Modulatory Mechanism of Polyphenols and Nrf2 Signaling Pathway in LPS Challenged Pregnancy Disorders

Hussain, Tarique; Tan, Bie; Liu, Gang; Murtaza, Ghulam; Rahu, Najma; Saleem, Muhammad; Yin, Yulong

doi:10.1155/2017/8254289

Cited by 34 publications

(20 citation statements)

References 134 publications

(133 reference statements)

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“…Subsequently, the relationship between mi-34a and LGR4 was identi ed by the dual-luciferase reporter assay, which showed that miR-34a drastically lowered the activity of LGR4 3'UTR. Similarly, it has been recently reported that LGR4 is a common target of miR-34a and miR-34c in mice [30]. In conjunction with the above study results, that LGR4 inhibits the production of pro-in ammatory mediators, subsequently, we transfected miR-34a mimic or inhibitor to BENDs to carry out function gain and function loss experiments in order to further investigate whether there was a suppression effect of miR-34a on LGR4.…”

Section: Discussionsupporting

confidence: 70%

IL-1β mediates miR-34a promotes bovine endometritis via LGR4-NF-κB axis

Yin

Guo

et al. 2021

Preprint

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Background Persistent endometritis lead by bacterial infections has lethal effects on the reproductive performance of dairy cattle, which not only compromise animal welfare but also delay or prevent pregnancy. MicroRNA (miRNA) miR-34 family plays a pivotal role in the inflammatory process; however, the precise mechanism of miR-34a in endometritis is still not thoroughly revealed. Methods In this study, we established bovine endometrial epithelial cell (BENDs) inflammation model and mouse model stimulation with Lipopolysaccharide (LPS) in vitro and in vivo. CCK-8 was used to assess cell viability. H&E was used to characterize morphology. Immunohistochemistry, immunofluorescence, qRT-PCR and western blot assays were performed to measure the mRNA or protein expression of related genes. Online database, plasmid construction and dual-luciferase Reporter gene assays were applied to predict and validate the interaction between miR-34a and its target gene LGR4, and mice were injected vaginally with local antagomir to validate the role of miR-34a in murine uterine inflammation. Results Here, we report that miR-34a suppresses LGR4 gene expression by targeting its 3'untranslated regions (3'UTR). The miR-34a was up-regulated in cow uterine tissues and bovine endometrial epithelial cell (BENDs) stimulation with LPS. It further induces the release of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α by activating the phosphorylation level of NF-κB p65. Furthermore, we also revealed that IL-1β was responsible for the upregulation of miR-34a transcription and downregulation of LGR4 in an IL-1β-dependent manner. Conclusions Taken together, our study confirmed that miR-34a is regulated by IL-1β and suppress the level of LGR4 3’UTR which in turn exacerbates the inflammatory response. Thus knockdown miR-34a might be a new indirection for treatment endometritis.

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Section: Discussionsupporting

confidence: 70%

IL-1β mediates miR-34a promotes bovine endometritis via LGR4-NF-κB axis

Yin

Guo

et al. 2021

Preprint

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“…In addition, probiotics have positive impacts on colonized beneficial bacterial and growth performance in broilers and pigs (Jeong and Kim, 2014; Valeriano et al, 2017). Noticeably, probiotics, as the alternatives for antibiotics used to prevent poultry diseases and improve production performance, have been demonstrated to be beneficial to chickens’ growth performance and health, such as the increases of body weight (BW), feed conversion efficiency, immune response, resistance to bacterial infection, and regulation of intestinal microflora (Xu et al, 2012; Cao et al, 2013; Song et al, 2014; Clavijo and Flórez, 2017; Haque et al, 2017; Hussain et al, 2017a; Azad et al, 2018; Mashayekhi et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Intestinal Morphologic and Microbiota Responses to Dietary Bacillus spp. in a Broiler Chicken Model

Wang

Zhang

et al. 2019

Front. Physiol.

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Dietary inclusion of probiotic Bacillus spp. beneficially affect the broiler chickens by balancing the properties of the indigenous microbiota causing better growth performance. The effects of three Bacillus spp. on the growth performance, intestinal morphology and the compositions of jejunal microflora were investigated in broiler chickens. A total of 480 1-day-old male Arbor Acres broilers were randomly divided into four groups. All groups had six replicates and 20 birds were included in each replicate. The control birds were fed with a corn-soybean basal diet, while three treatment diets were supplemented with Bacillus coagulans TBC169, B. subtilis PB6, and B. subtilis DSM32315 with a dosage of 1 × 109 cfu/kg, respectively. The experiment lasted for 42 days. The compositions and diversity of jejunal microflora were analyzed by MiSeq high-throughput sequencing. The B. coagulans TBC169 group showed marked improvements of growth performance, nutrient digestibility and intestinal morphology compared with the other B. subtilis treatments. B. coagulans TBC169 supplementation improved the average body weight (BW), average daily weight gain (ADG), total tract apparent digestibility of crude protein and gross energy (GE), and reduced feed conversion rate (FCR) compared with the control group (P < 0.05). The villus height to crypt depth ratio (VH/CD) of jejunum and duodenum was increased in the birds fed with B. coagulans TBC169 compared with the control group (P < 0.05). However, two B. subtilis treatments presented more positive variation of the jejunum microflora of chickens than that in the B. coagulans TBC169 group. B. subtilis PB6 and B. subtilis DSM32315 treatments improved the diversity of jejunal microbiota on day 21 compared with the control (P < 0.05), while which were decreased on day 42 (P < 0.05). The supplementation with B. coagulans TBC169 significantly improved the proportion of Firmicutes, otherwise two B. subtilis significantly improved the proportion of Proteobacteria, Bacteroidetes, Actinobacteria, and Acidobacteria at the phylum level during starter phase and decreased the proportion of Bacteroidetes during growing phase compared with the control. The supplementation with B.subtilis DSM32315 significantly improved the proportion of Clostridiales during starter phase, whereas two B. subtilis significantly improved the proportion of Pseudomonas, Burkholderia, Prevotella, DA101 during growing phase at the genus level compared with the control. In conclusion, the dietary supplementation with probiotic Bacillus spp. strains improved body weight and intestinal morphology in broiler chickens, which might be associated with the gut microbiota.

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“…The maximum absorption peaks of p -hydroxybenzoic acid or hydroxycinnamic acid are detected at 280 and 320 nm, respectively [ 17 , 18 ]. Ferulic acid inhibits the production of tumour necrosis factor- (TNF-) α in RAW264.7 cells stimulated with lipopolysaccharide (LPS) [ 19 , 20 ]. Immunological studies have shown that phenyl ethyl caffeate strongly and significantly inhibits the expression of interferon gamma-induced protein- (IP-) 10 in response to TNF, as well as the production of lymphoid factors and activation of nuclear factor- (NF-) κ B [ 21 – 23 ].…”

Section: The Structure and Function Of Polyphenolsmentioning

confidence: 99%

Regulation of Immune Function by Polyphenols

Ding

Jiang

Fang

2018

Journal of Immunology Research

212

143

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Immune dysfunction is caused by various factors, including changes in relevant immune regulators and environmental stress. Immune system imbalance leads to a variety of diseases in humans. Nutrition may play an essential role in immunity by interfering with proinflammatory cytokine synthesis, immune cell regulation, and gene expression. Polyphenols, one of many categories of natural substances, exhibit a range of biological activities. Polyphenols promote immunity to foreign pathogens via various pathways. Different immune cells express multiple types of polyphenol receptors that recognise and allow cellular uptake of polyphenols, which subsequently activate signalling pathways to initiate immune responses. Furthermore, the polyphenols curcumin and epigallocatechin gallate can induce epigenetic changes in cells. In summary, polyphenols can be used to regulate intestinal mucosal immune responses, allergic diseases, and antitumour immunity.

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Modulatory Mechanism of Polyphenols and Nrf2 Signaling Pathway in LPS Challenged Pregnancy Disorders

Cited by 34 publications

References 134 publications

IL-1β mediates miR-34a promotes bovine endometritis via LGR4-NF-κB axis

IL-1β mediates miR-34a promotes bovine endometritis via LGR4-NF-κB axis

Intestinal Morphologic and Microbiota Responses to Dietary Bacillus spp. in a Broiler Chicken Model

Regulation of Immune Function by Polyphenols

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