Modulatory effect of hydrogen peroxide on tumoral lymphocytes proliferation

Davicino, Roberto; Manuele, María Gabriela; Ferraro, Graciela; Micalizzi, Blas; Anesini, Claudia

doi:10.1080/08923970802520701

Cited by 18 publications

(27 citation statements)

References 25 publications

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“…It is already known that the activation of macrophages induces an increase in molecules which can conduce cells to apoptosis; among these molecules, TNF-α and H 2 O 2 can be mentioned. In this respect, it was demonstrated that H 2 O 2 induces lymphocytes to late and early apoptosis, (19) also TNF-α is capable of producing cell death by apoptosis. (20) Activation of macrophages phagocytosis was also observed with other compounds and plant extracts.…”

Section: Discussionmentioning

confidence: 98%

“In vivo” murine macrophages activation by a dichloromethane extract ofTilia x viridis

Davicino¹,

Micucci²,

Zettler³

et al. 2010

Immunopharmacology and Immunotoxicology

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Macrophages are involved in the host defense against infectious pathogens and tumors. Tilia species have been used in folk medicine for the treatment of infectious diseases, previously it was demonstrated that a dichloromethane (DM) extract possess antiproliferative action "in vitro" on a lymphoma cell line. The aim of this work was to study the "in vivo" effect of DM extract upon mice peritoneal macrophages. DM extract-activated macrophages phagocytosis through hydrogen peroxide (H(2)O(2)) and nitric oxide (NO) production (phagocytosis (%): basal 16.93 +/- 0.18, DM extract 25.93 +/- 2.8; H(2)O(2) (M): basal 0.0022 +/- 0.00016, DM extract 0.0036 +/- 0.0005; NO (mM): basal 0.0052 +/- 0.0007, DM extract 0.0099 +/- 0.0004). These actions were mediated by cell superoxide dismutase activation. On the other hand, DM extract decreased tumor necrosis factor alpha but increased interleukin-10 in serum. These results suggest that the modulation activity exerted by the extract on immune system cells could be an important mechanism to acquire resistance to tumors and infectious diseases.

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Section: Discussionmentioning

confidence: 98%

“In vivo” murine macrophages activation by a dichloromethane extract ofTilia x viridis

Davicino¹,

Micucci²,

Zettler³

et al. 2010

Immunopharmacology and Immunotoxicology

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“…In normal lymphocytes, however, the antiproliferative action exerted by the extract appeared to be related to peroxidase activity, as the EC 50 values for both effects were in the same range. It has been demonstrated previously that normal lymphocytes contain more H 2 O 2 than BW 5147 cells under basal conditions (Davicino et al , ); these higher concentrations of H 2 O 2 may therefore allow the cells to proliferate so that when the extract exerted peroxidase activity, scavenging H 2 O 2 , an inhibition of cell proliferation occurred. There is evidence indicating that H 2 O 2 and the superoxide anion can influence the growth and death of cells.…”

Section: Discussionmentioning

confidence: 98%

“…It has been shown previously that BW 5147 cells maintain low levels of H 2 O 2 so peroxidase activity could not contribute to the antiproliferative effect, as shown in this study. Moreover, an increase of H 2 O 2 contributed to the antiproliferative action of an extract of Larrea divaricata in the same cell line (Davicino et al , ). So in BW 5147 cells, it is an increase in H 2 O 2 that produces inhibition of cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

HPLC Fingerprint of a Flower Extract of Tilia × viridis and Correlation with Antiproliferative and Antioxidant Activity

Marrassini

Anesini

Ferraro

2011

Phytotherapy Research

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Tilia species have been used in Europe and in America to treat anxiety and also for the treatment of colds, influenza, bronchitis, fever and inflammation. Tilia × viridis is a Tilia species distributed widely in Buenos Aires, Argentina. The flavonoids present in Tilia species have antioxidant properties, acting as reactive oxygen species (ROS) scavengers, principally on hydrogen peroxide (H(2)O(2)) and the superoxide anion (O(2)(·-)), which are involved in many diseases, including cancer. The aim of this study was to determine the phytochemical pattern of the ethanol extract of Tilia × viridis, principally the flavonoid content, and to evaluate the antiproliferative effects on both normal and tumoral cells, and the antioxidant activity in relation to H(2)O(2) modulation. The extract was found to present a selective antiproliferative activity on a lymphoma cell line and this was related to free radical scavenging activity. In addition, one of its main compounds, rutin, showed antioxidant effects related to peroxidase activity. T. × viridis may therefore be a source of antioxidant compounds that contribute to a selective antiproliferative action on tumoral cells, acting by modulation of H(2)O(2) levels.

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“…MTT is a membrane‐permeable tetrazolium salt, which is reduced to formazan by superoxide anion in living cells, 18,23 either in the mitochondria or in intracellular vesicles identified as endosomes and lysosomes 24 . We have already demonstrated that L. divaricata decoction produces mitochondrial superoxide 10 . We believe that the high levels of reduced MTT could be indicating an increased production of superoxide anion 25 …”

Section: Discussionmentioning

confidence: 98%

“…7,8 Moreover, fractions isolated from the decoction induced the activation of the innate immune system in vitro. 9 On the other hand, Davicino et al [10] showed the effect of an aqueous extract of this plant on proliferation, apoptosis, activation of Mn 2+ -dependent superoxide dismutase, and production of H 2 O 2 and nitric oxide in the tumour cell line BW5147.…”

Section: Introductionmentioning

confidence: 99%

In vivo effect of three fractions of Larrea divaricata Cav. (jarilla) on the innate immune system: macrophage response against Candida albicans

Martino

Davicino

Mattar

et al. 2011

Mycoses

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Larrea divaricata Cav. (jarilla) is a plant with well-documented applications in folk medicine in Argentina. In this study, we aimed to evaluate functional parameters of peritoneal macrophages isolated from mice injected with three fractions (F1, F2 and F3) of L. divaricata. The response of macrophages against Candida albicans was evaluated. Cell viability was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, apoptosis was evaluated using Giemsa, acridine orange/ethidium bromide and ladder assay, oxidative burst was assayed using nitroblue tetrazolium test and nitrite production using Griess assay. Cell stimulation and their ability to kill C. albicans in vitro were measured. The number and cell viability were similar to controls. However, we found that F1 induces pre-activation of macrophages, and this pre-activation is enhanced by C. albicans. The effects exerted by F1 make it more important than F2 and F3 for the treatment of disseminated candidiasis in patients with immunodeficiency diseases such as AIDS and chronic granulomatous disease, among others.

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Modulatory effect of hydrogen peroxide on tumoral lymphocytes proliferation

Cited by 18 publications

References 25 publications

“In vivo” murine macrophages activation by a dichloromethane extract ofTilia x viridis

“In vivo” murine macrophages activation by a dichloromethane extract ofTilia x viridis

HPLC Fingerprint of a Flower Extract of Tilia × viridis and Correlation with Antiproliferative and Antioxidant Activity

In vivo effect of three fractions of Larrea divaricata Cav. (jarilla) on the innate immune system: macrophage response against Candida albicans

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