Modulatory effect of phytoglycoprotein (38 kDa) on cyclin D1/CDK4 in BNL CL.2 cells induced by N-methyl-N′-nitro-N-nitrosoguanidine

Liu, Jin; Lim, Kye-Taek

doi:10.1007/s00210-011-0699-6

Cited by 2 publications

(1 citation statement)

References 25 publications

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“…For example, norlignan and styraxlignolide have anti-complement activity [ 7 ] and styraxoside A has anti-inflammatory effects in cultured cells [ 8 ]. In addition, phytoglycoprotein (38 kDa) from SJSZ suppresses cell proliferation in human carcinomatous cells [ 9 , 10 ] and mice [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Toxicity of Jegosaponins A and B from Styrax japonica Siebold et al. Zuccarini in Prostate Cancer Cells and Zebrafish Embryos Resulting from Increased Membrane Permeability

Nishimura

Fuchino

Takayanagi

et al. 2021

IJMS

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(1) Background: Screening of medicinal herbs is one of the most powerful approaches to identifying novel therapeutic molecules against many human diseases. To avoid potential harmful effects during medicinal use, toxicity testing is necessary in the early stages of drug discovery. The objective of this study was to identify the cytotoxic mechanisms of jegosaponin A and B from Styrax japonica Siebold et al. Zuccarini; (2) Methods: We screened Japanese medicinal herb extracts using PC-3 prostate cancer cells and found that a methanol extract isolated from the unripe fruit of Styrax japonica Siebold et al. Zuccarini (SJSZ) had an inhibitory effect on cell viability. We further performed fractionation assays with PC-3 cells and identified the bioactive compounds using LC/MS and NMR analysis. We clarified the toxic mechanisms of these compounds using PC-3 cells and zebrafish embryos; (3) Results: We identified two active molecules, jegosaponin A and jegosaponin B, in the inhibitory fractions of the methanol extract. These jegosaponins are toxic to zebrafish embryos during the early developmental stage. Jegosaponin A and B showed strong haemolytic activity in sheep defibrinated blood (EC50 = 2.1 μM, and 20.2 μM, respectively) and increased the cell membrane permeability in PC-3 cells and zebrafish embryos, which were identified using a membrane non-permeable DRAQ7, a fluorescent nucleus staining dye; (4) We identified the cytotoxic compounds jegosaponin A and B from SJSZ, which we showed to exhibit cell membrane disruptive properties using cell- and zebrafish-based testing.

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Section: Introductionmentioning

confidence: 99%

Toxicity of Jegosaponins A and B from Styrax japonica Siebold et al. Zuccarini in Prostate Cancer Cells and Zebrafish Embryos Resulting from Increased Membrane Permeability

Nishimura

Fuchino

Takayanagi

et al. 2021

IJMS

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Phytoglycoproteins and Human Health: Current Knowledge and Future Applications

et al. 2021

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Over the years, humans have relied on plants as sources of nutrients and bioactive compounds that promote health and wellness. Interestingly, drug discovery has benefitted immensely from the use of bioactive phytochemicals derived from food and medicinal plants. Phytoglycoproteins (PGPs) are plant-derived proteins with sugar moieties covalently linked to amino acid side chains, formed by glycosylation during posttranslational modification of polypeptides. Several studies in the last two decades, including cell culture and animal studies, have documented a variety of health-beneficial effects of PGPs, including hypolipidemic, wound healing, antioxidant, anti-inflammatory, immunomodulatory, and anticancer properties. Despite the prospects, there is a dearth of information on the pharmacokinetics and toxicity of PGPs, including possible induction of immune reactions, and the potential effects of stereospecific variation in PGPs isomers on their physiological functions. Further exploration of the multifunctional glycoproteins will position them as strong candidates for the development of nutraceuticals and functional foods.

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Modulatory effect of phytoglycoprotein (38 kDa) on cyclin D1/CDK4 in BNL CL.2 cells induced by N-methyl-N′-nitro-N-nitrosoguanidine

Cited by 2 publications

References 25 publications

Toxicity of Jegosaponins A and B from Styrax japonica Siebold et al. Zuccarini in Prostate Cancer Cells and Zebrafish Embryos Resulting from Increased Membrane Permeability

Toxicity of Jegosaponins A and B from Styrax japonica Siebold et al. Zuccarini in Prostate Cancer Cells and Zebrafish Embryos Resulting from Increased Membrane Permeability

Phytoglycoproteins and Human Health: Current Knowledge and Future Applications

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