Molecular analysis of cyenopyrafen resistance in the two‐spotted spider mite <i>Tetranychus urticae</i>

Khalighi, Mousaalreza; Dermauw, Wannes; Wybouw, Nicky; Bajda, Sabina; Osakabe, Masahiro; Tirry, Luc; Leeuwen, Thomas Van

doi:10.1002/ps.4071

Cited by 70 publications

(84 citation statements)

References 32 publications

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“…Cyflumetofen was registered in Turkey only two years ago and, together with cyenopyrafen, is the first commercial acaricide that targets Complex II. A potential cross-resistance risk between cyenopyrafen and cyflumetofen has been reported previously (Khalighi et al 2014(Khalighi et al , 2016. Hence, although cyenopyrafen is not registered in Turkey, selection with cyflumetofen could cause cross-resistance in the future.…”

Section: Discussionmentioning

confidence: 95%

Resistance incidence and presence of resistance mutations in populations of Tetranychus urticae from vegetable crops in Turkey

et al. 2019

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Tetranychus urticae Koch is one of the most common and harmful pests in vegetable production areas. Similar to other countries, control of T. urticae is mainly based on acaricides in Turkey. However, T. urticae rapidly develops resistance and failures in chemical control have occurred frequently. The toxicity of various acaricides was investigated in 10 T. urticae populations collected from vegetable crops in Turkey. In addition, populations were screened for the presence of all currently known target-site resistance mutations. It was shown that resistance to bifenthrin was the most widespread, but also half of the populations were resistant to abamectin and hexythiazox. Resistance mutations in the voltage-gated sodium channel (VGSC) and chitin synthase 1 were found in various populations. Moreover, for the first time, F1538I and L1024V VGSC mutations were reported for Turkish populations. Mutations that confer resistance to abamectin, bifenazate and METI-I acaricides such as pyridaben were not detected. These results will contribute to the design of an effective resistance management program in Turkey.

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Section: Discussionmentioning

confidence: 95%

Resistance incidence and presence of resistance mutations in populations of Tetranychus urticae from vegetable crops in Turkey

et al. 2019

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“…In this study, we report for the first time a single mutation in the cd1‐helix, G132A, that confers resistance to bifenazate. The mutation was uncovered after a cross‐resistance screen of JP‐R, a strain of Japanese origin, and was subsequently also detected in a Dutch field strain, FS1. Both lines harbouring the G132A mutation and backcrossed lines displayed similar LC 50 values, and RR and resistance inheritance was perfectly maternal.…”

Section: Discussionmentioning

confidence: 99%

“…During a cross-resistant screen of the Japanese JP-R strain selected for cyenopyrafen resistance, 37 we found strong bifenazate resistance, and therefore sequenced the complete cytochrome b gene. Aligning the cytochrome b sequence of JP-R against those of the susceptible strains Wasatch and GSS revealed a novel amino acid substitution (G132A) ( Table 1 and Fig.…”

Section: Cytochrome B Genotypes Of the Mite Strainsmentioning

confidence: 99%

Identification and characterization of new mutations in mitochondrial cytochrome b that confer resistance to bifenazate and acequinocyl in the spider miteTetranychus urticae

Fotoukkiaii

Tan

Xue

et al. 2019

Pest Management Science

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BACKGROUND In spider mites, mutations in the mitochondrial cytochrome b Qo pocket have been reported to confer resistance to the Qo inhibitors bifenazate and acequinocyl. In this study, we surveyed populations of the two‐spotted spider mite Tetranychus urticae for mutations in cytochrome b, linked newly discovered mutations with resistance and assessed potential pleiotropic fitness costs. RESULTS We identified two novel mutations in the Qo site: G132A (equivalent to G143A in fungi resistant to strobilurins) and G126S + A133T (previously reported to cause bifenazate and acequinocyl resistance in Panonychus citri). Two T. urticae strains carrying G132A were highly resistant to bifenazate but not acequinocyl, whereas a strain with G126S + A133T displayed high levels of acequinocyl resistance, but only moderate levels of bifenazate resistance. Bifenazate and acequinocyl resistance were inherited maternally, providing strong evidence for the involvement of these mutations in the resistance phenotype. Near isogenic lines carrying G132A revealed several fitness penalties in T. urticae; a lower net reproductive rate (R0), intrinsic rate of increase (rm) and finite rate of increase (LM); a higher doubling time (DT); and a more male‐biased sex ratio. CONCLUSIONS Several lines of evidence were provided to support the causal role of newly discovered cytochrome b mutations in bifenazate and acequinocyl resistance. Because of the fitness costs associated with the G132A mutation, resistant T. urticae populations might be less competitive in a bifenazate‐free environment, offering opportunities for resistance management. © 2019 Society of Chemical Industry

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“…Due to its short life cycle, abundant progeny and arrhenotokous reproduction, it develops resistance to various acaricides very rapidly. A large number of studies have presented the resistance of T. urticae to different acaricides …”

Section: Discussionmentioning

confidence: 99%

“…A large number of studies have presented the resistance of T. urticae to different acaricides. [20][21][22][23] In this paper, we reported the resistance selection of bifenthrin, bifenazate and cyflumetofen conducted for one and half years under lab conditions. As bifenthrin was mainly effective on the adult mites, while bifenazate and cyflumetofen were effective on each stage of mite, the population treated with bifenthrin could recover comparatively faster than those treated with bifenazate or cyflumetofen, which was indicated by different selection generation numbers (16 for bifenthrin, 12 for bifenazate or cyflumetofen).…”

Section: Discussionmentioning

confidence: 99%

Laboratory selection, resistance risk assessment, multi‐resistance, and management of Tetranychus urticae Koch to bifenthrin, bifenazate and cyflumetofen on cowpea

Liu

Zhou

Yao

et al. 2020

Pest Management Science

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BACKGROUND Tetranychus urticae (T. urticae) Koch is an important pest of vegetable crops worldwide. In this study, bioassays were carried out to analyze the resistance risk, multi‐resistance and management of T. urticae Koch to bifenthrin, bifenazate and cyflumetofen on cowpea. RESULTS The resistance ratios of the adult T. urticae population to bifenthrin (G16), bifenazate (G12) and cyflumetofen (G12) were 31.29, 9.38 and 5.81, respectively. Realized heritability (h 2) analysis showed that, under a selection pressure of 50–90% mortality, the generations needed to increase 10‐fold LC50 values of bifenthrin, bifenazate and cyflumetofen were 3.64–8.05, 5.75–12.71, and 10.93–24.15, respectively. No obvious multi‐resistance among these three acaricides was observed. Synergist bioassay results showed that microsomal multifunctional oxidase (MFO) was involved in bifenthrin resistance of T. urticae, with a synergistic ratio of 22.38. However, MFO and GSTs were not the main factors conferring the resistance to bifenazate. MFO, glutathione S‐transferases(GSTs), together with esterase contributed to the development of the resistance to cyflumetofen. Additionally, the toxicity selection index test showed that bifenazate was safe to the natural enemy Neoseiulus barkeri (N. barkeri) with a toxicity selection index (TSI) >484.85, while bifenthrin was the least safe (TSI = 0.92). CONCLUSIONS These results demonstrated the T. urticae developed higher resistance risk to bifenthrin compared to bifenazate and cyflumetofen and no obvious multi‐resistance among these three acaricides, providing guidance for designing appropriate strategies for the effective application of bifenthrin, bifenazate and cyflumetofen in the field and delaying the development of insecticide resistance. © 2019 Society of Chemical Industry

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Molecular analysis of cyenopyrafen resistance in the two‐spotted spider mite Tetranychus urticae

Cited by 70 publications

References 32 publications

Resistance incidence and presence of resistance mutations in populations of Tetranychus urticae from vegetable crops in Turkey

Resistance incidence and presence of resistance mutations in populations of Tetranychus urticae from vegetable crops in Turkey

Identification and characterization of new mutations in mitochondrial cytochrome b that confer resistance to bifenazate and acequinocyl in the spider miteTetranychus urticae

Laboratory selection, resistance risk assessment, multi‐resistance, and management of Tetranychus urticae Koch to bifenthrin, bifenazate and cyflumetofen on cowpea

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