Modulatory effects of caffeine on methotrexate-induced cytogenotoxicity in mouse bone marrow

Choudhury, Ramesh C.; Palo, Anil K.

doi:10.1016/j.etap.2003.10.001

Cited by 24 publications

(11 citation statements)

References 30 publications

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“…However, is worth mentioning that some of the components present in soft drinks are potentially harmful to the human body as a whole, in particular to the genetic material (ANTUNES; ARAÚJO, 2000;CHOUDHURY;PALO, 2004;KEAST;RIDDELL, 2007;RAYES, 2008;COLUCCI et al, 2011). Thus, considering that few studies have been conducted to evaluate the cytotoxic and mutagenic potential of soft drinks, the aim of this study was to investigate this potential of specific cola and grape-flavored soft drink brands in Wistar rats.…”

Section: Resultsmentioning

confidence: 99%

Cytotoxicity and mutagenicity of cola and grape flavored soft drinks in bone marrow cells of rodents

et al. 2013

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Due to the large consumption of soft drinks in Brazil and worldwide in recent years and considering that some of the components present in their composition pose potential risks to human health, the aim of this study was to evaluate the cytotoxic and mutagenic potential of specific cola and grape-flavored soft drink brands. Bone marrow cells of Wistar rats were initially treated by gavage with one single dose of Cola or Grape soft drink, which was next offered ad libitum (instead of water) for 24 hours. A negative control treatment was performed by administering one single dose of water and a positive control administering cyclophosphamide intraperitoneally. Statistical analysis showed that the Cola and Grape soft drinks studied were not cytotoxic. However, the Cola soft drink proved mutagenic in this experiment treatment time. Therefore, this study serves as a warning about the consumption of Cola-flavored soft drink and for the need for further subchronic and chronic studies on soft drinks in order to evaluate the long term mutagenic and cytotoxic effects of these substances.

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Section: Resultsmentioning

confidence: 99%

Cytotoxicity and mutagenicity of cola and grape flavored soft drinks in bone marrow cells of rodents

et al. 2013

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“…The genotoxic effect of MXT have been observed in both somatic and germ cells (Choudhury and Palo, 2004). In addition, there were several studies evaluated the effect of MXT on fertility.…”

Section: Introductionmentioning

confidence: 99%

Effect of Grape Seed Extract, Gervital, Against Methotrexate Induced Histological and Ultrastructural Alterations in Testes of Albino Rats

Arisha¹

2017

WJPR

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The present study was designed to investigate the effect of Gervital on testicular damage induced by the anticancer drug, methotrexate (MXT), in adult male albino rats. Forty male albino rats (Rattus norvegicus) were divided into five experimental groups (8 animals in each one) Group 1: served as control group. Group 2: animals of this group were orally administered grape seed extract only at a dose level of 150mg/kg body weight daily for 28 days. Group 3: animals of this group were intraperitoneally injected with a single dose of MXT (8mg/kg body weight) per week for 4 weeks. Group 4: animals of this group were intraperitoneally injected with a single dose of MXT followed by grape seed extract (GSE) daily for 28 days. Group 5: animals of this group were pretreated with GSE followed by MXT. At the end of the experimental period, all rats were sacrificed. Blood samples were collected for the biochemical study and testes were removed and prepared for histological and ultrastructural studies. Results: Methotrexate significantly reduced the final body and testes weight. Histological observations: several pathological changes were observed in the testicular tissues. These changes include degenerated seminiferous tubules, distorted spermatogenic cells and degenerated interstitial tissue with hemorrhage.Ultrastructural examination showed various severe degenerated features after MXT treatment including types A&B spermatogonia, round and elongated spermatids. On the other hand, GSE administration showed advanced degree of improvement in all the histopathological and ultrastructural changes; the testicular tissues appeared mostly normal.Biochemical analysis revealed that MXT injection significantly reduced serum testosterone level and superoxide dismutase (SOD) activity while, it significantly elevated malondialdehyde (MDA) level. Otherwise, administration of gervital restored the previous

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“…Drugs with at least one result in in vivo tests for SCE, chromosomal aberrations, or micronucleus in animal or human cells (2,3,5,6,7,8,9,10,11,12,13,14,17,18,19,20,21) 17 (37.0%)…”

Section: Epidemiological Studiesmentioning

confidence: 99%

“…Drugs that underwent testing in other types of genotoxicity assays (3,5,6,7,9,10,13,15,17,18) 10 (21.7%)…”

Section: Epidemiological Studiesmentioning

confidence: 99%

“…According to Tata et al [16], there is some evidence of a modest increase in congenital malformations in children born to women with asthma, but this was not explained by gestational exposure to asthma drugs. No data are available [14] to evaluate the carcinogenicity to human Drugs with at least one result in tests for gene mutation in mammalian cells (3,5,8,9,10,11,14,17,18,19,20) 11 (23.9%) Drugs with at least one result in in vitro tests for SCE, chromosomal aberrations, aneuploidy or micronucleus in animal or human cells (1,3,4,5,7,8,10,11,13,14,15,17,18,19,20,21) 16 (34.8%)…”

Section: Epidemiological Studiesmentioning

confidence: 99%

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Genotoxicity and Carcinogenicity Studies of Bronchodilators and AntiAsthma Drugs

Brambilla

Mattioli

Robbiano

et al. 2013

Basic Clin Pharma Tox

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This survey is a compendium of genotoxicity and carcinogenicity information of bronchodilators and antiasthma drugs. Data from 46 marketed drugs were collected. Of these 46 drugs, 25 (54.3%) did not have retrievable genotoxicity or carcinogenicity data. The remaining 21 (45.7%) had at least one genotoxicity or carcinogenicity test result. Of these 21 drugs, 10 had at least one positive finding: three tested positive in at least one genotoxicity assay, eight in at least one carcinogenicity assay, and one of them gave positive results in both genotoxicity assay and carcinogenicity assay. Concerning the predictivity of genetic toxicology findings for the result(s) of long-term carcinogenesis assays, 15 drugs had both genotoxicity and carcinogenicity data: seven of them (46.6%) were neither genotoxic nor carcinogenic, 6 (40.0%) were carcinogenic in at least one sex of mice or rats but tested negative in genotoxicity assays, 1 (6.7%) tested positive in genotoxicity assay but was non-carcinogenic, and 1 (6.7%) gave positive responses in both genotoxicity and carcinogenicity assay. Only 11 (23.9%) of the 46 drugs considered had all data required by current guidelines for testing of pharmaceuticals, but a large fraction of them were developed and marketed prior to the present regulatory climate.Bronchodilators and antiasthma drugs are continuously or frequently used in the treatment for chronic airways diseases. Among the various adverse reactions of concern that these pharmaceuticals might cause, genotoxic and carcinogenic effects cannot be excluded. Therefore, we deemed useful to verify to what extent the drugs of this family have been tested for their potential genotoxic and carcinogenic risk to human beings.The regulatory authorities of USA, Europe and Japan recommend th at genotoxicity and carcinogenicity assays are performed before application for marketing approval of pharmaceuticals. Present guidelines for genotoxicity testing of pharmaceuticals [1-3] indicate a standard test battery that consists of (i) a test for gene mutation in bacteria; (ii) an in vitro test with cytogenetic evaluation of chromosomal damage with mammalian cells or an in vitro mammalian cells gene mutation assay and (iii) an in vivo test for chromosomal damage performed with rodent hematopoietic cells. Guidelines for carcinogenicity testing of pharmaceuticals [4,5] indicate that long-term carcinogenicity studies in rodents should be performed for all pharmaceuticals whose expected clinical use is continuous for at least 6 months as well as for pharmaceuticals used frequently in an intermittent manner in the treatment for chronic recurrent conditions. In long-term carcinogenicity assays, the highest dose should be at least 25 times higher, on a mg/m 2 basis, than the maximum recommended human daily dose or represent a 25-fold ratio of rodent to human AUC. In the chapter bronchodilators and antiasthma drugs of the 2007 edition of Martindale: The Complete Drug Reference [6] are included 46 drugs still on the market, the majority of wh...

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Modulatory effects of caffeine on methotrexate-induced cytogenotoxicity in mouse bone marrow

Cited by 24 publications

References 30 publications

Cytotoxicity and mutagenicity of cola and grape flavored soft drinks in bone marrow cells of rodents

Cytotoxicity and mutagenicity of cola and grape flavored soft drinks in bone marrow cells of rodents

Effect of Grape Seed Extract, Gervital, Against Methotrexate Induced Histological and Ultrastructural Alterations in Testes of Albino Rats

Genotoxicity and Carcinogenicity Studies of Bronchodilators and AntiAsthma Drugs

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