Molecular Analysis of Genetic Markers for Non‐Hodgkin Lymphomas

Sholl, Lynette M.; Longtine, Janina A.; Kuo, Frank C.

doi:10.1002/cphg.37

Cited by 2 publications

(2 citation statements)

References 63 publications

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“…All cases were positive for EBV‐encoded small RNA (EBER) by in‐situ hybridization and exhibited either a typical NK cell immunophenotype (CD2 + , surface CD3 − , CD56 + ) or were CD56 − but positive for cytotoxic granule proteins. Haematoxylin and eosin (H&E) and immunohistochemical‐stained slides were examined in all cases, and subsets of cases were analysed by additional immunohistochemistry, flow cytometry and/or molecular genetic analysis using methods described previously and as outlined in further detail in the online Supporting information …”

Section: Methodsmentioning

confidence: 99%

Small‐cell predominant extranodal NK/T cell lymphoma, nasal type: clinicopathological analysis of a series of cases diagnosed in a Western population

et al. 2016

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Aims Extranodal NK/T cell lymphoma, nasal type (ENKTCL) is usually composed of medium‐ to large‐sized lymphoid cells showing prominent angiotrophism and tumour cell necrosis. We report 13 cases composed predominantly of small lymphocytes diagnosed in the United States and Western Europe. Methods and results Patients included seven females and six males aged 17–75 years. Ten presented with sinonasal and three with buccal disease. Nine had stage IE/IIE and four had stage IV disease. In five of seven patients with multiple biopsies at different time‐intervals, the lymphoma was misinterpreted as representing chronic inflammation on an earlier biopsy. In all cases morphology showed a dense infiltrate of small lymphoid cells with minimal cytological atypia. Necrosis, angioinvasion and angiodestruction were each seen in 17%, 22% and 17% of biopsies. Median Ki67 was 5%. Four patients died of lymphoma 4–16 months after diagnosis, including three of four patients with stage IV disease; seven (54%) are alive with no evidence of disease at a median of 39 months; one patient with stage IV disease is alive at 10 months and one recurred at 17 months. Conclusions In sinonasal biopsies with predominantly small lymphocytic infiltrates with admixed chronic inflammation, focal hypercellularity, focal surface ulceration or microscopic bone invasion by small lymphoid cells should alert pathologists to the possibility of small‐cell predominant ENKTCL. Awareness of the full histological spectrum of ENKTCL, particularly in non‐endemic areas, is important in avoiding a delay in diagnosis and ensuring timely initiation of therapy.

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Section: Methodsmentioning

confidence: 99%

Small‐cell predominant extranodal NK/T cell lymphoma, nasal type: clinicopathological analysis of a series of cases diagnosed in a Western population

et al. 2016

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“…The incidence of lymphoma is the eighth highest of all tumors, and the mortality rate is the tenth highest in China. 1,2 Summarizing the molecular genetics 3 and molecular mechanism of lymphoma pathogenesis, 4,5 the type of molecular marker on the surface of lymphocytes, [6][7][8] the gene mutation characteristics of lymphoma cells, 9 and the type and degree of nuclear expression biomolecules in lymphoma cells 9 can assist in the clinical diagnosis of lymphoma. The World Health Organization (WHO) has developed a lymphoma classification standard based on the correlation between the biological phenotype and lymphoma disease.…”

Section: Introductionmentioning

confidence: 99%

Two-way detection of image features and immunolabeling of lymphoma cells with one-step microarray analysis

Zhao

Liu

et al. 2018

Biomicrofluidics

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Detecting the number of pathological lymphoma cells and lymphocyte subtypes in blood is helpful for clinical diagnosis and typing of lymphoma. In the current study, cell type is identified by cell morphological features and immunolabeled lymphocyte subtypes. Red blood cells and leukocytes were separated using a microfluidic cell chip based on physical blood cell parameters, and leukocytes were identified using five characteristic parameters: energy variance, entropy variance, moment of inertia variance, color mean, and cell area individually. The number of red blood cells that could come into contact with the leukocyte membrane was ≤2 based on the microfluidic injection flow rate of microfluidic chips. Anti-CD3 and anti-CD19 antibodies were used for immunofluorescence staining of T-lymphocyte and B-lymphocyte surface antigens, respectively. The results suggested that the microfluidic assay could detect lymphocyte surface antigen markers and intact leukocytes. Therefore, we report a one-step microfluidic chip for classifying hematological lymphoma cells based on the physical parameters of cells, which can simultaneously measure the overall morphology of blood cells and immunolabeling of lymphocyte surface antigens in one step, solving the current problem of detecting subtypes of hematological lymphoma cells based on multiple methods and multi-step detection.

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Molecular Analysis of Genetic Markers for Non‐Hodgkin Lymphomas

Cited by 2 publications

References 63 publications

Small‐cell predominant extranodal NK/T cell lymphoma, nasal type: clinicopathological analysis of a series of cases diagnosed in a Western population

Small‐cell predominant extranodal NK/T cell lymphoma, nasal type: clinicopathological analysis of a series of cases diagnosed in a Western population

Two-way detection of image features and immunolabeling of lymphoma cells with one-step microarray analysis

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