Molecular analysis of Helicobacter pylori virulent-associated genes in hepatobiliary patients

Abstract

The cagA and cagE genes may be associated in the pathogenesis of hepatobiliary diseases, especially of CCA. Besides the bacterial variation, other host factors may be involved in the pathogenesis of hepatobiliary cancer.

Genetic characterization of Helicobacter pylori vacA and cagA genes in Thai gastro-duodenal and hepatobiliary patients

“…Together, our previous results indicated that H. pylori was strongly associated with CCA through the acceleration of biliary cell inflammation and the disturbance of biliary cell kinetics. We subsequently reported on the genotypes of vacA and cagA genes in hepatobiliary patients [12]. However, it remains unclear whether the gastro-duodenal and hepatobiliary patients suffering from H. pylori infection had the same strain.…”

“…However, it remains unclear whether the gastro-duodenal and hepatobiliary patients suffering from H. pylori infection had the same strain. In the present study, molecular characterization was carried out to determine the genetic characteristics of H. pylori detected in gastro-duodenal diseases and compared with our previous data of hepatobiliary diseases [12]. CCA: Thai cholangiocarcinoma patients; GS: Thai cholelithiasis patients; GT: Thai gastritis patients; GU: Thai gastric ulcer patients; DU: Thai duodenal ulcer patients; GC: Thai gastric cancer patients.…”

“…We have previously reported on the prevalence of H. pylori vacA and cagA genotypes in hepatobiliary patients [12]. However, data on the genetic association between H. pylori colonized in hepatobiliary and gastro-duodenal tracts remain lacking because those genes have not been completely compared between the two disease groups.…”

“…Based on previously published data, bile samples were taken from patients with different hepatobiliary disorders (CCA and cholelithiasis) at the surgical unit of the Srinagarind Hospital, Faculty of Medicine, Khon Kaen University [12]. A total of 80 bile samples were found to be positive for the H. pylori ureA gene via polymerase chain reaction (PCR).…”

“…However, data on the genetic association between H. pylori colonized in hepatobiliary and gastro-duodenal tracts remain lacking because those genes have not been completely compared between the two disease groups. The aims of this study were to characterize the genotypes of the vacA and cagA genes of H. pylori detected in Thai gastro-duodenal patients and to compare these genotypes with Thai hepatobiliary patients based on our previously published data [12]. The genotype patterns of vacA and cagA in these two groups were analyzed.…”