Modulatory effect of N-acetylcysteine on pro-antioxidant status and haematological response in healthy men

Zembroń-Łacny, Agnieszka; Słowińska-Lisowska, Małgorzata; Szyguła, Zbigniew; Witkowski, Z.; Szyszka, K

doi:10.1007/s13105-010-0002-1

Cited by 29 publications

(37 citation statements)

References 24 publications

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“…Aggregation of lipids in aqueous solutions results in marked effects on the light scattering, thus interfering with the reliability of the spectrometric determination of the employed biomarker. These considerations are of course valid for determination of most if not all of the different biomarkers by commercial kits [51][52][53][54][55], because the user may not always conduct concentration-dependent measurements, even when recommended in the manual.…”

Section: Methodsmentioning

confidence: 99%

The relationship between oxidative stress and exercise

Finkler

Lichtenberg

Pinchuk

2013

Journal of Basic and Clinical Physiology and Pharmacology

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Physical exercise has many benefits, but it might also have a negative impact on the body, depending on the training level, length of workout, gender, age and fitness. The negative effects of physical exercise are commonly attributed to an imbalance between the levels of antioxidants (both low molecular weight antioxidants and antioxidant enzymes) and reactive oxygen and nitrogen species due to excessive production of free radicals during physical exercise. In this critical review, we look for answers for three specific questions regarding the interrelationship between physical exercise and oxidative stress (OS), namely, (i) the dependence of the steady-state level of OS on fitness, (ii) the effect of intensive exercise on the OS and (iii) the dependence of the effect of the intense exercise on the individual fitness. All these questions have been raised, investigated and answered, but the answers given on the basis of different studies are different. In the present review, we try to explain the reason(s) for the inconsistencies between the conclusions of different investigations, commonly based on the concentrations of specific biomarkers in body fluids. We think that most of the inconsistencies can be attributed to the difference between the criteria of the ill-defined term denoted OS, the methods used to test them and in some cases, between the qualities of the applied assays. On the basis of our interpretation of the differences between different criteria of OS, we consider possible answers to three well-defined questions. Possible partial answers are given, all of which lend strong support to the conclusion that the network responsible for homeostasis of the redox status is very effective. However, much more data are required to address the association between exercise and OS and its dependence on various relevant factors.

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Section: Methodsmentioning

confidence: 99%

The relationship between oxidative stress and exercise

Finkler

Lichtenberg

Pinchuk

2013

Journal of Basic and Clinical Physiology and Pharmacology

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“…ZembronLacny et al (2010) observed that a daily dose of 1,200 mg of N-acetylcysteine (NAC) affects hematological parameters in healthy men. NAC increased the plasma levels of Epo, Hb, and Hct at rest and after exercise (Zembron-Lacny et al 2010). On the other hand, the authors showed that LPO was reduced by NAC by more than 30% (Zembron-Lacny et al 2010).…”

Section: Vitamin C and Erythropoiesismentioning

confidence: 97%

“…NAC increased the plasma levels of Epo, Hb, and Hct at rest and after exercise (Zembron-Lacny et al 2010). On the other hand, the authors showed that LPO was reduced by NAC by more than 30% (Zembron-Lacny et al 2010). However, we did not find any significant modification in any hematological parameter or a reduction in the plasma protein and lipid oxidation after vitamin C supplementation in animals submitted to our IHE protocol.…”

Section: Vitamin C and Erythropoiesismentioning

confidence: 97%

Vitamin C Supplementation Does not Improve Hypoxia-Induced Erythropoiesis

Martínez-Bello

Sanchís-Gomar

Martinez‐Bello

et al. 2012

High Altitude Medicine & Biology

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a. Vitamin C Supplementation Does Not Improve Hypoxia-Induced Erythropoiesis. High Alt Med Biol 13:269-274, 2012.-Hypoxia induces reactive oxygen species production. Supplements with antioxidant mixtures can compensate for the decline in red cell membrane stability following intermittent hypobaric hypoxia by decreasing protein and lipid oxidation. We aimed to determine whether supplementation with vitamin C is implicated in the regulation of erythropoiesis and in the oxygen-carrying capacity of the blood, and also whether antioxidant supplementation prevents the oxidative stress associated to intermittent hypoxia. Twentyfour male Wistar rats were randomly divided into four experimental groups: normoxia control (n = 6), normoxia + vitamin C (n = 6), hypoxia control (12 h pO 2 12%/12 h pO 2 21%) (n = 6), and hypoxia + vitamin C (n = 6). Animals were supplemented with vitamin C at a dose of 250 mg$kg -1 $day -1 for 21 days. Red blood cell count, hemoglobin, hematocrit, reticulocytes, erythropoietin, and oxidative stress parameters such as malondialdehyde and protein oxidation in plasma were analyzed at two different time points: basal sample (day zero) and final sample (day 21). Similar RBC, Hb, Hct, and Epo increments were observed in both hypoxic groups regardless of the vitamin C supplementation. There was no change on MDA levels after intermittent hypoxic exposure in any experimental group. However, we found an increase in plasma protein oxidation in both hypoxic groups. Vitamin C does not affect erythropoiesis and protein oxidation in rats submitted to intermittent hypoxic exposure.

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“…However, several chemically diverse antioxidants also can affect HIF-1 activity and EPO expression [11, 15, 18, 27, 31, 37, 38]. α-Lipoic acid is a very interesting antioxidant because of its anti- and pro-oxidative activity.…”

Section: Introductionmentioning

confidence: 99%

The COMBINATION OF Α-Lipoic ACID INTAKE WITH ECCENTRIC EXERCISE MODULATES ERYTHROPOIETIN RELEASE

Morawin¹,

Turowski²,

Naczk³

et al. 2014

Biol Sport

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The generation of reactive nitrogen/oxygen species (RN/OS) represents an important mechanism in erythropoietin (EPO) expression and skeletal muscle adaptation to physical and metabolic stress. RN/OS generation can be modulated by intense exercise and nutrition supplements such as α-lipoic acid, which demonstrates both anti- and pro-oxidative action. The study was designed to show the changes in the haematological response through the combination of α-lipoic acid intake with running eccentric exercise. Sixteen healthy young males participated in the randomised and placebo-controlled study. The exercise trial involved a 90-min run followed by a 15-min eccentric phase at 65% VO2max (-10% gradient). It significantly increased serum concentrations of nitric oxide (NO), hydrogen peroxide (H2O2) and pro-oxidative products such as 8-isoprostanes (8-iso), lipid peroxides (LPO) and protein carbonyls (PC). α-Lipoic acid intake (Thiogamma: 1200 mg daily for 10 days prior to exercise) resulted in a 2-fold elevation of serum H2O2 concentration before exercise, but it prevented the generation of NO, 8-iso, LPO and PC at 20 min, 24 h, and 48 h after exercise. α-Lipoic acid also elevated serum EPO level, which highly correlated with NO/H2O2 ratio (r = 0.718, P < 0.01). Serum total creatine kinase (CK) activity, as a marker of muscle damage, reached a peak at 24 h after exercise (placebo 732 ± 207 IU · L-1, α-lipoic acid 481 ± 103 IU · L-1), and correlated with EPO (r = 0.478, P < 0.01) in the α-lipoic acid group. In conclusion, the intake of high α-lipoic acid modulates RN/OS generation, enhances EPO release and reduces muscle damage after running eccentric exercise.

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Modulatory effect of N-acetylcysteine on pro-antioxidant status and haematological response in healthy men

Cited by 29 publications

References 24 publications

The relationship between oxidative stress and exercise

The relationship between oxidative stress and exercise

Vitamin C Supplementation Does not Improve Hypoxia-Induced Erythropoiesis

The COMBINATION OF Α-Lipoic ACID INTAKE WITH ECCENTRIC EXERCISE MODULATES ERYTHROPOIETIN RELEASE

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