Molecular Analysis of Antimicrobial-Susceptible and -Resistant<i>Escherichia coli</i>from Retail Meats and Human Stool and Clinical Specimens in a Rural Community Setting

Hannah, Elizabeth; Johnson, James R.; Angulo, Frederick J.; Haddadin, Bassam; Williamson, Jacquelyn; Samore, Matthew H.

doi:10.1089/fpd.2008.0176

Cited by 35 publications

(35 citation statements)

References 29 publications

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“…Although transmission routes were not investigated food may have been a possible source, as an epidemiological study has previously suggested that frequent pork and chicken consumption were related to multidrug-resistant UTI [25]. Similarity between animal, food, and human ExPEC isolates with respect to phenotypic antimicrobial resistance and virulence genotypes, and other molecular characteristics have been demonstrated by several researchers, including us, supporting the hypothesis that UTI may be a zoonosis [12,13,[26][27][28][29][30][31]. This was substantiated recently by the first study demonstrating virulence of E. coli isolates from healthy production animals and fresh retail meat in a mouse model of ascending UTI [19].…”

Section: Discussionmentioning

confidence: 52%

Is Escherichia coli urinary tract infection a zoonosis? Proof of direct link with production animals and meat

Jakobsen

Garneau

Bruant

et al. 2011

Eur J Clin Microbiol Infect Dis

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Recently, it has been suggested that the Escherichia coli causing urinary tract infection (UTI) may come from meat and animals. The purpose was to investigate if a clonal link existed between E. coli from animals, meat and UTI patients. Twenty-two geographically and temporally matched B2 E. coli from UTI patients, community-dwelling humans, broiler chicken meat, pork, and broiler chicken, previously identified to exhibit eight virulence genotypes by microarray-detection of approximately 300 genes, were investigated for clonal relatedness by PFGE. Nine isolates were selected and tested for in vivo virulence in the mouse model of ascending UTI. UTI and community-dwelling human strains were closely clonally related to meat strains. Several human derived strains were also clonally interrelated. All nine isolates regardless of origin were virulent in the UTI model with positive urine, bladder and kidney cultures. Further, isolates with the same gene profile also yielded similar bacterial counts in urine, bladder and kidneys. This study showed a clonal link between E. coli from meat and humans, providing solid evidence that UTI is zoonosis. The close relationship between community-dwelling human and UTI isolates may indicate a point source spread, e.g. through contaminated meat.

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Section: Discussionmentioning

confidence: 52%

Is Escherichia coli urinary tract infection a zoonosis? Proof of direct link with production animals and meat

Jakobsen

Garneau

Bruant

et al. 2011

Eur J Clin Microbiol Infect Dis

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“…Both phylogenetic groups are typically associated with human ExPEC infections (13,24,25). Strains belonging to these clonal groups are also frequently isolated from food-producing and companion animals and from retail meats (10,11,(26)(27)(28)(29)(30)(31)(32)(33)(34)(35) and cause serious infections in otherwise healthy individuals (6,13). Isolates of the enterobacterial repetitive intergenic consensus 2 (ERIC-2) PCR pattern CgA, later characterized as ST69 by MLST, were the most common uropathogenic E. coli genotype isolated from college students with UTI (36,37).…”

Section: Discussionmentioning

confidence: 99%

Clonal Composition and Community Clustering of Drug-Susceptible and -Resistant Escherichia coli Isolates from Bloodstream Infections

Adams‐Sapper

Diep

Perdreau-Remington

et al. 2013

Antimicrob Agents Chemother

107

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b Multidrug-resistant Escherichia coli strains belonging to a single lineage frequently account for a large proportion of extraintestinal E. coli infections in many parts of the world. However, limited information exists on the community prevalence and clonal composition of drug-susceptible E. coli strains. Between July 2007 and September 2010, we analyzed all consecutively collected Gram-negative bacterial isolates from patients with bloodstream infection (BSI) admitted to a public hospital in San Francisco for drug susceptibility and associated drug resistance genes. The E. coli isolates were genotyped for fimH single nucleotide polymorphisms (SNPs) and multilocus sequence types (MLSTs). Among 539 isolates, E. coli accounted for 249 (46%); 74 (30%) of them were susceptible to all tested drugs, and 129 (52%) were multidrug resistant (MDR). Only five MLST genotypes accounted for two-thirds of the E. coli isolates; the most common were ST131 (23%) and ST95 (18%). Forty-seven (92%) of 51 ST131 isolates, as opposed to only 8 (20%) of 40 ST95 isolates, were MDR (P < 0.0001). The Simpson's diversity index for drug-susceptible ST genotypes was 87%, while the index for MDR ST genotypes was 81%. ST95 strains were comprised of four fimH types, and one of these (f-6) accounted for 67% of the 21 susceptible isolates (P < 0.003). A large proportion (>70%) of both MDR and susceptible E. coli BSI isolates represented community-onset infections. These observations show that factors other than the selective pressures of antimicrobial agents used in hospitals contribute to community-onset extraintestinal infections caused by clonal groups of E. coli regardless of their drug resistance.

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“…Our results allow a fundamental step forward in the understanding of the full virulence potential and, to a lesser extent, of the antimicrobial resistance potential of UTI isolates as well as commensal animal and food isolates. UTI, animal and meat E. coli isolates have been investigated previously for their virulence genes but never to this extent, and only a few isolates were investigated simultaneously for antimicrobial resistance genes (Bonnet et al, 2009;Ewers et al, 2007Ewers et al, , 2009Hamelin et al, 2006Hamelin et al, , 2007Hannah et al, 2009;Jakobsen et al, 2008;Johnson et al, 2005bJohnson et al, , 2009Maynard et al, 2004 The extensive gene profiling, epidemiologically sampled isolates, analysis of B2 isolates only and the many isolate origins investigated here make up the strength of this study and provide radical difference from previous studies. Interestingly, despite this extensive gene profiling, similarities between isolates from UTI, animal and meat E. coli was clearly apparent.…”

Section: Discussionmentioning

confidence: 99%

Microarray-based detection of extended virulence and antimicrobial resistance gene profiles in phylogroup B2 Escherichia coli of human, meat and animal origin

Jakobsen

Garneau

Kurbasic

et al. 2011

Journal of Medical Microbiology

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Extra-intestinal pathogenic Escherichia coli (ExPEC) causing urinary tract infections (UTIs) most often belong to phylogenetic group B2 and stem from the patient's own faecal flora. It has been hypothesized that the external reservoir for these uropathogenic E. coli in the human intestine may be meat and food-production animals. To investigate such a connection, this study analysed an E. coli phylogroup B2 strain collection (n5161) of geographical and temporally matched isolates, published previously, from UTI patients (n552), community-dwelling humans (n536), imported (n55) and Danish (n513) broiler chicken meat, Danish broiler chickens (n517), imported (n53) and Danish (n527) pork, and healthy Danish pigs (n58). The isolates were subjected to microarray analysis for 315 virulence genes and variants and 82 antimicrobial resistance genes and variants. In total, 133 different virulence and antimicrobial resistance genes were detected in at least one UTI isolate. Between 66 and 87 of these genes were also detected in meat and animal isolates. Cluster analyses of virulence and resistance gene profiles, respectively, showed that UTI and community-dwelling human isolates most often grouped with meat and animal isolates, indicating genotypic similarity among such isolates. Furthermore, B2 isolates were detected from UTI patients and meat, with indistinguishable gene profiles. A considerable proportion of the animal and meat isolates belonged to the ExPEC pathotype. In conclusion, these findings suggest that B2 E. coli from meat and animal origin can be the source of most of the virulence and antimicrobial resistance genes detected in uropathogenic E. coli isolates and that there is a general resemblance of animal, meat and UTI E. coli based on extended gene profiling. These findings support the hypothesis of a zoonotic link between E. coli causing UTIs and E. coli from meat and animals.3Dr Brousseau passed away before the completion of the study.4Present address: Steno Diabetes Center A/S, Niels Steensens

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Molecular Analysis of Antimicrobial-Susceptible and -ResistantEscherichia colifrom Retail Meats and Human Stool and Clinical Specimens in a Rural Community Setting

Cited by 35 publications

References 29 publications

Is Escherichia coli urinary tract infection a zoonosis? Proof of direct link with production animals and meat

Is Escherichia coli urinary tract infection a zoonosis? Proof of direct link with production animals and meat

Clonal Composition and Community Clustering of Drug-Susceptible and -Resistant Escherichia coli Isolates from Bloodstream Infections

Microarray-based detection of extended virulence and antimicrobial resistance gene profiles in phylogroup B2 Escherichia coli of human, meat and animal origin

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