Mogroside V protects porcine oocytes from in vitro ageing by reducing oxidative stress through SIRT1 upregulation

Nie, Junyu; Sui, Lumin; Zhang, Huiting; Zhang, Hengye; Yan, Ke; Yang, Xiaogan; Lu, Sen; Ke-huan, LU; Liang, Xingwei

doi:10.18632/aging.102324

Cited by 35 publications

(29 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Then, we applied the porcine oocyte as a study model and found that MV treatment evidently alleviated the LPS-induced meiotic defects. Similarly, we previously revealed that MV has protective and promotive effects on porcine oocyte maturation and competence ( Nie et al, 2019 , 2020 ). Thus, our findings suggest that MV is a candidate agent for amelioration of LPS-induced oocyte meiotic defects.…”

Section: Discussionsupporting

confidence: 55%

“…As a potent antioxidant, MV has significant oxygen free radical scavenging capacity and attenuates lipid peroxidation induced by Fe 2+ or H 2 O 2 ( Li et al, 2014 ). In addition, our previous studies demonstrated that MV reduces reactive oxygen species (ROS) levels and promotes mitochondrial function to improve porcine oocyte IVM ( Nie et al, 2020 ) and protects oocyte quality from deterioration during in vitro aging ( Nie et al, 2019 ). Furthermore, protective effects of MV against LPS-induced diseases, such as alleviation of LPS-induced memory impairment and acute lung injury in mice and prevention of LPS-induced oxidative stress injury in RAW264.7 cells have been reported ( Di et al, 2011 ; Shi et al, 2014 ; Li et al, 2019 ; Wang et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mogroside V Protects Porcine Oocytes From Lipopolysaccharide-Induced Meiotic Defects

Yan

Cui

Nie

et al. 2021

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

Accumulating evidence has demonstrated that lipopolysaccharide (LPS) compromises female reproduction, especially oocyte maturation and competence. However, methods to protect oocyte quality from LPS-induced deterioration remain largely unexplored. We previously found that mogroside V (MV) can promote oocyte maturation and embryonic development. However, whether MV can alleviate the adverse effects of LPS exposure on oocyte maturation is unclear. Thus, in this study, we used porcine oocytes as a model to explore the effects of MV administration on LPS-induced oocyte meiotic defects. Our findings show that supplementation with MV protected oocytes from the LPS-mediated reduction in the meiotic maturation rate and the subsequent blastocyst formation rate. In addition, MV alleviated the abnormalities in spindle formation and chromosome alignment, decrease in α-tubulin acetylation levels, the disruption of actin polymerization, and the reductions in mitochondrial contents and lipid droplet contents caused by LPS exposure. Meanwhile, LPS reduced m6A levels in oocytes, but MV restored these epigenetic modifications. Furthermore, MV reduced reactive oxygen species (ROS) levels and early apoptosis in oocytes exposed to LPS. In summary, our study demonstrates that MV can protect oocytes from LPS-induced meiotic defects in part by reducing oxidative stress and maintaining m6A levels.

show abstract

Section: Discussionsupporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Mogroside V Protects Porcine Oocytes From Lipopolysaccharide-Induced Meiotic Defects

Yan

Cui

Nie

et al. 2021

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Still oocyte aging in vitro results in the abnormal distribution and function disorder of cytoplasmic organelles. For example, the most widely studied organelle is mitochondria, with confirmed dysfunction in aged oocytes such as low membrane potential, reduced ATP content and mutated mitochondrial DNA 57 , 58 . The aged porcine oocytes has been reported to exhibit disordered ER clusters and impaired ER calcium homeostasis 59 .…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effects of astaxanthin on postovulatory porcine oocyte aging in vitro

Jia

Xiang

Shao

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Mammalian oocytes represent impaired quality after undergoing a process of postovulatory aging, which can be alleviated through various effective ways such as reagent treatment. Accumulating evidences have revealed the beneficial effects of astaxanthin (Ax) as a potential antioxidant on reproductive biology. Here, porcine matured oocytes were used as a model to explore whether Ax supplement can protect against oocyte aging in vitro and the underlying mechanism, and therefore they were cultured with or without 2.5 μM Ax for an additional 24 h. Aged oocytes treated with Ax showed improved yield and quality of blastocysts as well as recovered expression of maternal genes. Importantly, oxidative stress in aged oocytes was relieved through Ax treatment, based on reduced reactive oxygen species and enhanced glutathione and antioxidant gene expression. Moreover, inhibition in apoptosis and autophagy of aged oocyte by Ax was confirmed through decreased caspase-3, cathepsin B and autophagic activities. Ax could also maintain spindle organization and actin expression, and rescue functional status of organelles including mitochondria, endoplasmic reticulum, Golgi apparatus and lysosomes according to restored fluorescence intensity. In conclusion, Ax might provide an alternative for ameliorating the oocyte quality following aging in vitro, through the mechanisms mediated by its antioxidant properties.

show abstract

“…Furthermore, MV reduced intracellular ROS and increased the mRNA expression of oxidative stress-related genes (SOD, CAT, and SIRT1) while enhancing mitochondrial function ( Nie et al, 2020 ). MV markedly reduces the decline in porcine oocyte quality during in vitro aging, possibly by reducing oxidative stress and early apoptosis in aged oocytes while improving mitochondrial contents and function ( Nie et al, 2019 ).…”

Section: Natural Antioxidant-based Interventions To Reduce Oxidative mentioning

confidence: 99%

The Role of Oxidative Stress and Natural Antioxidants in Ovarian Aging

et al. 2021

View full text Add to dashboard Cite

The ovarian system comprises vital organs in females and is of great significance for the maintenance of reproductive potential and endocrine stability. Although complex pathogenesis undoubtedly contributes to ovarian aging, increasing attention is being paid to the extensive influence of oxidative stress. However, the role of oxidative stress in ovarian aging is yet to be fully elucidated. Exploring oxidative stress-related processes might be a promising strategy against ovarian aging. In this review, compelling evidence is shown that oxidative stress plays a role in the etiology of ovarian aging and promotes the development of other ovarian aging-related etiologies, including telomere shortening, mitochondrial dysfunction, apoptosis, and inflammation. In addition, some natural antioxidants such as quercetin, resveratrol, and curcumin have a protective role in the ovaries through multiple mechanisms. These findings raise the prospect of oxidative stress modulator-natural antioxidants as therapeutic interventions for delaying ovarian aging.

show abstract

Mogroside V protects porcine oocytes from in vitro ageing by reducing oxidative stress through SIRT1 upregulation

Cited by 35 publications

References 40 publications

Mogroside V Protects Porcine Oocytes From Lipopolysaccharide-Induced Meiotic Defects

Mogroside V Protects Porcine Oocytes From Lipopolysaccharide-Induced Meiotic Defects

Inhibitory effects of astaxanthin on postovulatory porcine oocyte aging in vitro

The Role of Oxidative Stress and Natural Antioxidants in Ovarian Aging

Contact Info

Product

Resources

About