Molecular Analysis of Growth Inhibition Caused by Overexpression of the Biotin Operon in<i>Escherichia coli</i>

Ifuku, Ohji; Koga, Nobuyoshi; Haze, Shinichiro; Kishimoto, Jiro; Aoki, Takayuki; Wachi, Youji

doi:10.1271/bbb.59.184

Cited by 17 publications

(6 citation statements)

References 12 publications

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“…Furthermore, when expression of the Arabidopsis biotin synthase from pBT-1 was induced with isopropylthio-/3-galactoside, growth of the culture was slowed even further regardless of whether the medium for this experiment was Luria broth or M9 (data not shown). These data are consistent with the observation that the overexpression of the E. coli bioB gene is toxic to cells (Ifuku et al, 1995).…”

Section: B Sphaericus --V-dy---egqeans-yr -Led--feie Ltqkq-eafcsupporting

confidence: 92%

Characterization of the cDNA and Gene Coding for the Biotin Synthase of Arabidopsis thaliana

Weaver

Wurtele

et al. 1996

Plant Physiol.

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Biotin, an essential cofactor, is synthesized de novo only by plants and some microbes. An Arabidopsis thaliana expressed sequence tag that shows sequence similarity to the carboxyl end of biotin synthase from Escherichia coli was used to isolate a near-fulllength cDNA. This cDNA was shown to code for the Arabidopsis biotin synthase by its ability to complement a bioB mutant of E. coli.Site-specific mutagenesis indicates that residue threonine-173, which is highly conserved in biotin synthases, is important for catalytic competence of the enzyme. The primary sequence of the Arabidopsis biotin synthase is most similar to biotin synthases from E. coli, Serratia marcescens, and Saccharomyces cerevisiae (about 50% sequence identity) and more distantly related to the Bacillus sphaericus enzyme (33% sequence identity). The primary sequence of the amino terminus of the Arabidopsis biotin synthase may represent an organelle-targeting transit peptide. The single Arabidopsis gene coding for biotin synthase, 9/02, was isolated and sequenced. The biotin synthase coding sequence i s interrupted by five introns. The gene sequence upstream of the translation start site has severa1 unusual features, including imperfect palindromes and polypyrimidine sequences, which may function in the transcriptional regulation of the 9\02 gene.

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Section: B Sphaericus --V-dy---egqeans-yr -Led--feie Ltqkq-eafcsupporting

confidence: 92%

Characterization of the cDNA and Gene Coding for the Biotin Synthase of Arabidopsis thaliana

Weaver

Wurtele

et al. 1996

Plant Physiol.

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“…Plasmid p964 was also the only plasmid containing the entire symbiosis island bio operon that complemented the bioH mutant, albeit to bradytrophic growth, whereas p1013 restored the mutant to wild-type growth. Problems with complementation and\or viability associated with the expression of bioB have been observed in other organisms, including E. coli and Serratia marcescens (Ifuku et al, 1995 ;Levy-Schil et al, 1993 ;Sakurai et al, 1993). Moreover, S. meliloti strains expressing the E. coli bio operon show reduced viability (Streit & Phillips, 1996).…”

Section: Discussionmentioning

confidence: 96%

The bio operon on the acquired symbiosis island of Mesorhizobium sp. strain R7A includes a novel gene involved in pimeloyl-CoA synthesis The GenBank accession number for the sequence reported in this paper is AF311738.

Sullivan

Brown

Yocum³

et al. 2001

Microbiology

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The symbiosis island of Mesorhizobium sp. strain R7A is a 500 kb chromosomal genetic element that upon transfer converts nonsymbiotic mesorhizobia to symbionts able to nodulate and fix nitrogen with Lotus corniculatus. Four genomic species of nonsymbiotic mesorhizobia have been isolated. All were auxotrophic for thiamin and biotin and three were auxotrophic for nicotinate, whereas derivatives of the strains containing the symbiosis island were prototrophic for all three vitamins. In this work, a 132 kb region of the island that converts the nonsymbionts to nicotinate and biotin prototrophy was characterized. The region contained orthologues of the Escherichia coli bioBFD and A genes arranged in an operon with a novel gene, bioZ, a nadABC operon, the nitrogen-fixation regulatory gene nifA, and a homologue of the pantothenate biosynthesis gene panD. The bioZ gene product was similar to β-ketoacyl-acyl carrier protein synthase III (FabH). bioZ ::Tn5 mutants grew poorly in the absence of biotin and the bioZ gene complemented an E. coli bioH mutant, suggesting that its product is involved in the synthesis of pimeloylCoA. The bio operon was not required for symbiosis, as only mutants in the nifA gene were impaired in symbiosis, and a bioA ::Tn5 mutant was not impaired in rhizosphere colonization. The rationale for the vitamin biosynthetic loci being located on an acquired genetic element that is absent from nonsymbiotic mesorhizobia remains to be determined.

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“…Addition of isopropyl /3-D-thiogalactoside, which induces expression of the B1O2 insert in pKK388, also had an inhibitory effect on the growth of these cells (data not shown). The same inhibitory effect is seen in wild-type E. coli cells that harbor on a high-copy plasmid either the wild-type BioB gene or any of several bioB mutant forms with single amino acid substitutions (Ifuku et al, 1995). At this time it is not clear what the precise relationship is between BioB copy number and growth inhibition in E. coli.…”

Section: A T T T C T C a T C T G Pmentioning

confidence: 99%

Biotin Synthase from Arabidopsis thaliana (cDNA Isolation and Characterization of Gene Expression)

1996

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The full-length 8\02 cDNA from Arabidopsis fbaliana was isolated using an expressed sequence tag that was homologous to the Escherichia coli biotin synthase gene (BioB). Comparisons of the deduced amino acid sequence from B 1 0 2 with bacterial and yeast biotin synthase homologs revealed a high degree of sequence similarity. The amino terminus of the predicted B102 protein contains a stretch of hydrophobic residues similar in composition to transit peptide sequences. B 1 0 2 is a single-copy nuclear gene in Arabidopsis that is expressed at high levels in the tissues of immature plants. Expression of B 1 0 2 was higher in the light relative to dark and was induced 5-fold during biotin-limited conditions. These results demonstrate that expression of at least one gene in this pathway is regulated in response to developmental, environmental, and biochemical stimuli.

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Molecular Analysis of Growth Inhibition Caused by Overexpression of the Biotin Operon inEscherichia coli

Cited by 17 publications

References 12 publications

Characterization of the cDNA and Gene Coding for the Biotin Synthase of Arabidopsis thaliana

Characterization of the cDNA and Gene Coding for the Biotin Synthase of Arabidopsis thaliana

The bio operon on the acquired symbiosis island of Mesorhizobium sp. strain R7A includes a novel gene involved in pimeloyl-CoA synthesis The GenBank accession number for the sequence reported in this paper is AF311738.

Biotin Synthase from Arabidopsis thaliana (cDNA Isolation and Characterization of Gene Expression)

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