Molecular analysis of intraspecific variation between building and ‘wild’ isolates of Serpula lacrymans and their relatedness to S. himantioides

White, Nia A.; Dehal, P. K.; Duncan, James M.; Williams, N. A.; Gartland, Jill S.; Palfreyman, John W.; Cooke, D. E. L.

doi:10.1017/s0953756201003781

Cited by 29 publications

(35 citation statements)

References 27 publications

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“…himantioides, which has more genetic variation than S. lacrymans, showed polymorphisms among the isolates used in this study, which is in agreement with earlier published sequences of S. himantioides (White et al 2001). Several articles report that S. lacrymans and S. himantioides are distinct taxa.…”

Section: Resultssupporting

confidence: 92%

See 1 more Smart Citation

Identification of Serpula lacrymans and other decay fungi in construction timber by sequencing of ribosomal DNA – A practical approach

Högberg¹,

Land²

2004

Holzforschung

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We have approached species identification of wood decay fungi in construction wood by means of sequencing ribosomal DNA. Sequencing of the Internal Transcribed Spacer (ITS), which includes the 5.8S rDNA, is straightforward and provides a basis for species identification. Identification was either by BLAST search on sequences in GenBank or phylogenetic analysis. A number of important wood decay fungi such as Serpula lacrymans, S. himantioides, Antrodia serialis, A. sinuosa, Gloeophyllum sepiarium, Fomitopsis pinicola, Resinicium bicolor and Junghuhnia collabens have been successfully identified from fruitbodies and directly from wood samples. Sequence variants were found within the species sampled including S. himantioides, the close relative of S. lacrymans. But, among 27 samples from fruitbodies and mycelium of S. lacrymans, unexpectedly, no sequence variation was detected.

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Section: Resultssupporting

confidence: 92%

“…However, other isolates of S. lacrymans showed a rather homogenous RAPD pattern (Schmidt and Moreth 1998). Further studies on isolates of different origin were done by White et al (2001). They analysed wild strains (from Himalaya) and strains from buildings, and concluded that they were genetically similar using RAPDs.…”

Section: Resultsmentioning

confidence: 99%

Identification of Serpula lacrymans and other decay fungi in construction timber by sequencing of ribosomal DNA – A practical approach

Högberg¹,

Land²

2004

Holzforschung

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“…One might speculate that allopatric speciation in South and North America may have happened There is no evidence for a specialization towards growing on wooden constructions, as has apparently happened in S. lacrymans var. lacrymans (Bagchee 1954; Kauserud et al 2007b;White et al 2001). In the phylogenetic tree (Fig 1), isolates derived from nature and buildings appear both in PS1 and PS5 without any apparent structuring, indicating that a constant influx of spores happens from nature to buildings and possibly vice versa.…”

Section: Resultsmentioning

confidence: 99%

Multiple cryptic species with divergent substrate affinities in the Serpula himantioides species complex

Carlsen¹,

Engh²,

Decock³

et al. 2011

Fungal Biology

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“…Long discussions were also done whether also S. lacrymans occurs outside of buildings. Recently it was shown by ITS rDNA sequencing that the fungus occurs in nature in the Himalaya (White et al 2001;Palfreyman et al 2003). A comprehensive phylogenetic analysis based on ITS sequences by Kauserud et al (2004a) showed that isolates of S. lacrymans collected in nature in the Czech Republic, India, Pakistan and Russia group in the branch of indoor isolates ("Domesticus group") but differ from wild Californian isolates ("Shastensis group"), suggesting a North American link between the anthropogenic isolates and the wild relative S. himantioides.…”

Section: Damage and Causal Fungimentioning

confidence: 99%

“…ncbi.nlm.nih.gov/blast). BLAST has revealed the ITSsequence identity of a "wild" S. lacrymans isolate from the Himalayas by comparing it with indoor isolates (White et al 2001), identified misnamed isolates of S. lacrymans (Horisawa et al 2004), identified Antrodia spp. and Serpula spp.…”

Section: Dna-based Techniquesmentioning

confidence: 99%

Indoor wood-decay basidiomycetes: damage, causal fungi, physiology, identification and characterization, prevention and control

Schmidt

2007

Mycol Progress

127

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Indoor wood-decay fungi cause considerable economical damage. Most of the structural damage to the indoors of buildings in Europe and North America is caused by brown-rot fungi that degrade conifer wood; white-rot fungi, which preferentially attack hardwoods, are less common. This review covers the approximately 80 basidiomycetes that commonly occur in buildings. Emphasis was placed on Serpula lacrymans, which is the most common indoor basidiomycete in central Europe. Meruliporia incrassata, the North American pendant to S. lacrymans, has also received considerable attention. In terms of indoor wood decay, moisture and temperature are the most important influences. Wood samples with a low moisture content can be degraded. High temperatures as an alternative control measure do not kill mycelia, with some species surviving in wood samples in the form of heat-resistant arthrospores at temperatures as high 95°C. For refurbishment and scientific purposes, the identity of the causal species should be known. More recently, several molecular techniques have been used to identify fungi; these results are often conflicting with those obtained by other, earlier applied methods. Sequencing of the internal transcribed spacers (ITS) of the rDNA is currently the best molecular tool. Among the other methods available, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) has also been shown to be able to distinguish closely related sister taxa. For further characterization of indoor basidiomycetes, the complete sequences of the 18S, 28S rDNA and the intergenic spacers with the included 5S rDNA have been acquired for some species. If current projects involving whole funal genome sequencing are not taken into account, Antrodia vaillantii is the first basidiomycete for which the complete rDNA sequence has been deposited. The review closes with fundamentals on the prevention and control of indoor wood decay.

show abstract

Molecular analysis of intraspecific variation between building and ‘wild’ isolates of Serpula lacrymans and their relatedness to S. himantioides

Cited by 29 publications

References 27 publications

Identification of Serpula lacrymans and other decay fungi in construction timber by sequencing of ribosomal DNA – A practical approach

Identification of Serpula lacrymans and other decay fungi in construction timber by sequencing of ribosomal DNA – A practical approach

Multiple cryptic species with divergent substrate affinities in the Serpula himantioides species complex

Indoor wood-decay basidiomycetes: damage, causal fungi, physiology, identification and characterization, prevention and control

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