MOF‐Based Nanotubes to Hollow Nanospheres through Protein‐Induced Soft‐Templating Pathways

Abstract

A controllable and facile strategy is established for constructing metal–organic frameworks‐based (MOF‐based) hollow composites via a protein‐induced soft‐templating pathway. Using metal‐sodium deoxycholate hydrogel as soft‐template, nanotubes are gained while the protein is absent. With the presence of protein, hollow nanospheres structure are prepared by changing the amount of protein. To verify the universality of the proposed pathway, two kinds of proteins ( Burkholderia cepacia lipase and penicillin G acylase) and three kinds of MOF (ZIF‐8, ZIF‐67, and Fe‐MOF) are adopted as model proteins and materials, and the obtained protein‐containing composites (named protein@H‐MOF) possess high bioactivity and stability. This proposed strategy provides a facile method for preparing MOF‐based composites under mild conditions, facilitating the applications of MOF in the fields of biocatalyst construction, biomolecule encapsulation, and drug delivery.

Enzyme Encapsulation in a Porous Hydrogen-Bonded Organic Framework

“…Zeolitic imidazolate framework-8 (ZIF-8), and related ZIF materials, have been proven to be excellent candidates for the synthesis of bio-composites. [15][16][17][18] Although ZIFs possess many properties that make them favorable for studying biomolecule encapsulation, they present challenges for certain applications. For example, the structure topologies observed for reported ZIF biocomposites possess narrow crystallographic pore apertures (typically ~ 3.4 Å) that significantly limit the size of substrates that can be used for biocatalytic transformations.…”

Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors

“…Moreover, the immobilization amount of RuBisCO is still small due to the limited space of the microreactor. Some new material such as MOFs 59 , COFs 60 , mesoporous nanoparticles 61 , and carbon nitride 62 could be applied to the microreactor to provide larger surface areas in the future. The reactor can also be easily scaled up 63–66 to increase the output and more functionalities can be integrated, such as deoxygenation, temperature control of individual reaction, etc.…”

Biocatalytic Metal–Organic Frameworks: Prospects Beyond Bioprotective Porous Matrices

“…[71][72][73][74] To this end, Jiang and co-workers recently developed ZIF-8-based hollow nanoparticles by a protein-induced soft-templating synthetic strategy (Figure 3c, i). [75] Hollow [75] Another emulsification templating strategy was developed by Tan and co-workers (Figure 3c, ii). [76] Using hydrogel as a template, zinc-based hydrogels was obtained through H-bonds formation between salicylic acid and melamine, where Zn 2+ are uniformly distributed.…”

“…Protein-induced softtemplating Hollow BCL@H-ZIF-8 exhibited 69.58% activity recovery. [75] Increasing the pore size A templated emulsification method Enzyme/HZIF-8 shows 2.7 times and 7.7 times higher enzymatic activity. [76] Hierarchical Zr-MOF Larger pore sizes lead to faster reaction kinetics.…”