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A total of 156 clinical specimens were collected from patients at Al-Sadder Medical City, Al-Furat al-Awsat Hospital, and the burn center between August 2021 and February 2022. According to medical service utilities, Burn center was the highest with total number of samples with 96 samples and the highest recovery rate 17 isolates followed by Alfurat Al-Awsat Hospital with 32 samples and 4 isolates and finally tame Al-Sadder Medical City with only 28 samples and 2 isolates.  The results of 23 isolates of Pseudomonas aeruginosa revealed that the most effective agent was ciprofloxacin with (60%) resistance which represents regression in the overall potential while Norfloxacin showed high resistance percentage (73%) (The least effective among them) followed by Ofaloxacin (69%) and levofloxacin with (65%).genetically, the result of aac(6´)-Ib-cr gene showed that 21/23 isolates gave positive result with percentage (91.3%).

Whenever the bacterium Acinetobacter Baumannii is mentioned the antimicrobial agents resistance come directly into the mind. This bacteria have been widely distributed worldwide since it have the ability to maintain their living in any surrounding niches including the harsh ones.(Lin, 2014).Being resistant made it a problematic pathogen that makes therapists unable to treat their patients because some of Acinetobacter Baumannii strains are extensively multidrug resistant(XDR) (Moubareck and Halat, 2020)A. Baumannii is characterized as Gram negative coccobacilli has the ability to produce catalase but lacking oxidase and fail to ferment glucose.(Kamali et al., 2020)A. Baumannii can cause wide range of infections comprising various body systems, of which respiratory tract, urinary tract, skin, and blood.(Nwadike, Ojide and Kalu, 2014)

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Pseudomonas aeruginosa is a significant mortality factor due to nosocomial infections in humans. P. aeruginosa has been known with severe infections, high incidence, and multiple drug resistance The present study aims to rapidly diagnose and biotype the isolates of P. aeruginosa isolated from human infections in intensive care units, Al-Sadder Medical City, Al-Hakeem General Hospital, and the burn center in Najaf city by ERIC-PCR. From 156 clinical sample   23 P. aeruginosa were isolated including burn 17/77 (22%), 2/21wound (9.5%), 3/43 urine (6.9%), 1/15 throat swab(6.6) between August 2021 to February 2022. After phenotypic confirmation, isolates were examined by PCR for molecular confirmation Enterobacterial Repetitive Intergenic Consensus (ERIC) PCR were done on 24 isolates. In ERIC-PCR Genetic analysis of P. aeruginosa isolates depending on ≥ 75% similarity revealed the presence of 8 different cluster as figure 2, four of them with single isolate other two clusters with two similar isolates while the two other clusters contained seven identical isolates profile each.