Molecular analysis of clinical isolated Pseudomonas aeruginosa by ERIC PCR

Abstract

Pseudomonas aeruginosa is a significant mortality factor due to nosocomial infections in humans. P. aeruginosa has been known with severe infections, high incidence, and multiple drug resistance The present study aims to rapidly diagnose and biotype the isolates of P. aeruginosa isolated from human infections in intensive care units, Al-Sadder Medical City, Al-Hakeem General Hospital, and the burn center in Najaf city by ERIC-PCR. From 156 clinical sample 23 P. aeruginosa were isolated including burn 17/77 (22%), 2/21wound (9.5%), 3/43 urine (6.9%), 1/15 throat swab(6.6) between August 2021 to February 2022. After phenotypic confirmation, isolates were examined by PCR for molecular confirmation Enterobacterial Repetitive Intergenic Consensus (ERIC) PCR were done on 24 isolates. In ERIC-PCR Genetic analysis of P. aeruginosa isolates depending on ≥ 75% similarity revealed the presence of 8 different cluster as figure 2, four of them with single isolate other two clusters with two similar isolates while the two other clusters contained seven identical isolates profile each.