Modulatory Effect of Phytoestrogens and Curcumin on Induction of Annexin 1 in Human Peripheral Blood Mononuclear Cells and their Inhibitory Effect on Secretory Phospholipase A&lt;sub&gt;2&lt;/sub&gt;

Ahmad, Waqas; Kumolosasi, Endang; Jantan, Ibrahim; Jasamai, Malina; Salim, Elsayed I.

doi:10.4314/tjpr.v13i2.1

Cited by 7 publications

(5 citation statements)

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“…Castro-Caldas et al reported that dexamethasone and 17β-estradiol promoted ANXA1 secretion in acute T lymphoblastic leukemia cells, CCRF-CEM [28]. Ahmad et al showed that phytoestrogens induced the production of Annexin A1 in peripheral blood mononuclear cells (PBMC) [29]. However, the underlying mechanism of phytoestrogens that modulate ANXA1 and regulate these processes is still not fully understood.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of phytoestrogens in inducing cell death mediated by decreasing Annexin A1 in Annexin A1-knockdown leukemia cells

et al. 2020

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Background Phytoestrogens are plant compounds that are structurally similar to estrogen and that possess anti-cancer properties. Previous studies have reported that coumestrol, daidzein and genistein could induce cell death by reducing Annexin A1 protein in leukemic cell lines. Annexin A1 (ANXA1) is involved in cell progression, metastasis, and apoptosis in several types of cancer cells. The present study sought to investigate if the effects of phytoestrogens on apoptosis, cell cycle arrest and phagocytosis in ANXA1-knockdown leukemic cells are mediated through ANXA1 or occurred independently. Methods Transfection of ANXA1 siRNA was conducted to downregulate ANXA1 expression in Jurkat, K562 and U937 cells. Apoptosis and cell cycle assays were conducted using flow cytometry. Western blot was performed to evaluate ANXA1, caspases and Bcl-2 proteins expression. Phagocytosis was determined using hematoxylin and eosin staining. ResultsThe expression of ANXA1 after the knockdown was significantly downregulated in all cell lines. Genistein significantly induced apoptosis associated with an upregulation of procaspase-3, −9, and − 1 in Jurkat cells. The Bcl-2 expression showed no significant difference in Jurkat, K562 and U937 cells. Treatment with phytoestrogens increased procaspase-1 expression in Jurkat and U937 cells while no changes were detected in K562 cells. Flow cytometry analysis demonstrated that after ANXA1 knockdown, coumestrol and genistein caused cell cycle arrest at G2/M phase in selected type of cells. The percentage of phagocytosis and phagocytosis index increased after the treatment with phytoestrogens in all cell lines. Conclusion Phytoestrogens induced cell death in ANXA1-knockdown leukemia cells, mediated by Annexin A1 proteins. KeywordsAnnexin A1 . siRNA . Apoptosis . Caspase cascade . Cell cycle arrest . Phagocytosis . Human leukemic cell lines Abbreviations ANXA1 annexinA1 Annexin V-FITC annexin V-fluorescein PI propidium iodide RPMI-1640 Roswell Park Memorial Institute medium 1640 IMDM Iscove's modified Dulbecco's medium FBS fetal bovine serum siRNA small interfering RNA ER estrogen receptor * Endang Kumolosasi

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Section: Introductionmentioning

confidence: 99%

Evaluation of phytoestrogens in inducing cell death mediated by decreasing Annexin A1 in Annexin A1-knockdown leukemia cells

et al. 2020

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“…tion, DNA replication, cell transformation, ion channel formation, and mediation of cell proliferation, differentiation and apoptosis (7,9,10). Ahmad et al (2014) have shown that production of ANXA1 in PBMC can be induced by curcumin and phytoestrogens (11). ANXA1 has been vastly studied for its anti-inflammatory properties, but little is known about its anticancer effects, including its effect on leukemia.…”

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confidence: 99%

Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines

2018

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Recent studies suggest that annexin A1 (ANXA1) promotes apoptosis in cancerous cells. This study aims to investigate the effects of ANXA1 on apoptosis and cell cycle arrest in K562, Jurkat and U937 cells and peripheral blood mononu-clear cells (PBMC). Cells were treated with ANXA1 and cyclophosphamide prior to flow cytometry analysis for apoptosis and cell cycle arrest induction. At 2.5µM, ANXA1 induced significant apoptosis in K562 (p ≤ 0.001) and U937 (p ≤ 0.05) cells, with EC50 values of 3.6 and 3.8 µM, respectively. In Jurkat cells, induction was not significant (EC50, 17.0 µM). No significant apoptosis induction was observed in PBMC. ANXA1 caused cycle arrest in the G0/G1 phase in K562 and U937 cells with p ≤ 0.001 for both, and (p ≤ 0.01) for Jurkat cells. ANXA1 induced apoptosis and cycle arrest in the G0/G1 phase in K562 and U937 cells, causing only cell cycle arrest in Jurkat cells.

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“…Although, it has shown many excellent pharmacological activities in in vitro experiments such as antioxidant, antiinflammatory, anti-microbial and also anti-tumor activities [1][2][3][4], curcumin has not yet been approved for use as a drug due to the very low bioavailability obtained after curcumin oral administration, which is due, in part, to poor solubility (11 ng/mL in aqueous buffer, pH 5.5 [5]) and stability (t 1/2 of curcumin in PBS pH 7.2, < 10 min [6]). Many attempts have been made to solve these problems.…”

Section: Introductionmentioning

confidence: 99%

Curcumin-Hydroxypropyl-β-Cyclodextrin Inclusion Complex Preparation Methods: Effect of Common Solvent Evaporation, Freeze Drying, and pH Shift on Solubility and Stability of Curcumin

Jantarat

Sirathanarun²,

Ratanapongsai³

et al. 2014

Trop. J. Pharm Res

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Modulatory Effect of Phytoestrogens and Curcumin on Induction of Annexin 1 in Human Peripheral Blood Mononuclear Cells and their Inhibitory Effect on Secretory Phospholipase A<sub>2</sub>

Cited by 7 publications

References 23 publications

Evaluation of phytoestrogens in inducing cell death mediated by decreasing Annexin A1 in Annexin A1-knockdown leukemia cells

Evaluation of phytoestrogens in inducing cell death mediated by decreasing Annexin A1 in Annexin A1-knockdown leukemia cells

Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines

Curcumin-Hydroxypropyl-β-Cyclodextrin Inclusion Complex Preparation Methods: Effect of Common Solvent Evaporation, Freeze Drying, and pH Shift on Solubility and Stability of Curcumin

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Modulatory Effect of Phytoestrogens and Curcumin on Induction of Annexin 1 in Human Peripheral Blood Mononuclear Cells and their Inhibitory Effect on Secretory Phospholipase A<sub>2</sub>

Cited by 7 publications

References 23 publications

Evaluation of phytoestrogens in inducing cell death mediated by decreasing Annexin A1 in Annexin A1-knockdown leukemia cells

Evaluation of phytoestrogens in inducing cell death mediated by decreasing Annexin A1 in Annexin A1-knockdown leukemia cells

Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines

Curcumin-Hydroxypropyl-&#946;-Cyclodextrin Inclusion Complex Preparation Methods: Effect of Common Solvent Evaporation, Freeze Drying, and pH Shift on Solubility and Stability of Curcumin

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Curcumin-Hydroxypropyl-β-Cyclodextrin Inclusion Complex Preparation Methods: Effect of Common Solvent Evaporation, Freeze Drying, and pH Shift on Solubility and Stability of Curcumin