Molds and mycotoxins in poultry feeds from farms of potential mycotoxicosis

Shareef, A. M.

doi:10.33899/ijvs.2010.5581

Cited by 38 publications

(40 citation statements)

References 21 publications

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“…Mucor, Aspergillus, Fusarium and Penicillium were recovered as predominate genera from poultry feed by Shareef (2010) somewhat related to present study. Labuda and Tančinová (2006) reported the isolation of Penicillium (89%), Aspergillus (69%), Mucor (50%) and Cladosporium (31%) from feed samples.…”

Section: Discussionsupporting

confidence: 81%

“…Moulds (filamentous fungi) not only affect the organoleptic properties of feed but some are toxin producers (Greco et al, 2014). In present study, mould counts in home mixed (1.64 x 10 4 ) and commercial feed (6.0 x 10 3 CFU/g) were slightly lower than counts in poultry feed (6.5x10 6 CFU/g) in Iraq (Shareef, 2010), counts (42 × 10 3 CFU/g) in Nigarian poultry feed (Kehinde et al, 2014), fungal load (0-14 x 10 4 CFU/g) in Serbia's poultry feed (Krnjaja et al, 2014), counts of fungi (8.1 x 10 5 CFU/g) in Nigarian bird's feed (Matthew et al, 2017) and higher than fungal counts (3x10 2 -4x10 4 CFU/g) in poultry feed in Quetta, Pakistan (Rashid et al, 2017) and in Iran; mean fugal count 6.4×10 4 ± 1.12×10 5 CFU/g of poultry feed (Parviz et al, 2014). No doubt all poultry feed samples (n=40) of present study were categorized as good (Gimeno et al, 2002) in contrast to above mentioned studies.…”

Section: Discussioncontrasting

confidence: 50%

“…Above mentioned data showed more diversity in fungal isolates present in poultry feed opposite to present findings. The differences or similarities in diversity might be due to climatic conditions prevailing in different regions (Shareef, 2010). But all studies strengthen the presence of opportunistic fungal species in poultry feed.…”

Section: Discussionmentioning

confidence: 91%

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Molecular Approaches for Characterization of Aflatoxin Producing Aspergillus flavus Isolates from Poultry Feed

Sana¹

2019

PVJ

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Aflatoxins are secondary toxic metabolites produced by aspergilli. Aspergillus flavus is one of the major aflatoxins producing specie. Present study was conducted to enumerate mycoflora of poultry feed and aflatoxin production potential of A. flavus. Home mixed and commercial poultry feed (n=20, each) were processed for determination of fungal load and isolation of mycoflora. Isolates were identified by culture and microscopic characters. Thin layer (TL) and high-performance liquid chromatography (HPLC) were used for screening, identification and quantification of aflatoxins produced by A. flavus respectively. A. flavus were confirmed by specie specific polymerase chain reaction (PCR). Isolation frequency of different genera, Aspergillus species and toxigenic A. flavus was calculated. The fungal count in home mixed feed was 2x10 2 to 1.6x10 4 CFU/g whereas, in commercial poultry feed from 2x10 1 to 6x10 3 CFU/g. Aspergillus was the most prevalent genus in home mixed and commercial feed followed by Mucor. Among aspergilli, the highest percentage was of flavus (95%) followed by A. niger (75%), A. fumigatus (15%) and A. terreus (5%). A total of 32.61 percent (223/685) aflatoxin producing A. flavus from commercial and 16.67 percent (23/140) from home mixed feed were detected by TLC. These aflatoxins (AFs) were identified as AFB1 and AFB2 and AFG1 by HPLC. Amplicon (500 bps) of A. flavus was observed on 2 percent agarose gel. It was concluded that poultry feed may be a source of transmission of disease producing fungi and aflatoxins to poultry birds and human beings. ©2019 PVJ. All rights reserved Key words: Aflatoxin Aspergillus flavus commercial poultry feed High performance liquid chromatography Home mixed poultry feed Polymerase chain reaction To Cite This Article: Sana S, Anjum AA, Yaqub T, Nasir M, Ali MA and Abbas M, 2019. Molecular approaches for characterization of aflatoxin producing Aspergillus flavus isolates from poultry feed. Pak Vet J, 39(2): 169-174. http://dx.

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Section: Discussionsupporting

confidence: 81%

Section: Discussioncontrasting

confidence: 50%

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Molecular Approaches for Characterization of Aflatoxin Producing Aspergillus flavus Isolates from Poultry Feed

Sana¹

2019

PVJ

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“…The combined action of mycotoxins can generate interactive pathological and toxicological effects. Our results agreed with Mariana et al [34] ; Shareef [35] ; Abdel Gabbar and Saad [36] ; Charlton [37] ; Rajendra et al [38] and were in partial agreement with Adel et al [39] . The negative effects of mycotoxins on chicken performance have been demonstrated.…”

Section: Study Of Mycotoxins In Poultry Feeds Samples-feedsupporting

confidence: 93%

Toxicological and Pathological Effects of Mycotoxins in Poultry in Saudi Arabia

Hassan¹,

Barri²,

Mohammed³

et al. 2019

SSR-IIJLS

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Background: Mycotoxins are secondary metabolites of low molecular weight produced by a wide range of fungi, principally molds. There are over 200 species of molds that produced mycotoxins of fungi. This study was carried out in Al-Qassim region-Saudi Arabia to investigate the occurrence of mycotoxins in poultry feed to identify their different groups and study the toxicological and the pathological effects of these toxins. Methods: Samples of the poultry feeds were collected from different poultry farms and were analyzed by quantitative using ELISA. A portion of the tissues were collected for histopathology. Results: Mycotoxins were reported to be ubiquitously present as 67.86, 50, 50, 35.71 and 32.14% of the analyzed samples, which reported positive for aflatoxins, ochratoxins, fumonisin, T-2 toxin, and zearalenone, respectively. Representative tissue sections showed necrosis of hepatocytes, glomerular, tubule-interstitial nephritis and necrotic myocarditis. The results indicate that most of these samples were below the maximum regulatory limit. Conclusion: The co-occurrence of several mycotoxins was demonstrated and the combined action of mycotoxins can generate an interactive effect such as additive, synergism, or antagonism.

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“…These reports demonstrated that fungi and mycotoxins were usually present in chinchilla feed, with fungal contamination exceeding the limits (up to 4.5 × 10 4 CFU g −1 ) and the co-occurrence of the five most important mycotoxigenic mould genera recovered at high concentrations. Exposure to these toxic substances may lead to impairment of immune function resulting in increased susceptibility to infectious agents (Shareef 2010). Though the synergic effects of mycotoxins on health and productivity of other animal species such as poultry have been well documented, more studies are needed for chinchillas.…”

Section: Discussionmentioning

confidence: 99%

Fourteen-year status report of fatal illnesses in captive chinchilla (Chinchilla lanigera)

Martino¹,

Bautista²,

Gimeno

et al. 2016

Journal of Applied Animal Research

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Fur ranching has been a prosperous industry for decades. Despite its worldwide distribution, little published information is available regarding the importance of the various causes of death in chinchilla (Chinchilla lanigera). In the period 1999-2013, 698 captive chinchillas from different commercial ranches were presented for necropsy at the Pathology Department (UNLP). Two-hundred and fortyfour animals (35.0%) had classical enteritis, 198 (28.4%) had pneumonia, 63 (9.0%) had other infections, 40 (5.7%) had traumatic injuries, 109 (15.6%) had miscellaneous conditions, meanwhile 44 (6.3%) had no significant lesions. Although some disease processes may be underrepresented (i.e. heat stroke and the shock syndrome), the data were collected from a field wide enough and over a sufficient period to give a reliable overview of the fatal problems of this rodent in captivity. ARTICLE HISTORY

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Molds and mycotoxins in poultry feeds from farms of potential mycotoxicosis

Cited by 38 publications

References 21 publications

Molecular Approaches for Characterization of Aflatoxin Producing Aspergillus flavus Isolates from Poultry Feed

Molecular Approaches for Characterization of Aflatoxin Producing Aspergillus flavus Isolates from Poultry Feed

Toxicological and Pathological Effects of Mycotoxins in Poultry in Saudi Arabia

Fourteen-year status report of fatal illnesses in captive chinchilla (Chinchilla lanigera)

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