009;Camargo et al 2010;Crescenti et al 2013;Tomé-Carneiro et al 2013;van der Velpen et al 2013).…”

Section: Introductionmentioning

confidence: 99%

The intake of a high-fat diet and grape seed procyanidins induces gene expression changes in peripheral blood mononuclear cells of hamsters: capturing alterations in lipid and cholesterol metabolisms

et al. 2014

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We previously demonstrated that hamsters that were fed either a standard diet (STD) or a high-fat diet (HFD) and treated with a grape seed procyanidin extract (GSPE) showed decreased adiposity and circulating levels of free fatty acids compared with hamsters treated with a vehicle (Caimari et al. in Int J Obes 37:576-83, 2013, doi:10.1038/ijo.2012). Here, we tested whether the gene expression changes in peripheral blood mononuclear cells (PBMCs) can reflect these metabolic effects and the dyslipidaemia produced by the HFD feeding in the same cohort of animals. The mRNA levels of a subset of genes were also studied in the liver in order to evaluate the capacity of PBMCs to reflect the metabolic adaptations that occur in this organ. In PBMCs, we reported a simultaneous up-regulation of the lipid-related genes involved in both the anabolic (pparc, acc1 and gpat) and the catabolic (ppara, ucp2, atgl and hsl) pathways in response to the GSPE treatment, similar but no identical to previous observations in retroperitoneal white adipose tissues of these animals. Furthermore, the key cholesterol metabolism genes srebp2 and ldlr were significantly down-regulated in PBMCs of both HFD-fed groups compared with the STD groups. Although the expression of srebp2 in the liver followed a similar pattern to that obtained in PBMCs, no comparable changes were found between the liver and PBMCs in the expression of most of the studied genes. In conclusion, our results highlight the potential of PBMCs as a high accessible tissue for the indirect study of cholesterol and adipose tissue metabolism dynamics.

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Modulatory effect of grape-seed procyanidins on local and systemic inflammation in diet-induced obesity rats

Cited by 144 publications

References 31 publications

Lipogenesis Is Decreased by Grape Seed Proanthocyanidins According to Liver Proteomics of Rats Fed a High Fat Diet

Lipogenesis Is Decreased by Grape Seed Proanthocyanidins According to Liver Proteomics of Rats Fed a High Fat Diet

Effects of Silybum marianum Extract on High-Fat Diet Induced Metabolic Disorders in Rats

The intake of a high-fat diet and grape seed procyanidins induces gene expression changes in peripheral blood mononuclear cells of hamsters: capturing alterations in lipid and cholesterol metabolisms

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